TGFb/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands

Signalling by TGFβ superfamily factors plays an important role in tissue growth and cell proliferation. In Drosophila, the activity of the TGFβ/Activin signalling branch has been linked to the regulation of cell growth and proliferation, but the cellular and molecular basis for these functions are n...

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Autores: Martins, Torcato, Eusebio, Nadia, Correia, Andreia, Marinho, Joana, Casares, Fernando, Pereira, Paulo S.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/163623
Acceso en línea:http://hdl.handle.net/10261/163623
Access Level:acceso abierto
Palabra clave:Drosophila
Cell growth
TGFb/Activin
Nucleolus
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repository_id_str
spelling TGFb/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glandsMartins, TorcatoEusebio, NadiaCorreia, AndreiaMarinho, JoanaCasares, FernandoPereira, Paulo S.DrosophilaCell growthTGFb/ActivinNucleolusSignalling by TGFβ superfamily factors plays an important role in tissue growth and cell proliferation. In Drosophila, the activity of the TGFβ/Activin signalling branch has been linked to the regulation of cell growth and proliferation, but the cellular and molecular basis for these functions are not fully understood. In this study, we show that both the RII receptor Punt (Put) and the R-Smad Smad2 are strongly required for cell and tissue growth. Knocking down the expression of Put or Smad2 in salivary glands causes alterations in nucleolar structure and functions. Cells with decreased TGFβ/Activin signalling accumulate intermediate pre-rRNA transcripts containing internal transcribed spacer 1 regions accompanied by the nucleolar retention of ribosomal proteins. Thus, our results show that TGFβ/Activin signalling is required for ribosomal biogenesis, a key aspect of cellular growth control. Importantly, overexpression of Put enhanced cell growth induced by Drosophila Myc, a well-characterized inducer of nucleolar hypertrophy and ribosome biogenesis.This work was supported by project Norte-01-0145-FEDER000029, Advancing Cancer Research: From Basic Knowledge to Application, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and by National Funds through FCT–‘Fundaçâo para a Ciência e a Tecnologia’ under the project FCOMP-01-0124-FEDER015675 (PTDC/SAU-BID/112250/2009). T.M. was supported by an FCT Fellowship ALTF 677-2012, and N.E. was supported by a PhD FCT Fellowship SFRH/BD/95087/2013. P.S.P. is a recipient of a Portuguese ‘Investigator FCT’ contract.Peer reviewedRoyal Society (Great Britain)Fundação para a Ciência e a Tecnologia (Portugal)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/163623reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1098/rsob.160258Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1636232026-05-22T06:33:51Z
dc.title.none.fl_str_mv TGFb/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands
title TGFb/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands
spellingShingle TGFb/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands
Martins, Torcato
Drosophila
Cell growth
TGFb/Activin
Nucleolus
title_short TGFb/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands
title_full TGFb/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands
title_fullStr TGFb/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands
title_full_unstemmed TGFb/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands
title_sort TGFb/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands
dc.creator.none.fl_str_mv Martins, Torcato
Eusebio, Nadia
Correia, Andreia
Marinho, Joana
Casares, Fernando
Pereira, Paulo S.
author Martins, Torcato
author_facet Martins, Torcato
Eusebio, Nadia
Correia, Andreia
Marinho, Joana
Casares, Fernando
Pereira, Paulo S.
author_role author
author2 Eusebio, Nadia
Correia, Andreia
Marinho, Joana
Casares, Fernando
Pereira, Paulo S.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Fundação para a Ciência e a Tecnologia (Portugal)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Drosophila
Cell growth
TGFb/Activin
Nucleolus
topic Drosophila
Cell growth
TGFb/Activin
Nucleolus
description Signalling by TGFβ superfamily factors plays an important role in tissue growth and cell proliferation. In Drosophila, the activity of the TGFβ/Activin signalling branch has been linked to the regulation of cell growth and proliferation, but the cellular and molecular basis for these functions are not fully understood. In this study, we show that both the RII receptor Punt (Put) and the R-Smad Smad2 are strongly required for cell and tissue growth. Knocking down the expression of Put or Smad2 in salivary glands causes alterations in nucleolar structure and functions. Cells with decreased TGFβ/Activin signalling accumulate intermediate pre-rRNA transcripts containing internal transcribed spacer 1 regions accompanied by the nucleolar retention of ribosomal proteins. Thus, our results show that TGFβ/Activin signalling is required for ribosomal biogenesis, a key aspect of cellular growth control. Importantly, overexpression of Put enhanced cell growth induced by Drosophila Myc, a well-characterized inducer of nucleolar hypertrophy and ribosome biogenesis.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/163623
url http://hdl.handle.net/10261/163623
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1098/rsob.160258
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society (Great Britain)
publisher.none.fl_str_mv Royal Society (Great Britain)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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