Ecotoxicity of Thymol in Dung Beetles (Coleoptera: Geotrupidae) and Its Potential as a Safer Anthelmintic Alternative [Dataset]

Dung beetles play a crucial role in rangeland sustainability by facilitating dung removal, nutrient recycling, soil bioturbation, parasite suppression, and greenhouse gas mitigation. However, the widespread prophylactic use of synthetic anthelmintics, particularly macrocyclic lactones such as iverme...

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Detalles Bibliográficos
Autores: Cortez, Vieyle, Verdú, José R., González-Rodríguez, Estela, Ortiz, Antonio J., Rosa-García, Rocío, Cortez, Vieyle|||/items/b706ed6b-3ac0-4743-8fcf-380a0da522b6, Verdú, José R.|||/items/aec43e86-8ee7-42d2-825d-88e30c9e56c5
Tipo de recurso: conjunto de datos
Fecha de publicación:2026
País:España
Institución:Universidad de Alicante (UA)
Repositorio:RUA. Repositorio Institucional de la Universidad de Alicante
Idioma:inglés
OAI Identifier:oai:dnet:ruarepositor::00e2b286f1f2915998a8f718724e0a2c
Acceso en línea:https://hdl.handle.net/10045/161910
Access Level:acceso abierto
Palabra clave:Dung beetles
Thymol
Ecotoxicity
Electroantennography
Phenoloxidase
Macrocyclic lactones
Sustainable livestock management
Descripción
Sumario:Dung beetles play a crucial role in rangeland sustainability by facilitating dung removal, nutrient recycling, soil bioturbation, parasite suppression, and greenhouse gas mitigation. However, the widespread prophylactic use of synthetic anthelmintics, particularly macrocyclic lactones such as ivermectin, results in the excretion of biologically active residues in faeces that cause lethal and sublethal effects on non-target coprophagous insects. These ecotoxicological impacts threaten dung beetle diversity and compromise ecosystem functioning in pastoral landscapes. In contrast, plant-derived anthelmintics such as thymol (THY) are emerging as more environmentally compatible alternatives. In this study, we evaluated the sublethal ecotoxicity of dietary THY exposure in the dung beetle Thorectes lusitanicus Jeckel, 1866 (Coleoptera: Geotrupidae), a flightless telephagic tunneller native to Iberian Mediterranean ecosystems. Antennal sensory performance was quantified using electroantennography (EAG), while immunocompetence was assessed through haemolymph total protein content, phenoloxidase (PO) activity and prophenoloxidase (proPO) levels. Ivermectin was included as a reference treatment due to its known disruptive effects on insect olfactory and immune systems. THY ingestion did not impair antennal responsiveness or modify immune parameters relative to controls, even at the highest exposure concentrations tested. In contrast, ivermectin significantly reduced olfactory sensitivity and induced measurable immune alterations. Collectively, these findings support THY as a promising phytochemical anthelmintic with minimal ecotoxic risk to dung beetles. Its incorporation into livestock parasite-control strategies may reduce the environmental footprint of veterinary pharmaceuticals and help maintain the essential ecosystem services provided by dung-associated fauna.