Temporal expression patterns of the melatoninergic system in the human thymus of children

Cruz Chamorro, Iván; Álvarez Sánchez, Nuria; Escalante Andicoechea, Cristina; Carrillo Vico, Antonio; Rubio, Amalia; Guerrero, Juan Miguel; Molinero, Patrocinio; Lardone, Patricia Judith

Temporal expression patterns of the melatoninergic system in the human thymus of children

Objectives: To obtain greater knowledge of the extra-pineal sources of melatonin during development, the amount of indolamine and the expression levels of the last two enzymes involved in its biosynthesis, Arylalkylamine N-acetyltransferase (AANAT) and acetylserotonin Omethyltransferase (ASMT), were...

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Autores:	Cruz Chamorro, Iván, Álvarez Sánchez, Nuria, Escalante Andicoechea, Cristina, Carrillo Vico, Antonio, Rubio, Amalia, Guerrero, Juan Miguel, Molinero, Patrocinio, Lardone, Patricia Judith
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2019
País:	España
Institución:	Universidad de Sevilla (US)
Repositorio:	idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:	oai:idus.us.es:11441/102275
Acceso en línea:	https://hdl.handle.net/11441/102275 https://doi.org/10.1016/j.molmet.2019.07.007
Access Level:	acceso abierto
Palabra clave:	Melatonin Thymus AANAT ASMT Melatonin receptor Nuclear receptor ROR-alpha

