Synthetic antifungal peptides for controlling brown spot of pear caused by Stemphylium vesicarium. Activity, mode of action and field evaluation
Brown spot of pear is a fungal disease caused by Stemphylium vesicarium. Currently, the disease control efficacy is limited, due to the characteristics of disease and the reduction of the allowed fungicides. Therefore, this thesis is focused on finding new compounds to be applied alternatively or as...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/387564 |
| Acceso en línea: | http://hdl.handle.net/10803/387564 |
| Access Level: | acceso abierto |
| Palabra clave: | Antimicrobial peptides Pèptids antimicrobians Péptidos antimicrobianos Perera Pear Peral Brown spot Estemfiliosi Estemfiliosis Stemphylium vesicarium Fungicides Fungicidas Integrated pest management Gestió de plagues integral Gestión integral de plagas 630 631/635 |
| Sumario: | Brown spot of pear is a fungal disease caused by Stemphylium vesicarium. Currently, the disease control efficacy is limited, due to the characteristics of disease and the reduction of the allowed fungicides. Therefore, this thesis is focused on finding new compounds to be applied alternatively or as a complement to the existing fungicides. Thus, twelve synthetic antifungal peptides selected from CECMEL11 library were evaluated in vitro against S. vesicarium. The most effective peptide was BP15 with a minimum inhibitory concentration (MIC) of 10 µM. Moreover, the peptide reduced significantly the disease severity in both ex vivo assays and under field conditions. The mechanisms of action of BP15 were studied. It was determined that the peptide permeabilizes the fungal cell membrane and it is internalized by the cells of germ tubes and hyphae of S. vesicarium. |
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