Xylitol-fed and control microbiota DataSet
[Description of methods used for collection/generation of data] Pooled-fecal microbiota from 5-7 years old chlidren was inoculated into the three colon reactors (R1, R2 and R3) of the BFBL gut simulator. The stabilized microbiota were inoculated (1%) with the child pooled fecal sample. After the mic...
| Autores: | , , , |
|---|---|
| Tipo de recurso: | conjunto de datos |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/348283 |
| Acceso en línea: | http://hdl.handle.net/10261/348283 https://doi.org/10.20350/digitalCSIC/16138 |
| Access Level: | acceso abierto |
| Sumario: | [Description of methods used for collection/generation of data] Pooled-fecal microbiota from 5-7 years old chlidren was inoculated into the three colon reactors (R1, R2 and R3) of the BFBL gut simulator. The stabilized microbiota were inoculated (1%) with the child pooled fecal sample. After the microbiota stabilization stage, xylitol was added to the system at increasing concentrations (1 g/L, 3 g/L and 5 g/L), three times per day for 7 days for each concentration (samples X). The experiment without addition of xylitol to the BFBL gut simulator was carried out for the same period of time (Control, samples C). During the experimental set up, samples were collected every 24 h from the three colon reactors and centrifuged (11,200 ×g during 10 min at 4 °C), and the pellet was used for bacterial genomic DNA extraction (EZNA® Bacterial DNA Kit, Omega Biotek) and using a FastPrep equipment for mechanical lysis (Bio 101 FastPrep FP120, Savant Instruments). DNA samples were sent to Novogene Europe (Cambridge, UK) for sequencing the V3-V4 region of the 16S rRNA gene amplified by using 341F (5′- CCTAYGGGRBGCASCAG-3′) and 806R (5′-GGA CTACNNGGGTATCTAAT-3′) primers. The PCR products were sequenced on a paired-end Illumina platform (NovaSeq 6000, Illumina) to generate 250 bp paired-end raw reads. |
|---|