Genetic and species rearrangements in microbial consortia impact biodegradation potential

Genomic reorganisation between species and horizontal gene transfer have been considered the most important mechanism of biological adaptation under selective pressure. Still, the impact of mobile genes in microbial ecology is far from being completely understood. Here we present the collection and...

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Autores: Saati-Santamaría, Zaki, Navarro-Gómez, Pilar, Martínez-Mancebo, Juan A., Juárez-Mugarza, Maitane, Flores, Amando, Canosa, Inés
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/394411
Acceso en línea:http://hdl.handle.net/10261/394411
Access Level:acceso abierto
Palabra clave:Ibuprofen
Microbial ecology
Consortia evolution
Biodegradation
Emerging pollutants
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spelling Genetic and species rearrangements in microbial consortia impact biodegradation potentialSaati-Santamaría, ZakiNavarro-Gómez, PilarMartínez-Mancebo, Juan A.Juárez-Mugarza, MaitaneFlores, AmandoCanosa, InésIbuprofenMicrobial ecologyConsortia evolutionBiodegradationEmerging pollutantsGenomic reorganisation between species and horizontal gene transfer have been considered the most important mechanism of biological adaptation under selective pressure. Still, the impact of mobile genes in microbial ecology is far from being completely understood. Here we present the collection and characterisation of microbial consortia enriched from environments contaminated with emerging pollutants, such as non-steroidal anti-inflammatory drugs. We have obtained and further enriched two ibuprofen-degrading microbial consortia from two unrelated wastewater treatment plants. We have also studied their ability to degrade the drug and the dynamics of the re-organisations of the genetic information responsible for its biodegradation among the species within the consortium. Our results show that genomic reorganisation within microorganisms and species rearrangements occur rapidly and efficiently during the selection process, which may be facilitated by plasmids and/or transposable elements located within the sequences. We show the evolution of at least two different plasmid backbones on samples from different locations, showing rearrangements of genomic information, including genes encoding activities for IBU degradation. As a result, we found variations in the expression pattern of the consortia after evolution under selective pressure, as an adaptation process to the new conditions. This work provides evidence for changes in the metagenomes of microbial communities that allow adaptation under a selective constraint –ibuprofen as a sole carbon source– and represents a step forward in knowledge that can inspire future biotechnological developments for drug bioremediation.PNG is recipient of the Margarita Salas Program Grant by the University of Seville from the Ministry of Universities. ZSS received funding from the Regional Government of Castilla y León (Escalera de Excelencia CLU-2018-04, co-funded by P.O. FEDER 2014–2020), the European NextGenerationEU program (Plan de Recuperación, Transformación y Resiliencia, