Unraveling the role of transient starch in the response of Arabidopsis to elevated CO2 under long-day conditions

Previous studies on Arabidopsis under long-term exposure to elevated CO2 have been conducted using starch synthesis and breakdown mutants cultured under short day conditions. These studies showed that starch synthesis can ameliorate the photosynthetic reduction caused by soluble sugar-mediated feedb...

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Autores: Jáuregui Mosquera, Iván, Pozueta Romero, Javier, Aparicio Tejo, Pedro María, Baroja Fernández, Edurne, Aranjuelo Michelena, Iker
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/38635
Acceso en línea:https://hdl.handle.net/2454/38635
Access Level:acceso abierto
Palabra clave:Starch
Elevated CO2
Photosynthesis
Growth
Photosynthetic acclimation
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spelling Unraveling the role of transient starch in the response of Arabidopsis to elevated CO2 under long-day conditionsJáuregui Mosquera, IvánPozueta Romero, JavierAparicio Tejo, Pedro MaríaBaroja Fernández, EdurneAranjuelo Michelena, IkerStarchElevated CO2PhotosynthesisGrowthPhotosynthetic acclimationPrevious studies on Arabidopsis under long-term exposure to elevated CO2 have been conducted using starch synthesis and breakdown mutants cultured under short day conditions. These studies showed that starch synthesis can ameliorate the photosynthetic reduction caused by soluble sugar-mediated feedback regulation. In this work we characterized the effect of long-term exposure to elevated CO2 (800 ppm) on growth, photosynthesis and content of primary photosynthates in long-day grown wild type plants as well as the near starch-less (aps1) and the starch-excess (gwd) mutants. Notably, elevated CO2 promoted growth of both wild type and aps1 plants but had no effect on gwd plants. Growth promotion by elevated CO2 was accompanied by an increased net photosynthesis in WT and aps1 plants. However, the plants with the highest starch content (wild type at elevated CO2, gwd at ambient CO2, and gwd at elevated CO2) were the ones that suffered decreased in in vivo maximum carboxylation rate of Rubisco, and therefore, photosynthetic down-regulation. Further, the photosynthetic rates of wild type at elevated CO2 and gwd at elevated CO2 were acclimated to elevated CO2. Notably, elevated CO2 promoted the accumulation of stress-responsive and senescence-associated amino acid markers in gwd plants. The results presented in this work provide evidence that under long-day conditions, temporary storage of overflow photosynthate as starch negatively affect Rubisco performance. These data are consistent with earlier hypothesis that photosynthetic acclimation can be caused by accelerated senescence and hindrance of CO2 diffusion to the stroma due to accumulation of large starch granules.This work was partially supported by the Spanish National Research and Development Programme (AGL2016-79868-R), by Comisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain) (grant number BIO2016-78747-P) and the Basque Government (IT-932-16). The authors would like to acknowledge the technical support provided by Dr. Philippe D'Hooghe from the UMR INRA/UCN 950 Ecophysiologie Végétale et Agronomy (Université de Caen Normandie).ElsevierZientziakCienciasIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2454/38635reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/ES/1PE/AGL2016-79868-Rinfo:eu-repo/grantAgreement/ES/1PE/BIO2016-78747-P© 2018 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/386352026-06-17T12:41:47Z
dc.title.none.fl_str_mv Unraveling the role of transient starch in the response of Arabidopsis to elevated CO2 under long-day conditions
title Unraveling the role of transient starch in the response of Arabidopsis to elevated CO2 under long-day conditions
spellingShingle Unraveling the role of transient starch in the response of Arabidopsis to elevated CO2 under long-day conditions
Jáuregui Mosquera, Iván
Starch
Elevated CO2
Photosynthesis
Growth
Photosynthetic acclimation
title_short Unraveling the role of transient starch in the response of Arabidopsis to elevated CO2 under long-day conditions
title_full Unraveling the role of transient starch in the response of Arabidopsis to elevated CO2 under long-day conditions
title_fullStr Unraveling the role of transient starch in the response of Arabidopsis to elevated CO2 under long-day conditions
title_full_unstemmed Unraveling the role of transient starch in the response of Arabidopsis to elevated CO2 under long-day conditions
title_sort Unraveling the role of transient starch in the response of Arabidopsis to elevated CO2 under long-day conditions
dc.creator.none.fl_str_mv Jáuregui Mosquera, Iván
Pozueta Romero, Javier
Aparicio Tejo, Pedro María
Baroja Fernández, Edurne
Aranjuelo Michelena, Iker
author Jáuregui Mosquera, Iván
author_facet Jáuregui Mosquera, Iván
Pozueta Romero, Javier
Aparicio Tejo, Pedro María
Baroja Fernández, Edurne
Aranjuelo Michelena, Iker
author_role author
author2 Pozueta Romero, Javier
Aparicio Tejo, Pedro María
Baroja Fernández, Edurne
Aranjuelo Michelena, Iker
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Zientziak
Ciencias
IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
dc.subject.none.fl_str_mv Starch
Elevated CO2
Photosynthesis
Growth
Photosynthetic acclimation
topic Starch
Elevated CO2
Photosynthesis
Growth
Photosynthetic acclimation
description Previous studies on Arabidopsis under long-term exposure to elevated CO2 have been conducted using starch synthesis and breakdown mutants cultured under short day conditions. These studies showed that starch synthesis can ameliorate the photosynthetic reduction caused by soluble sugar-mediated feedback regulation. In this work we characterized the effect of long-term exposure to elevated CO2 (800 ppm) on growth, photosynthesis and content of primary photosynthates in long-day grown wild type plants as well as the near starch-less (aps1) and the starch-excess (gwd) mutants. Notably, elevated CO2 promoted growth of both wild type and aps1 plants but had no effect on gwd plants. Growth promotion by elevated CO2 was accompanied by an increased net photosynthesis in WT and aps1 plants. However, the plants with the highest starch content (wild type at elevated CO2, gwd at ambient CO2, and gwd at elevated CO2) were the ones that suffered decreased in in vivo maximum carboxylation rate of Rubisco, and therefore, photosynthetic down-regulation. Further, the photosynthetic rates of wild type at elevated CO2 and gwd at elevated CO2 were acclimated to elevated CO2. Notably, elevated CO2 promoted the accumulation of stress-responsive and senescence-associated amino acid markers in gwd plants. The results presented in this work provide evidence that under long-day conditions, temporary storage of overflow photosynthate as starch negatively affect Rubisco performance. These data are consistent with earlier hypothesis that photosynthetic acclimation can be caused by accelerated senescence and hindrance of CO2 diffusion to the stroma due to accumulation of large starch granules.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/38635
url https://hdl.handle.net/2454/38635
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/ES/1PE/AGL2016-79868-R
info:eu-repo/grantAgreement/ES/1PE/BIO2016-78747-P
dc.rights.none.fl_str_mv © 2018 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0
https://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2018 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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