-509CT/869TC combined genotypes of TGFB1 gene associated with preeclampsia
Preeclampsia is a multisystemic disorder, is part of the spectrum Hypertensive Diseases of Pregnancy; their highest incidences occur in developing countries and is factor of maternal and perinatal morbi- mortality. Transforming Growth Factor β1 (TGF-β1) is a multifunctional cytokine produced during...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | México |
| Institución: | UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO |
| Repositorio: | Revista Internacional de Contaminación Ambiental |
| Idioma: | español |
| OAI Identifier: | oai:ojs.pkp.sfu.ca:article/45974 |
| Acceso en línea: | https://www.revistascca.unam.mx/rica/index.php/rica/article/view/45974 |
| Access Level: | acceso abierto |
| Palabra clave: | preeclampsia single nucleotide polymorphism TGFB1 polimorfismo de un solo nucleótido |
| Sumario: | Preeclampsia is a multisystemic disorder, is part of the spectrum Hypertensive Diseases of Pregnancy; their highest incidences occur in developing countries and is factor of maternal and perinatal morbi- mortality. Transforming Growth Factor β1 (TGF-β1) is a multifunctional cytokine produced during pregnancy mainly by trophoblast cells involved in the regulation of its invasion, proliferation and differentiation. The TGF- β1plasma levels were reported to be increased in preeclampsia. In this study we aimed to investigate the risk of preeclampsia by the combined genotypes -509CT/869TC of the TGFB1 gene in preeclamptic pregnancies of the General Hospital “A” Ministry of Health in Durango, México; we recruited 49 preeclamptic women (cases) and were matched for chronological and gestational age with 100 normoevolutive pregnant women (controls). The polymorphisms were genotyped by real-time PCR. The age of participants was 13 to 24 years, gestational age was 30 to 41.2 weeks. Whereas the T allele of the polymorphism -509C/T was less frequent (0.479) in the group of cases, in control group the frequency of both alleles (C/T) was the same (0.5). For the polymorphism 869T/C, the lower frequency alleles were C = 0.48 in cases and T = 0.45 in controls. Both polymorphisms were in Hardy-Weinberg Balancing. We found nine combined genotypes, the most frequent for both groups was double heterozygous -509CT/869TC with frequency of 0.265 in cases and 0.25 in controls, followed in cases by the combined genotype -509CT/869TT (0.184) and in controls -509TT/869CC (0.15). Using a logistic regression model adjusted for age, -509CT/869TC combined genotype was significantly associated with increased risk of preeclampsia (OR 1.334, 95 % CI 1.024-1.765). The combined genotype -509CC/869CC showed a strong trend of association, but not significant (OR 2.202, 95 % CI 0.983-4.937). Our study is the first that analyze the association of combined genotypes of -509C/T and 869T/C polymorphisms of TGFB1 gene with preeclampsia. |
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