Xylitol-fed and control microbiota DataSet

[Description of methods used for collection/generation of data] Pooled-fecal microbiota from 5-7 years old chlidren was inoculated into the three colon reactors (R1, R2 and R3) of the BFBL gut simulator. The stabilized microbiota were inoculated (1%) with the child pooled fecal sample. After the mic...

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Detalhes bibliográficos
Autores: Bellanco-Sevilla, Alicia, Celcar, Špela, Martínez-Cuesta, M. Carmen, Requena, Teresa
Formato: conjunto de datos
Fecha de publicación:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/348283
Acesso em linha:http://hdl.handle.net/10261/348283
https://doi.org/10.20350/digitalCSIC/16138
Access Level:acceso abierto
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repository_id_str
spelling Xylitol-fed and control microbiota DataSetBellanco-Sevilla, AliciaCelcar, ŠpelaMartínez-Cuesta, M. CarmenRequena, Teresa[Description of methods used for collection/generation of data] Pooled-fecal microbiota from 5-7 years old chlidren was inoculated into the three colon reactors (R1, R2 and R3) of the BFBL gut simulator. The stabilized microbiota were inoculated (1%) with the child pooled fecal sample. After the microbiota stabilization stage, xylitol was added to the system at increasing concentrations (1 g/L, 3 g/L and 5 g/L), three times per day for 7 days for each concentration (samples X). The experiment without addition of xylitol to the BFBL gut simulator was carried out for the same period of time (Control, samples C). During the experimental set up, samples were collected every 24 h from the three colon reactors and centrifuged (11,200 ×g during 10 min at 4 °C), and the pellet was used for bacterial genomic DNA extraction (EZNA® Bacterial DNA Kit, Omega Biotek) and using a FastPrep equipment for mechanical lysis (Bio 101 FastPrep FP120, Savant Instruments). DNA samples were sent to Novogene Europe (Cambridge, UK) for sequencing the V3-V4 region of the 16S rRNA gene amplified by using 341F (5′- CCTAYGGGRBGCASCAG-3′) and 806R (5′-GGA CTACNNGGGTATCTAAT-3′) primers. The PCR products were sequenced on a paired-end Illumina platform (NovaSeq 6000, Illumina) to generate 250 bp paired-end raw reads.MCIN/ AEI /10.13039/501100011033 (project reference: PID2019- 382 106071RB-I00).Peer reviewedDIGITAL.CSICMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Requena, Teresa [0000-0002-8846-6846]Martínez-Cuesta, M. Carmen [0000-0001-9618-2571]Requena, Teresa [t.requena@csic.es]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1http://hdl.handle.net/10261/348283https://doi.org/10.20350/digitalCSIC/16138reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//PID2019-382106071RB-I00Bellanco-Sevilla, Alicia; Celcar, S.; Martínez-Cuesta, M. Carmen; Requena, Teresa. The food additive xylitol enhances the butyrate formation by the child gut microbiota developed in a dynamic colonic simulator. https://doi.org/10.1016/j.fct.2024.114605. http://hdl.handle.net/10261/364356Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3482832026-05-22T06:33:51Z
dc.title.none.fl_str_mv Xylitol-fed and control microbiota DataSet
title Xylitol-fed and control microbiota DataSet
spellingShingle Xylitol-fed and control microbiota DataSet
Bellanco-Sevilla, Alicia
title_short Xylitol-fed and control microbiota DataSet
title_full Xylitol-fed and control microbiota DataSet
title_fullStr Xylitol-fed and control microbiota DataSet
title_full_unstemmed Xylitol-fed and control microbiota DataSet
title_sort Xylitol-fed and control microbiota DataSet
dc.creator.none.fl_str_mv Bellanco-Sevilla, Alicia
Celcar, Špela
Martínez-Cuesta, M. Carmen
Requena, Teresa
author Bellanco-Sevilla, Alicia
author_facet Bellanco-Sevilla, Alicia
Celcar, Špela
Martínez-Cuesta, M. Carmen
Requena, Teresa
author_role author
author2 Celcar, Špela
Martínez-Cuesta, M. Carmen
Requena, Teresa
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Requena, Teresa [0000-0002-8846-6846]
Martínez-Cuesta, M. Carmen [0000-0001-9618-2571]
Requena, Teresa [t.requena@csic.es]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description [Description of methods used for collection/generation of data] Pooled-fecal microbiota from 5-7 years old chlidren was inoculated into the three colon reactors (R1, R2 and R3) of the BFBL gut simulator. The stabilized microbiota were inoculated (1%) with the child pooled fecal sample. After the microbiota stabilization stage, xylitol was added to the system at increasing concentrations (1 g/L, 3 g/L and 5 g/L), three times per day for 7 days for each concentration (samples X). The experiment without addition of xylitol to the BFBL gut simulator was carried out for the same period of time (Control, samples C). During the experimental set up, samples were collected every 24 h from the three colon reactors and centrifuged (11,200 ×g during 10 min at 4 °C), and the pellet was used for bacterial genomic DNA extraction (EZNA® Bacterial DNA Kit, Omega Biotek) and using a FastPrep equipment for mechanical lysis (Bio 101 FastPrep FP120, Savant Instruments). DNA samples were sent to Novogene Europe (Cambridge, UK) for sequencing the V3-V4 region of the 16S rRNA gene amplified by using 341F (5′- CCTAYGGGRBGCASCAG-3′) and 806R (5′-GGA CTACNNGGGTATCTAAT-3′) primers. The PCR products were sequenced on a paired-end Illumina platform (NovaSeq 6000, Illumina) to generate 250 bp paired-end raw reads.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
format dataset
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/348283
https://doi.org/10.20350/digitalCSIC/16138
url http://hdl.handle.net/10261/348283
https://doi.org/10.20350/digitalCSIC/16138
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI//PID2019-382106071RB-I00
Bellanco-Sevilla, Alicia; Celcar, S.; Martínez-Cuesta, M. Carmen; Requena, Teresa. The food additive xylitol enhances the butyrate formation by the child gut microbiota developed in a dynamic colonic simulator. https://doi.org/10.1016/j.fct.2024.114605. http://hdl.handle.net/10261/364356
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv DIGITAL.CSIC
publisher.none.fl_str_mv DIGITAL.CSIC
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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