Contaminant DNA in bacterial sequencing experiments is a major source of false genetic variability

[Background] Contaminant DNA is a well-known confounding factor in molecular biology and in genomic repositories. Strikingly, analysis workflows for whole-genome sequencing (WGS) data commonly do not account for errors potentially introduced by contamination, which could lead to the wrong assessment...

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Detalles Bibliográficos
Autores: Goig, Galo A., Blanco, Silvia, Garcia-Basteiro, Alberto L., Comas, Iñaki
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/203109
Acceso en línea:http://hdl.handle.net/10261/203109
Access Level:acceso abierto
Descripción
Sumario:[Background] Contaminant DNA is a well-known confounding factor in molecular biology and in genomic repositories. Strikingly, analysis workflows for whole-genome sequencing (WGS) data commonly do not account for errors potentially introduced by contamination, which could lead to the wrong assessment of allele frequency both in basic and clinical research.