Antioxidant and genoprotective effects of spent coffee extracts in human cells

Spent coffee has been shown as a good source of hydrophilic antioxidant compounds. The ability of two spent coffee extracts rich in caffeoylquinic acids, mainly dicaffeoylquinic acids, and caffeine (Arabica filter and Robusta espresso) to protect against oxidation and DNA damage in human cells (HeLa...

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Autores: Bravo, J. (Jimena)|||/items/8a0c6a82-67a1-47c6-ad4e-684d8568fc4e, Arbillaga, L. (Leire)|||/items/5d121cf6-ae3a-49f1-853d-2bcd4588ff39, Peña, M.P. (María Paz) de|||/items/573639ac-cfd4-4c83-ad42-32def0ce5522, Cid-Canda, C. (Concepción)|||/items/e0b35b0b-38b6-46f6-87fa-ee5cf58bf903
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/35038
Acceso en línea:https://hdl.handle.net/10171/35038
Access Level:acceso abierto
Palabra clave:Coffee
Spent coffee
Antioxidant
Antigenotoxicity
DNA damage
Comet assay
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spelling Antioxidant and genoprotective effects of spent coffee extracts in human cellsBravo, J. (Jimena)|||/items/8a0c6a82-67a1-47c6-ad4e-684d8568fc4eArbillaga, L. (Leire)|||/items/5d121cf6-ae3a-49f1-853d-2bcd4588ff39Peña, M.P. (María Paz) de|||/items/573639ac-cfd4-4c83-ad42-32def0ce5522Cid-Canda, C. (Concepción)|||/items/e0b35b0b-38b6-46f6-87fa-ee5cf58bf903CoffeeSpent coffeeAntioxidantAntigenotoxicityDNA damageComet assayComet assaySpent coffee has been shown as a good source of hydrophilic antioxidant compounds. The ability of two spent coffee extracts rich in caffeoylquinic acids, mainly dicaffeoylquinic acids, and caffeine (Arabica filter and Robusta espresso) to protect against oxidation and DNA damage in human cells (HeLa) was evaluated at short (2 h) and long (24 h) exposure times. Cell viability (MTT) was not affected by spent coffee extracts (>80%) up to 1000 mu g/mL after 2 h. Both spent coffee extracts significantly reduced the increase of ROS level and DNA strand breaks (29-73% protection by comet assay) induced by H2O2. Pretreatment of cells with robusta spent coffee extract also decreased Ro photosensitizer-induced oxidative DNA damage after 24 h exposure. The higher effectiveness of Robusta spent coffee extract, with less caffeoylquinic acids and melanoidins, might be due to other antioxidant compounds, such as caffeine and other Maillard reaction products. This work evidences the potential antioxidant and genoprotective properties of spent coffee in human cells.ElsevierDadun. Depósito Académico Digital Universidad de Navarra20142014-02-0320132013-01-0120132013-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/35038reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/350382026-06-21T12:47:57Z
dc.title.none.fl_str_mv Antioxidant and genoprotective effects of spent coffee extracts in human cells
title Antioxidant and genoprotective effects of spent coffee extracts in human cells
spellingShingle Antioxidant and genoprotective effects of spent coffee extracts in human cells
Bravo, J. (Jimena)|||/items/8a0c6a82-67a1-47c6-ad4e-684d8568fc4e
Coffee
Spent coffee
Antioxidant
Antigenotoxicity
DNA damage
Comet assay
Comet assay
title_short Antioxidant and genoprotective effects of spent coffee extracts in human cells
title_full Antioxidant and genoprotective effects of spent coffee extracts in human cells
title_fullStr Antioxidant and genoprotective effects of spent coffee extracts in human cells
title_full_unstemmed Antioxidant and genoprotective effects of spent coffee extracts in human cells
title_sort Antioxidant and genoprotective effects of spent coffee extracts in human cells
dc.creator.none.fl_str_mv Bravo, J. (Jimena)|||/items/8a0c6a82-67a1-47c6-ad4e-684d8568fc4e
Arbillaga, L. (Leire)|||/items/5d121cf6-ae3a-49f1-853d-2bcd4588ff39
Peña, M.P. (María Paz) de|||/items/573639ac-cfd4-4c83-ad42-32def0ce5522
Cid-Canda, C. (Concepción)|||/items/e0b35b0b-38b6-46f6-87fa-ee5cf58bf903
author Bravo, J. (Jimena)|||/items/8a0c6a82-67a1-47c6-ad4e-684d8568fc4e
author_facet Bravo, J. (Jimena)|||/items/8a0c6a82-67a1-47c6-ad4e-684d8568fc4e
Arbillaga, L. (Leire)|||/items/5d121cf6-ae3a-49f1-853d-2bcd4588ff39
Peña, M.P. (María Paz) de|||/items/573639ac-cfd4-4c83-ad42-32def0ce5522
Cid-Canda, C. (Concepción)|||/items/e0b35b0b-38b6-46f6-87fa-ee5cf58bf903
author_role author
author2 Arbillaga, L. (Leire)|||/items/5d121cf6-ae3a-49f1-853d-2bcd4588ff39
Peña, M.P. (María Paz) de|||/items/573639ac-cfd4-4c83-ad42-32def0ce5522
Cid-Canda, C. (Concepción)|||/items/e0b35b0b-38b6-46f6-87fa-ee5cf58bf903
author2_role author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Coffee
Spent coffee
Antioxidant
Antigenotoxicity
DNA damage
Comet assay
Comet assay
topic Coffee
Spent coffee
Antioxidant
Antigenotoxicity
DNA damage
Comet assay
Comet assay
description Spent coffee has been shown as a good source of hydrophilic antioxidant compounds. The ability of two spent coffee extracts rich in caffeoylquinic acids, mainly dicaffeoylquinic acids, and caffeine (Arabica filter and Robusta espresso) to protect against oxidation and DNA damage in human cells (HeLa) was evaluated at short (2 h) and long (24 h) exposure times. Cell viability (MTT) was not affected by spent coffee extracts (>80%) up to 1000 mu g/mL after 2 h. Both spent coffee extracts significantly reduced the increase of ROS level and DNA strand breaks (29-73% protection by comet assay) induced by H2O2. Pretreatment of cells with robusta spent coffee extract also decreased Ro photosensitizer-induced oxidative DNA damage after 24 h exposure. The higher effectiveness of Robusta spent coffee extract, with less caffeoylquinic acids and melanoidins, might be due to other antioxidant compounds, such as caffeine and other Maillard reaction products. This work evidences the potential antioxidant and genoprotective properties of spent coffee in human cells.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01
2013
2013-01-01
2014
2014-02-03
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/35038
url https://hdl.handle.net/10171/35038
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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