Production of polyhydroxyalkanoates (PHAs) for use as biodegradable capsules in the pharmaceutical industry
The objective of this project has been the study of a biopolymer produced by microorganisms from waste, with biodegradability and biocompatibility properties, for use as a nanocapsule/nanoparticle in the pharmaceutical industry. The nanoparticles, made from Poly(3-hydroxybutyrate-co-3- hydroxyvalera...
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| Formato: | tesis de maestría |
| Fecha de publicación: | 2023 |
| País: | España |
| Recursos: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/396448 |
| Acesso em linha: | https://hdl.handle.net/2117/396448 |
| Access Level: | acceso abierto |
| Palavra-chave: | Biodegradable plastics -- Testing Biopolymers Pharmaceutical industry Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanoparticles biodregradable pharmaceutical and medical industry Plàstics biodegradables -- Proves Biopolímers Indústria farmacèutica Àrees temàtiques de la UPC::Enginyeria química |
| Resumo: | The objective of this project has been the study of a biopolymer produced by microorganisms from waste, with biodegradability and biocompatibility properties, for use as a nanocapsule/nanoparticle in the pharmaceutical industry. The nanoparticles, made from Poly(3-hydroxybutyrate-co-3- hydroxyvalerate) (P(3HB-co-3HV)), a copolymer belonging to the family of polyhydroxyalkanoates (PHAs), have been obtained using the single emulsion/solvent evaporation technique. To produce the nanoparticles by emulsion, two immiscible phases are needed, in this case an organic phase, where chloroform (CHCl3) is used as a solvent for P(3HB-co-3HV), and an aqueous phase where Poly(vinyl alcohol) (PVA) is dissolved, which acts as a stabilizer/surfactant for the emulsion. Once the emulsion is obtained, the solvent is evaporated, and the nanoparticles are washed. Nanoparticles have been produced under conditions of different concentrations of polymer, surfactant, and speed of rotation of the homogenizer. The different groups of nanoparticles have been characterized by dynamic light scattering (DLS) and electrophoretic light scattering (ELS) to evaluate their mean hydrodynamic diameter and their polydispersity index (PDI), and their Z potential, respectively. In addition, a morphological characterization has been carried out using an optical microscope and scanning electron microscopy (SEM). The results of this analysis have been useful for the optimization of the production parameters of the nanoparticles, where it has been shown that the concentration of surfactant, and the speed of rotation have the most prominent role. Once the optimal conditions have been obtained, the functionalization or loading of the nanoparticles has been carried out with Triclosan, which is an antibacterial and antifungal agent, and with Ibuprofen, which is a nonsteroidal anti-inflammatory drug (NSAID) that is used to relieve pain, fever, and inflammation. The loaded nanoparticles have been subjected to tests to evaluate their encapsulation efficiency (EE), in addition to analyzing the release profile of both drugs in two media, a medium with a hydrophilic nature, phosphate buffered saline (1X PBS), and a medium of phosphate buffered saline supplemented with 15% absolute ethanol (1X PBS/15% Ethanol). |
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