Production of polyhydroxyalkanoates (PHAs) for use as biodegradable capsules in the pharmaceutical industry

The objective of this project has been the study of a biopolymer produced by microorganisms from waste, with biodegradability and biocompatibility properties, for use as a nanocapsule/nanoparticle in the pharmaceutical industry. The nanoparticles, made from Poly(3-hydroxybutyrate-co-3- hydroxyvalera...

Descripción completa

Detalles Bibliográficos
Autor: Rodríguez Pájaro, Jorge
Tipo de recurso: tesis de maestría
Fecha de publicación:2023
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/396448
Acceso en línea:https://hdl.handle.net/2117/396448
Access Level:acceso abierto
Palabra clave:Biodegradable plastics -- Testing
Biopolymers
Pharmaceutical industry
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
nanoparticles
biodregradable
pharmaceutical and medical industry
Plàstics biodegradables -- Proves
Biopolímers
Indústria farmacèutica
Àrees temàtiques de la UPC::Enginyeria química
id ES_a30ca1f0d1e8a4295aa6ca19196ee162
oai_identifier_str oai:upcommons.upc.edu:2117/396448
network_acronym_str ES
network_name_str España
repository_id_str
spelling Production of polyhydroxyalkanoates (PHAs) for use as biodegradable capsules in the pharmaceutical industryRodríguez Pájaro, JorgeBiodegradable plastics -- TestingBiopolymersPharmaceutical industryPoly(3-hydroxybutyrate-co-3-hydroxyvalerate)nanoparticlesbiodregradablepharmaceutical and medical industryPlàstics biodegradables -- ProvesBiopolímersIndústria farmacèuticaÀrees temàtiques de la UPC::Enginyeria químicaThe objective of this project has been the study of a biopolymer produced by microorganisms from waste, with biodegradability and biocompatibility properties, for use as a nanocapsule/nanoparticle in the pharmaceutical industry. The nanoparticles, made from Poly(3-hydroxybutyrate-co-3- hydroxyvalerate) (P(3HB-co-3HV)), a copolymer belonging to the family of polyhydroxyalkanoates (PHAs), have been obtained using the single emulsion/solvent evaporation technique. To produce the nanoparticles by emulsion, two immiscible phases are needed, in this case an organic phase, where chloroform (CHCl3) is used as a solvent for P(3HB-co-3HV), and an aqueous phase where Poly(vinyl alcohol) (PVA) is dissolved, which acts as a stabilizer/surfactant for the emulsion. Once the emulsion is obtained, the solvent is evaporated, and the nanoparticles are washed. Nanoparticles have been produced under conditions of different concentrations of polymer, surfactant, and speed of rotation of the homogenizer. The different groups of nanoparticles have been characterized by dynamic light scattering (DLS) and electrophoretic light scattering (ELS) to evaluate their mean hydrodynamic diameter and their polydispersity index (PDI), and their Z potential, respectively. In addition, a morphological characterization has been carried out using an optical microscope and scanning electron microscopy (SEM). The results of this analysis have been useful for the optimization of the production parameters of the nanoparticles, where it has been shown that the concentration of surfactant, and the speed of rotation have the most prominent role. Once the optimal conditions have been obtained, the functionalization or loading of the nanoparticles has been carried out with Triclosan, which is an antibacterial and antifungal agent, and with Ibuprofen, which is a nonsteroidal anti-inflammatory drug (NSAID) that is used to relieve pain, fever, and inflammation. The loaded nanoparticles have been subjected to tests to evaluate their encapsulation efficiency (EE), in addition to analyzing the release profile of both drugs in two media, a medium with a hydrophilic nature, phosphate buffered saline (1X PBS), and a medium of phosphate buffered saline supplemented with 15% absolute ethanol (1X PBS/15% Ethanol).Universitat Politècnica de CatalunyaPuiggalí Bellalta, JordiValle Mendoza, Luis Javier del20232023-07-0620232023-11-15master thesishttp://purl.org/coar/resource_type/c_bdccNAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/masterThesisapplication/pdfhttps://hdl.handle.net/2117/396448reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NoDerivs 4.0 Internationalhttp://creativecommons.org/licenses/by-nd/4.0/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3964482026-05-27T15:37:01Z
dc.title.none.fl_str_mv Production of polyhydroxyalkanoates (PHAs) for use as biodegradable capsules in the pharmaceutical industry
title Production of polyhydroxyalkanoates (PHAs) for use as biodegradable capsules in the pharmaceutical industry
spellingShingle Production of polyhydroxyalkanoates (PHAs) for use as biodegradable capsules in the pharmaceutical industry
Rodríguez Pájaro, Jorge
Biodegradable plastics -- Testing
