Evaluation of the Spanish population coverage of a prospective HLA haplobank of induced pluripotent stem cells

Background: iPSC (induced pluripotent stem cells) banks of iPSC lines with homozygous HLA (human leukocyte antigen) haplotypes (haplobanks) are proposed as an affordable and off-the-shelf approach to allogeneic transplantation of iPSC derived cell therapies. Cord blood banks offer an extensive sourc...

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Detalles Bibliográficos
Autores: Álvarez Palomo, Belén, García Martínez, Iris, Gayoso, Jorge, Raya Chamorro, Ángel, Veiga, Anna, Abad, María Lluisa, Eiras, Adolfo, Guzmán Fulgencio, María, Luis Hidalgo, Mar, Eguizábal, Cristina, Santos, Silvia, Balas, Antonio, Alenda, Raquel, Sánchez Gordo, Francisco, Ponce Verdugo, Laura, Villa, Juliana, Carreras, Enric, Vidal, Francisco, Madrigal, Alejandro, Herrero, María José, Rudilla Salvador, Francesc, Querol Giner, Sergi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/176988
Acceso en línea:https://hdl.handle.net/2445/176988
Access Level:acceso abierto
Palabra clave:Cèl·lules mare
Cordó umbilical
Stem cells
Umbilical cord
Descripción
Sumario:Background: iPSC (induced pluripotent stem cells) banks of iPSC lines with homozygous HLA (human leukocyte antigen) haplotypes (haplobanks) are proposed as an affordable and off-the-shelf approach to allogeneic transplantation of iPSC derived cell therapies. Cord blood banks offer an extensive source of HLA-typed cells suitable for reprogramming to iPSC. Several initiatives worldwide have been undertaken to create national and international iPSC haplobanks that match a significant part of a population. Methods: To create an iPSC haplobank that serves the Spanish population (IPS-PANIA), we have searched the Spanish Bone Marrow Donor Registry (REDMO) to identify the most frequently estimated haplotypes. From the top ten donors identified, we estimated the population coverage using the criteria of zero mismatches in HLA-A, HLA-B, and HLA-DRB1 with different stringencies: high resolution, low resolution, and beneficial mismatch. Results: We have calculated that ten cord blood units from homozygous donors stored at the Spanish cord blood banks can provide HLA-A, HLA-B, and HLA-DRB1 matching for 28.23% of the population. Conclusion: We confirm the feasibility of using banked cord blood units to create an iPSC haplobank that will cover a significant percentage of the Spanish and international population for future advanced therapy replacement strategies.

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