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spelling	Temporal expression patterns of the melatoninergic system in the human thymus of childrenCruz Chamorro, IvánÁlvarez Sánchez, NuriaEscalante Andicoechea, CristinaCarrillo Vico, AntonioRubio, AmaliaGuerrero, Juan MiguelMolinero, PatrocinioLardone, Patricia JudithMelatoninThymusAANATASMTMelatonin receptorNuclear receptor ROR-alphaObjectives: To obtain greater knowledge of the extra-pineal sources of melatonin during development, the amount of indolamine and the expression levels of the last two enzymes involved in its biosynthesis, Arylalkylamine N-acetyltransferase (AANAT) and acetylserotonin Omethyltransferase (ASMT), were analyzed in the human thymus from children from three different age groups (from days to years). The melatonin membrane and nuclear receptor expression levels also were studied. Methods: Quantitative reverse transcriptase PCR and western blot were performed to investigate the receptor and enzyme expression levels. The results were examined and correlated with the ages of the thymuses. Results: We found high levels of indolamine in the thymuses of newborns (younger than 1 month), which decreased during development; thymuses from the months (from 2 to 11 months) and years (from 1 to 12 years) groups showed lower levels. A similar decline was also observed in the mRNA of the AANAT enzyme and the expression levels of melatonin receptors. However, ASMT expression was exactly the opposite, with low levels in the newborn group and higher levels in the years group. Our results show that the thymic synthesis of melatonin occurs very early in childhood. Additionally, this is the first report that is focused on melatonin receptors expression in the human thymus. Conclusion: Considering the limited melatonin synthesis performed by the newborn pineal gland, we suggest that the high levels of melatonin found in human thymus in this experimental group arise from synthesis in the tissue itself, which could be contributing to the immune efficiency at the thymic level.Regional Government Ministry of Health PI-0209-2010PAIDI Program from the Andalusian Government CTS160Andalusian Public Foundation Progress and Health PI-0485-2014FPU programSpanish Government FPU13/01210Instituto de Salud Carlos III, Ministerio de Economía y Competitividad RD06/ 0013/0001 and RD12/0043/0012Premio Anual Publicación Científica Destacada de la US. Facultad de MedicinaPremio Mensual Publicación Científica Destacada de la US. Facultad de MedicinaElsevier BVBioquímica Médica y Biología Molecular e Inmunología2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/102275https://doi.org/10.1016/j.molmet.2019.07.007reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMolecular Metabolism, 28.PI-0209-2010CTS160PI-0485-2014FPU13/01210RD06/ 0013/0001RD12/0043/0012https://doi.org/10.1016/j.molmet.2019.07.007info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1022752026-06-17T12:51:07Z
dc.title.none.fl_str_mv	Temporal expression patterns of the melatoninergic system in the human thymus of children
title	Temporal expression patterns of the melatoninergic system in the human thymus of children
spellingShingle	Temporal expression patterns of the melatoninergic system in the human thymus of children Cruz Chamorro, Iván Melatonin Thymus AANAT ASMT Melatonin receptor Nuclear receptor ROR-alpha
title_short	Temporal expression patterns of the melatoninergic system in the human thymus of children
title_full	Temporal expression patterns of the melatoninergic system in the human thymus of children
title_fullStr	Temporal expression patterns of the melatoninergic system in the human thymus of children
title_full_unstemmed	Temporal expression patterns of the melatoninergic system in the human thymus of children
title_sort	Temporal expression patterns of the melatoninergic system in the human thymus of children
dc.creator.none.fl_str_mv	Cruz Chamorro, Iván Álvarez Sánchez, Nuria Escalante Andicoechea, Cristina Carrillo Vico, Antonio Rubio, Amalia Guerrero, Juan Miguel Molinero, Patrocinio Lardone, Patricia Judith
author	Cruz Chamorro, Iván
author_facet	Cruz Chamorro, Iván Álvarez Sánchez, Nuria Escalante Andicoechea, Cristina Carrillo Vico, Antonio Rubio, Amalia Guerrero, Juan Miguel Molinero, Patrocinio Lardone, Patricia Judith
author_role	author
author2	Álvarez Sánchez, Nuria Escalante Andicoechea, Cristina Carrillo Vico, Antonio Rubio, Amalia Guerrero, Juan Miguel Molinero, Patrocinio Lardone, Patricia Judith
author2_role	author author author author author author author
dc.contributor.none.fl_str_mv	Bioquímica Médica y Biología Molecular e Inmunología
dc.subject.none.fl_str_mv	Melatonin Thymus AANAT ASMT Melatonin receptor Nuclear receptor ROR-alpha
topic	Melatonin Thymus AANAT ASMT Melatonin receptor Nuclear receptor ROR-alpha
description	Objectives: To obtain greater knowledge of the extra-pineal sources of melatonin during development, the amount of indolamine and the expression levels of the last two enzymes involved in its biosynthesis, Arylalkylamine N-acetyltransferase (AANAT) and acetylserotonin Omethyltransferase (ASMT), were analyzed in the human thymus from children from three different age groups (from days to years). The melatonin membrane and nuclear receptor expression levels also were studied. Methods: Quantitative reverse transcriptase PCR and western blot were performed to investigate the receptor and enzyme expression levels. The results were examined and correlated with the ages of the thymuses. Results: We found high levels of indolamine in the thymuses of newborns (younger than 1 month), which decreased during development; thymuses from the months (from 2 to 11 months) and years (from 1 to 12 years) groups showed lower levels. A similar decline was also observed in the mRNA of the AANAT enzyme and the expression levels of melatonin receptors. However, ASMT expression was exactly the opposite, with low levels in the newborn group and higher levels in the years group. Our results show that the thymic synthesis of melatonin occurs very early in childhood. Additionally, this is the first report that is focused on melatonin receptors expression in the human thymus. Conclusion: Considering the limited melatonin synthesis performed by the newborn pineal gland, we suggest that the high levels of melatonin found in human thymus in this experimental group arise from synthesis in the tissue itself, which could be contributing to the immune efficiency at the thymic level.
publishDate	2019
dc.date.none.fl_str_mv	2019
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://hdl.handle.net/11441/102275 https://doi.org/10.1016/j.molmet.2019.07.007
url	https://hdl.handle.net/11441/102275 https://doi.org/10.1016/j.molmet.2019.07.007
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Molecular Metabolism, 28. PI-0209-2010 CTS160 PI-0485-2014 FPU13/01210 RD06/ 0013/0001 RD12/0043/0012 https://doi.org/10.1016/j.molmet.2019.07.007
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Elsevier BV
publisher.none.fl_str_mv	Elsevier BV
dc.source.none.fl_str_mv	reponame:idUS. Depósito de Investigación de la Universidad de Sevilla instname:Universidad de Sevilla (US)
instname_str	Universidad de Sevilla (US)
reponame_str	idUS. Depósito de Investigación de la Universidad de Sevilla
collection	idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,300724

Temporal expression patterns of the melatoninergic system in the human thymus of children

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