Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2

Mouse and human fibroblasts were the first cell types successfully reprog- rammed by ectopic expression of OCT4, SOX2, KLF4, and c-MYC (OSKM) (Lowry et al., 2008; Maherali et al., 2007; Park et al., 2008; Takahashi et al., 2007; Taka- hashi and Yamanaka, 2006; Yu et al., 2007). Further studies have...

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Autores: Giorgetti, Alessandra, Montserrat, Núria, Aasen, Trond, Gonzalez, Federico, Rodríguez-Pizà, Ignacio, Vassena, Rita, Raya Chamorro, Ángel, Boué, Stefanie, Barrero, Maria J., Aran Corbella, Begoña, Torrabadella, Marta, Veiga, Anna, Izpisúa Belmonte, Juan Carlos
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2009
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/176410
Acceso en línea:https://hdl.handle.net/2445/176410
Access Level:acceso abierto
Palabra clave:Cèl·lules mare
Cordó umbilical
Citologia
Stem cells
Umbilical cord
Cytology
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spelling Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2Giorgetti, AlessandraMontserrat, NúriaAasen, TrondGonzalez, FedericoRodríguez-Pizà, IgnacioVassena, RitaRaya Chamorro, ÁngelBoué, StefanieBarrero, Maria J.Aran Corbella, BegoñaTorrabadella, MartaVeiga, AnnaIzpisúa Belmonte, Juan CarlosCèl·lules mareCordó umbilicalCitologiaStem cellsUmbilical cordCytologyMouse and human fibroblasts were the first cell types successfully reprog- rammed by ectopic expression of OCT4, SOX2, KLF4, and c-MYC (OSKM) (Lowry et al., 2008; Maherali et al., 2007; Park et al., 2008; Takahashi et al., 2007; Taka- hashi and Yamanaka, 2006; Yu et al., 2007). Further studies have shown that the age, origin, and cell type used have a deep impact on the reprogramming effi- ciency, eventually requiring the expres- sion of fewer factors and/or reducing the timing of the whole process. In general, stem cells are rare and difficult to access and isolate in large numbers (neural stem cells, for instance [Kim et al., 2008, 2009c]) and, therefore, represent a com- plicated target for reprogramming. How- ever, Cord Blood (CB) could represent an alternative and readily accessible source of stem cells. Here, we describe reprog- ramming of CB cells to pluripotency by retroviral transduction of four (OSKM), three (OSK), and as few as two (OS) tran- scription factors, without the need for additional chemical compounds.Elsevier B.V.2021202120092021info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion10 p.application/pdfhttps://hdl.handle.net/2445/176410Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1016/j.stem.2009.09.008Cell Stem Cell, 2009, vol. 5, num. 4, p. 353-357https://doi.org/10.1016/j.stem.2009.09.008(c) Elsevier B.V., 2009info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1764102026-05-29T05:05:01Z
dc.title.none.fl_str_mv Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2
title Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2
spellingShingle Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2
Giorgetti, Alessandra
Cèl·lules mare
Cordó umbilical
Citologia
Stem cells
Umbilical cord
Cytology
title_short Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2
title_full Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2
title_fullStr Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2
title_full_unstemmed Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2
title_sort Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2
dc.creator.none.fl_str_mv Giorgetti, Alessandra
Montserrat, Núria
Aasen, Trond
Gonzalez, Federico
Rodríguez-Pizà, Ignacio
Vassena, Rita
Raya Chamorro, Ángel
Boué, Stefanie
Barrero, Maria J.
Aran Corbella, Begoña
Torrabadella, Marta
Veiga, Anna
Izpisúa Belmonte, Juan Carlos
author Giorgetti, Alessandra
author_facet Giorgetti, Alessandra
Montserrat, Núria
Aasen, Trond
Gonzalez, Federico
Rodríguez-Pizà, Ignacio
Vassena, Rita
Raya Chamorro, Ángel
Boué, Stefanie
Barrero, Maria J.
Aran Corbella, Begoña
Torrabadella, Marta
Veiga, Anna
Izpisúa Belmonte, Juan Carlos
author_role author
author2 Montserrat, Núria
Aasen, Trond
Gonzalez, Federico
Rodríguez-Pizà, Ignacio
Vassena, Rita
Raya Chamorro, Ángel
Boué, Stefanie
Barrero, Maria J.
Aran Corbella, Begoña
Torrabadella, Marta
Veiga, Anna
Izpisúa Belmonte, Juan Carlos
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cèl·lules mare
Cordó umbilical
Citologia
Stem cells
Umbilical cord
Cytology
topic Cèl·lules mare
Cordó umbilical
Citologia
Stem cells
Umbilical cord
Cytology
description Mouse and human fibroblasts were the first cell types successfully reprog- rammed by ectopic expression of OCT4, SOX2, KLF4, and c-MYC (OSKM) (Lowry et al., 2008; Maherali et al., 2007; Park et al., 2008; Takahashi et al., 2007; Taka- hashi and Yamanaka, 2006; Yu et al., 2007). Further studies have shown that the age, origin, and cell type used have a deep impact on the reprogramming effi- ciency, eventually requiring the expres- sion of fewer factors and/or reducing the timing of the whole process. In general, stem cells are rare and difficult to access and isolate in large numbers (neural stem cells, for instance [Kim et al., 2008, 2009c]) and, therefore, represent a com- plicated target for reprogramming. How- ever, Cord Blood (CB) could represent an alternative and readily accessible source of stem cells. Here, we describe reprog- ramming of CB cells to pluripotency by retroviral transduction of four (OSKM), three (OSK), and as few as two (OS) tran- scription factors, without the need for additional chemical compounds.
publishDate 2009
dc.date.none.fl_str_mv 2009
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176410
url https://hdl.handle.net/2445/176410
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.stem.2009.09.008
Cell Stem Cell, 2009, vol. 5, num. 4, p. 353-357
https://doi.org/10.1016/j.stem.2009.09.008
dc.rights.none.fl_str_mv (c) Elsevier B.V., 2009
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Elsevier B.V., 2009
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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