Influence of Preparation Methods of Chitooligosaccharides on Their Physicochemical Properties and Their Anti-Inflammatory Effects in Mice and in RAW264.7 Macrophages

The methods to obtain chitooligosaccharides are tightly related to the physicochemical properties of the end products. Knowledge of these physicochemical characteristics is crucial to describing the biological functions of chitooligosaccharides. Chitooligosaccharides were prepared either in a single...

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Detalhes bibliográficos
Autores: Sánchez, Ángela, Mengíbar, María, Fernández, Margarita, Alemany, Susana, Heras Caballero, Ángeles María, Acosta Contreras, Florentina Niuris
Formato: artículo
Fecha de publicación:2018
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/12708
Acesso em linha:https://hdl.handle.net/20.500.14352/12708
Access Level:acceso abierto
Palavra-chave:615.015.4
chitooligosaccharides
anti-inflammatory action
RAW264.7 macrophage
Biología molecular (Farmacia)
Bioquímica (Farmacia)
Química farmaceútica
2390 Química Farmacéutica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/12708
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network_name_str España
repository_id_str
spelling Influence of Preparation Methods of Chitooligosaccharides on Their Physicochemical Properties and Their Anti-Inflammatory Effects in Mice and in RAW264.7 MacrophagesSánchez, ÁngelaMengíbar, MaríaFernández, MargaritaAlemany, SusanaHeras Caballero, Ángeles MaríaAcosta Contreras, Florentina Niuris615.015.4chitooligosaccharidesanti-inflammatory actionRAW264.7 macrophageBiología molecular (Farmacia)Bioquímica (Farmacia)Química farmaceútica2390 Química FarmacéuticaThe methods to obtain chitooligosaccharides are tightly related to the physicochemical properties of the end products. Knowledge of these physicochemical characteristics is crucial to describing the biological functions of chitooligosaccharides. Chitooligosaccharides were prepared either in a single-step enzymatic hydrolysis using chitosanase, or in a two-step chemical-enzymatic hydrolysis. The hydrolyzed products obtained in the single-step preparation were composed mainly of 42% fully deacetylated oligomers plus 54% monoacetylated oligomers, and they attenuated the inflammation in lipopolysaccharide-induced mice and in RAW264.7 macrophages. However, chitooligosaccharides from the two-step preparation were composed of 50% fully deacetylated oligomers plus 27% monoacetylated oligomers and, conversely, they promoted the inflammatory response in both in vivo and in vitro models. Similar proportions of monoacetylated and deacetylated oligomers is necessary for the mixtures of chitooligosaccharides to achieve anti-inflammatory effects, and it directly depends on the preparation method to which chitosan was submitted.MDPIUniversidad Complutense de Madrid20182018-11-0220182018-11-02journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/12708reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 MAT2010-21016-C02-01 Not availableMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 SAF2014-52009-R Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/127082026-06-02T12:44:21Z
dc.title.none.fl_str_mv Influence of Preparation Methods of Chitooligosaccharides on Their Physicochemical Properties and Their Anti-Inflammatory Effects in Mice and in RAW264.7 Macrophages
title Influence of Preparation Methods of Chitooligosaccharides on Their Physicochemical Properties and Their Anti-Inflammatory Effects in Mice and in RAW264.7 Macrophages
spellingShingle Influence of Preparation Methods of Chitooligosaccharides on Their Physicochemical Properties and Their Anti-Inflammatory Effects in Mice and in RAW264.7 Macrophages
Sánchez, Ángela
615.015.4
chitooligosaccharides
anti-inflammatory action
RAW264.7 macrophage
Biología molecular (Farmacia)
Bioquímica (Farmacia)
Química farmaceútica
2390 Química Farmacéutica
title_short Influence of Preparation Methods of Chitooligosaccharides on Their Physicochemical Properties and Their Anti-Inflammatory Effects in Mice and in RAW264.7 Macrophages
title_full Influence of Preparation Methods of Chitooligosaccharides on Their Physicochemical Properties and Their Anti-Inflammatory Effects in Mice and in RAW264.7 Macrophages
title_fullStr Influence of Preparation Methods of Chitooligosaccharides on Their Physicochemical Properties and Their Anti-Inflammatory Effects in Mice and in RAW264.7 Macrophages
title_full_unstemmed Influence of Preparation Methods of Chitooligosaccharides on Their Physicochemical Properties and Their Anti-Inflammatory Effects in Mice and in RAW264.7 Macrophages
title_sort Influence of Preparation Methods of Chitooligosaccharides on Their Physicochemical Properties and Their Anti-Inflammatory Effects in Mice and in RAW264.7 Macrophages
dc.creator.none.fl_str_mv Sánchez, Ángela
Mengíbar, María
Fernández, Margarita
Alemany, Susana
Heras Caballero, Ángeles María
Acosta Contreras, Florentina Niuris
author Sánchez, Ángela
author_facet Sánchez, Ángela
Mengíbar, María
Fernández, Margarita
Alemany, Susana
Heras Caballero, Ángeles María
Acosta Contreras, Florentina Niuris
author_role author
author2 Mengíbar, María
Fernández, Margarita
Alemany, Susana
Heras Caballero, Ángeles María
Acosta Contreras, Florentina Niuris
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 615.015.4
chitooligosaccharides
anti-inflammatory action
RAW264.7 macrophage
Biología molecular (Farmacia)
Bioquímica (Farmacia)
Química farmaceútica
2390 Química Farmacéutica
topic 615.015.4
chitooligosaccharides
anti-inflammatory action
RAW264.7 macrophage
Biología molecular (Farmacia)
Bioquímica (Farmacia)
Química farmaceútica
2390 Química Farmacéutica
description The methods to obtain chitooligosaccharides are tightly related to the physicochemical properties of the end products. Knowledge of these physicochemical characteristics is crucial to describing the biological functions of chitooligosaccharides. Chitooligosaccharides were prepared either in a single-step enzymatic hydrolysis using chitosanase, or in a two-step chemical-enzymatic hydrolysis. The hydrolyzed products obtained in the single-step preparation were composed mainly of 42% fully deacetylated oligomers plus 54% monoacetylated oligomers, and they attenuated the inflammation in lipopolysaccharide-induced mice and in RAW264.7 macrophages. However, chitooligosaccharides from the two-step preparation were composed of 50% fully deacetylated oligomers plus 27% monoacetylated oligomers and, conversely, they promoted the inflammatory response in both in vivo and in vitro models. Similar proportions of monoacetylated and deacetylated oligomers is necessary for the mixtures of chitooligosaccharides to achieve anti-inflammatory effects, and it directly depends on the preparation method to which chitosan was submitted.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-11-02
2018
2018-11-02
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/12708
url https://hdl.handle.net/20.500.14352/12708
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 MAT2010-21016-C02-01 Not available
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 SAF2014-52009-R Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869415735073177600
score 15.301629

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