Biopolymers
Pharmaceutical industry
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
nanoparticles
biodregradable
pharmaceutical and medical industry
Plàstics biodegradables -- Proves
Biopolímers
Indústria farmacèutica
Àrees temàtiques de la UPC::Enginyeria química
title_short Production of polyhydroxyalkanoates (PHAs) for use as biodegradable capsules in the pharmaceutical industry
title_full Production of polyhydroxyalkanoates (PHAs) for use as biodegradable capsules in the pharmaceutical industry
title_fullStr Production of polyhydroxyalkanoates (PHAs) for use as biodegradable capsules in the pharmaceutical industry
title_full_unstemmed Production of polyhydroxyalkanoates (PHAs) for use as biodegradable capsules in the pharmaceutical industry
title_sort Production of polyhydroxyalkanoates (PHAs) for use as biodegradable capsules in the pharmaceutical industry
dc.creator.none.fl_str_mv Rodríguez Pájaro, Jorge
author Rodríguez Pájaro, Jorge
author_facet Rodríguez Pájaro, Jorge
author_role author
dc.contributor.none.fl_str_mv Puiggalí Bellalta, Jordi
Valle Mendoza, Luis Javier del
dc.subject.none.fl_str_mv Biodegradable plastics -- Testing
Biopolymers
Pharmaceutical industry
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
nanoparticles
biodregradable
pharmaceutical and medical industry
Plàstics biodegradables -- Proves
Biopolímers
Indústria farmacèutica
Àrees temàtiques de la UPC::Enginyeria química
topic Biodegradable plastics -- Testing
Biopolymers
Pharmaceutical industry
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
nanoparticles
biodregradable
pharmaceutical and medical industry
Plàstics biodegradables -- Proves
Biopolímers
Indústria farmacèutica
Àrees temàtiques de la UPC::Enginyeria química
description The objective of this project has been the study of a biopolymer produced by microorganisms from waste, with biodegradability and biocompatibility properties, for use as a nanocapsule/nanoparticle in the pharmaceutical industry. The nanoparticles, made from Poly(3-hydroxybutyrate-co-3- hydroxyvalerate) (P(3HB-co-3HV)), a copolymer belonging to the family of polyhydroxyalkanoates (PHAs), have been obtained using the single emulsion/solvent evaporation technique. To produce the nanoparticles by emulsion, two immiscible phases are needed, in this case an organic phase, where chloroform (CHCl3) is used as a solvent for P(3HB-co-3HV), and an aqueous phase where Poly(vinyl alcohol) (PVA) is dissolved, which acts as a stabilizer/surfactant for the emulsion. Once the emulsion is obtained, the solvent is evaporated, and the nanoparticles are washed. Nanoparticles have been produced under conditions of different concentrations of polymer, surfactant, and speed of rotation of the homogenizer. The different groups of nanoparticles have been characterized by dynamic light scattering (DLS) and electrophoretic light scattering (ELS) to evaluate their mean hydrodynamic diameter and their polydispersity index (PDI), and their Z potential, respectively. In addition, a morphological characterization has been carried out using an optical microscope and scanning electron microscopy (SEM). The results of this analysis have been useful for the optimization of the production parameters of the nanoparticles, where it has been shown that the concentration of surfactant, and the speed of rotation have the most prominent role. Once the optimal conditions have been obtained, the functionalization or loading of the nanoparticles has been carried out with Triclosan, which is an antibacterial and antifungal agent, and with Ibuprofen, which is a nonsteroidal anti-inflammatory drug (NSAID) that is used to relieve pain, fever, and inflammation. The loaded nanoparticles have been subjected to tests to evaluate their encapsulation efficiency (EE), in addition to analyzing the release profile of both drugs in two media, a medium with a hydrophilic nature, phosphate buffered saline (1X PBS), and a medium of phosphate buffered saline supplemented with 15% absolute ethanol (1X PBS/15% Ethanol).
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-07-06
2023
2023-11-15
dc.type.none.fl_str_mv master thesis
http://purl.org/coar/resource_type/c_bdcc
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/396448
url https://hdl.handle.net/2117/396448
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NoDerivs 4.0 International
http://creativecommons.org/licenses/by-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NoDerivs 4.0 International
http://creativecommons.org/licenses/by-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universitat Politècnica de Catalunya
publisher.none.fl_str_mv Universitat Politècnica de Catalunya
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869415348317454336
score 15,300719

Similares en LA Referencia