Total data_BA
Methods 20 μL of plasma samples were spiked with deuterated internal standards stock solution. Then proteins were precipitated and supernatants were dried and reconstituted in methanol:water (50:50, V/V). Besides, approximately 50 mg of each tissue were placed in 2 ml tubes containing CK14 ceramic b...
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| Tipo de recurso: | conjunto de datos |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/280249 |
| Acceso en línea: | http://hdl.handle.net/10261/280249 https://doi.org/10.20350/digitalCSIC/14755 |
| Access Level: | acceso abierto |
| Palabra clave: | COX-2 PGE2 BDL Liver Bile Acids Cholestasis |
| Sumario: | Methods 20 μL of plasma samples were spiked with deuterated internal standards stock solution. Then proteins were precipitated and supernatants were dried and reconstituted in methanol:water (50:50, V/V). Besides, approximately 50 mg of each tissue were placed in 2 ml tubes containing CK14 ceramic beads (Precellys). For each 50 mg of tissue, 300 μl of methanol and the deuterated internal standards were added and tissues were homogenized in a Precellys 24 Dual system equipped with a Criolys cooler (Precellys). Samples were analyzed using an Acquity UPLC system (Waters, UK) equipped with an Acquity UPLC BEH C18 column (1.7μm, 2.1 x 100 mm; Waters). The MS analysis was performed using a Waters Xevo TQ-XS mass spectrometer (Waters) with an ESI source working in the negative-ion mode. |
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