Comparing stunning methods and seasonal effects on biochemical variables and product quality of rainbow trout

This study compared live chilling (LC) and dry electrical stunning at higher (HI: 400 mA for 0.5 s followed by 200 mA for 1.5 s) and lower (LI: 200 mA for 2 s) intensities in combination with live chilling in rainbow trout (Oncorhynchus mykiss), evaluating their impact on muscle damage, metabolism,...

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Autores: González Garoz, Roberto, Cabezas Albéniz, Almudena, González De Chávarri Echániz, Elisabeth, Fernández-Muela Garrote, Montserrat, Martínez Villalba, Andrea, Villarroel, Morris, de la Llave-Propín, Álvaro, Fuente Vázquez, Jesús De La, Bermejo Poza, Rubén, Díaz Díaz Chirón, María Teresa
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/124898
Acceso en línea:https://hdl.handle.net/20.500.14352/124898
Access Level:acceso abierto
Palabra clave:639.3
Rainbow trout
Product quality
Live chilling
Dry electric stunning
Seasonality
Piscicultura
3105.02 Piscicultura
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repository_id_str
spelling Comparing stunning methods and seasonal effects on biochemical variables and product quality of rainbow troutGonzález Garoz, RobertoCabezas Albéniz, AlmudenaGonzález De Chávarri Echániz, ElisabethFernández-Muela Garrote, MontserratMartínez Villalba, AndreaVillarroel, Morrisde la Llave-Propín, ÁlvaroFuente Vázquez, Jesús De LaBermejo Poza, RubénDíaz Díaz Chirón, María Teresa639.3Rainbow troutProduct qualityLive chillingDry electric stunningSeasonalityPiscicultura3105.02 PisciculturaThis study compared live chilling (LC) and dry electrical stunning at higher (HI: 400 mA for 0.5 s followed by 200 mA for 1.5 s) and lower (LI: 200 mA for 2 s) intensities in combination with live chilling in rainbow trout (Oncorhynchus mykiss), evaluating their impact on muscle damage, metabolism, and sensory attributes under winter and summer. Trout were stunned, slaughtered and samples were taken for biochemical markers, muscle pH, rigor mortis, texture, color and freshness analysis. Our results showed that stunning method and seasonal conditions significantly influenced product quality, with low-intensity electrical stunning being most effective under warmer conditions. Blood levels of lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) were highest in LC and HI groups, indicating greater muscle damage, compared to LI. Osmolality was also elevated in HI, suggesting increased cell damage. Seasonality influenced muscle metabolism: summer results, compared with winter, showed lower glycogen reserves and reduced glycolytic enzyme activity (lower HK, PK, LDH activity), along with increased gluconeogenesis (higher FBP activity), and also altered oxidative stress, evidenced by up-regulated sod and gst gene expression and down-regulated gpx and cat expression in muscle. Muscle pH at 0 h post-mortem was lowest in LC, while rigor mortis developed faster in this group in summer. Texture analysis showed an interaction between stunning method and season, with slightly higher cohesiveness in LI group in summer. Seasonal effects on texture included lower hardness and springiness but higher adhesiveness in summer. Despite differences, no significant variations in color stability or freshness between stunning methods were observedElsevier B.VUniversidad Complutense de Madrid20252025-01-0120252025-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/124898reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1248982026-06-02T12:44:21Z
dc.title.none.fl_str_mv Comparing stunning methods and seasonal effects on biochemical variables and product quality of rainbow trout
title Comparing stunning methods and seasonal effects on biochemical variables and product quality of rainbow trout
spellingShingle Comparing stunning methods and seasonal effects on biochemical variables and product quality of rainbow trout
González Garoz, Roberto
639.3
Rainbow trout
Product quality
Live chilling
Dry electric stunning
Seasonality
Piscicultura
3105.02 Piscicultura
title_short Comparing stunning methods and seasonal effects on biochemical variables and product quality of rainbow trout
title_full Comparing stunning methods and seasonal effects on biochemical variables and product quality of rainbow trout
title_fullStr Comparing stunning methods and seasonal effects on biochemical variables and product quality of rainbow trout
title_full_unstemmed Comparing stunning methods and seasonal effects on biochemical variables and product quality of rainbow trout
title_sort Comparing stunning methods and seasonal effects on biochemical variables and product quality of rainbow trout
dc.creator.none.fl_str_mv González Garoz, Roberto
Cabezas Albéniz, Almudena
González De Chávarri Echániz, Elisabeth
Fernández-Muela Garrote, Montserrat
Martínez Villalba, Andrea
Villarroel, Morris
de la Llave-Propín, Álvaro
Fuente Vázquez, Jesús De La
Bermejo Poza, Rubén
Díaz Díaz Chirón, María Teresa
author González Garoz, Roberto
author_facet González Garoz, Roberto
Cabezas Albéniz, Almudena
González De Chávarri Echániz, Elisabeth
Fernández-Muela Garrote, Montserrat
Martínez Villalba, Andrea
Villarroel, Morris
de la Llave-Propín, Álvaro
Fuente Vázquez, Jesús De La
Bermejo Poza, Rubén
Díaz Díaz Chirón, María Teresa
author_role author
author2 Cabezas Albéniz, Almudena
González De Chávarri Echániz, Elisabeth
Fernández-Muela Garrote, Montserrat
Martínez Villalba, Andrea
Villarroel, Morris
de la Llave-Propín, Álvaro
Fuente Vázquez, Jesús De La
Bermejo Poza, Rubén
Díaz Díaz Chirón, María Teresa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 639.3
Rainbow trout
Product quality
Live chilling
Dry electric stunning
Seasonality
Piscicultura
3105.02 Piscicultura
topic 639.3
Rainbow trout
Product quality
Live chilling
Dry electric stunning
Seasonality
Piscicultura
3105.02 Piscicultura
description This study compared live chilling (LC) and dry electrical stunning at higher (HI: 400 mA for 0.5 s followed by 200 mA for 1.5 s) and lower (LI: 200 mA for 2 s) intensities in combination with live chilling in rainbow trout (Oncorhynchus mykiss), evaluating their impact on muscle damage, metabolism, and sensory attributes under winter and summer. Trout were stunned, slaughtered and samples were taken for biochemical markers, muscle pH, rigor mortis, texture, color and freshness analysis. Our results showed that stunning method and seasonal conditions significantly influenced product quality, with low-intensity electrical stunning being most effective under warmer conditions. Blood levels of lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) were highest in LC and HI groups, indicating greater muscle damage, compared to LI. Osmolality was also elevated in HI, suggesting increased cell damage. Seasonality influenced muscle metabolism: summer results, compared with winter, showed lower glycogen reserves and reduced glycolytic enzyme activity (lower HK, PK, LDH activity), along with increased gluconeogenesis (higher FBP activity), and also altered oxidative stress, evidenced by up-regulated sod and gst gene expression and down-regulated gpx and cat expression in muscle. Muscle pH at 0 h post-mortem was lowest in LC, while rigor mortis developed faster in this group in summer. Texture analysis showed an interaction between stunning method and season, with slightly higher cohesiveness in LI group in summer. Seasonal effects on texture included lower hardness and springiness but higher adhesiveness in summer. Despite differences, no significant variations in color stability or freshness between stunning methods were observed
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/124898
url https://hdl.handle.net/20.500.14352/124898
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V
publisher.none.fl_str_mv Elsevier B.V
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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