Interaction of antifungal peptide BP15 with Stemphylium vesicarium, the causal agent of brown spot of pear

Peptide BP15 has shown antifungal activity against several plant pathogenic fungi, including Stemphylium vesicarium, the causal agent of brown spot of pear. BP15 inhibits the germination, growth and sporulation of S. vesicarium and displays post-infection activity by stopping fungal infection in pea...

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Autores: Puig Garcia, Mireia, Moragrega i Garcia, Concepció, Ruz Estévez, Lídia, Calderón, Claudia E., Cazorla, Francisco M., Montesinos Seguí, Emilio, Llorente i Cabratosa, Isidre
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/12345
Acceso en línea:http://hdl.handle.net/10256/12345
Access Level:acceso embargado
Palabra clave:Fungicides
Perera -- Malalties i plagues
Pear -- Diseases and pests
Fongs en l'agricultura
Fungi in agriculture
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spelling Interaction of antifungal peptide BP15 with Stemphylium vesicarium, the causal agent of brown spot of pearPuig Garcia, MireiaMoragrega i Garcia, ConcepcióRuz Estévez, LídiaCalderón, Claudia E.Cazorla, Francisco M.Montesinos Seguí, EmilioLlorente i Cabratosa, IsidreFungicidesPerera -- Malalties i plaguesPear -- Diseases and pestsFongs en l'agriculturaFungi in agriculturePeptide BP15 has shown antifungal activity against several plant pathogenic fungi, including Stemphylium vesicarium, the causal agent of brown spot of pear. BP15 inhibits the germination, growth and sporulation of S. vesicarium and displays post-infection activity by stopping fungal infection in pear leaves. In this work, live-cell imaging was undertaken to understand the antifungal mechanism of BP15. A double-staining method based on the combination of calcofluor white and SYTOX green coupled with epifluorescence microscopy was used to investigate fungal cell permeabilization and alterations in fungal growth induced by BP15. GFP-transformants of S. vesicarium were obtained and exposed to rhodamine-labelled BP15. Confocal laser microscopy provided evidence of peptide internalization by hyphae, resulting in fungal cell disorganization and death. S. vesicarium membrane permeabilization by BP15 was found to be peptide-concentration dependent. BP15 at MIC and sub-MIC concentrations (10 and 5 μM, respectively) inhibited S. vesicarium growth and produced morphological alterations to germ tubes, with slow and discontinuous compromise of fungal cell membranes. Fungal cell membrane disruption was immediately induced by BP15 at 100 μM, and this was accompanied by rapid peptide internalization by S. vesicarium hyphae. Peptide BP15 interacted with germ tubes and hyphae of S. vesicarium but not with conidial cellsThis research was supported in part by grants from the Ministerio de Educaci on y Ciencia (AGL2009-09829/AGR and AGL2011-30354-C02-01) of SpainElsevierMinisterio de Ciencia e Innovación (Espanya)infoinfo2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10256/12345http://hdl.handle.net/10256/12345© Fungal Biology, 2016, vol. 120, núm. 1, p. 61-71Articles publicats (D-EQATA)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.funbio.2015.10.007info:eu-repo/semantics/altIdentifier/issn/1878-6146info:eu-repo/grantAgreement/MICINN//AGL2009-09829Tots els drets reservatsinfo:eu-repo/semantics/embargoedAccessoai:recercat.cat:10256/123452026-05-29T05:05:01Z
dc.title.none.fl_str_mv Interaction of antifungal peptide BP15 with Stemphylium vesicarium, the causal agent of brown spot of pear
title Interaction of antifungal peptide BP15 with Stemphylium vesicarium, the causal agent of brown spot of pear
spellingShingle Interaction of antifungal peptide BP15 with Stemphylium vesicarium, the causal agent of brown spot of pear
Puig Garcia, Mireia
Fungicides
Perera -- Malalties i plagues
Pear -- Diseases and pests
Fongs en l'agricultura
Fungi in agriculture
title_short Interaction of antifungal peptide BP15 with Stemphylium vesicarium, the causal agent of brown spot of pear
title_full Interaction of antifungal peptide BP15 with Stemphylium vesicarium, the causal agent of brown spot of pear
title_fullStr Interaction of antifungal peptide BP15 with Stemphylium vesicarium, the causal agent of brown spot of pear
title_full_unstemmed Interaction of antifungal peptide BP15 with Stemphylium vesicarium, the causal agent of brown spot of pear
title_sort Interaction of antifungal peptide BP15 with Stemphylium vesicarium, the causal agent of brown spot of pear
dc.creator.none.fl_str_mv Puig Garcia, Mireia
Moragrega i Garcia, Concepció
Ruz Estévez, Lídia
Calderón, Claudia E.
Cazorla, Francisco M.
Montesinos Seguí, Emilio
Llorente i Cabratosa, Isidre
author Puig Garcia, Mireia
author_facet Puig Garcia, Mireia
Moragrega i Garcia, Concepció
Ruz Estévez, Lídia
Calderón, Claudia E.
Cazorla, Francisco M.
Montesinos Seguí, Emilio
Llorente i Cabratosa, Isidre
author_role author
author2 Moragrega i Garcia, Concepció
Ruz Estévez, Lídia
Calderón, Claudia E.
Cazorla, Francisco M.
Montesinos Seguí, Emilio
Llorente i Cabratosa, Isidre
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (Espanya)
dc.subject.none.fl_str_mv Fungicides
Perera -- Malalties i plagues
Pear -- Diseases and pests
Fongs en l'agricultura
Fungi in agriculture
topic Fungicides
Perera -- Malalties i plagues
Pear -- Diseases and pests
Fongs en l'agricultura
Fungi in agriculture
description Peptide BP15 has shown antifungal activity against several plant pathogenic fungi, including Stemphylium vesicarium, the causal agent of brown spot of pear. BP15 inhibits the germination, growth and sporulation of S. vesicarium and displays post-infection activity by stopping fungal infection in pear leaves. In this work, live-cell imaging was undertaken to understand the antifungal mechanism of BP15. A double-staining method based on the combination of calcofluor white and SYTOX green coupled with epifluorescence microscopy was used to investigate fungal cell permeabilization and alterations in fungal growth induced by BP15. GFP-transformants of S. vesicarium were obtained and exposed to rhodamine-labelled BP15. Confocal laser microscopy provided evidence of peptide internalization by hyphae, resulting in fungal cell disorganization and death. S. vesicarium membrane permeabilization by BP15 was found to be peptide-concentration dependent. BP15 at MIC and sub-MIC concentrations (10 and 5 μM, respectively) inhibited S. vesicarium growth and produced morphological alterations to germ tubes, with slow and discontinuous compromise of fungal cell membranes. Fungal cell membrane disruption was immediately induced by BP15 at 100 μM, and this was accompanied by rapid peptide internalization by S. vesicarium hyphae. Peptide BP15 interacted with germ tubes and hyphae of S. vesicarium but not with conidial cells
publishDate 2016
dc.date.none.fl_str_mv 2016
info
info
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/12345
http://hdl.handle.net/10256/12345
url http://hdl.handle.net/10256/12345
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.funbio.2015.10.007
info:eu-repo/semantics/altIdentifier/issn/1878-6146
info:eu-repo/grantAgreement/MICINN//AGL2009-09829
dc.rights.none.fl_str_mv Tots els drets reservats
info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv Tots els drets reservats
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv © Fungal Biology, 2016, vol. 120, núm. 1, p. 61-71
Articles publicats (D-EQATA)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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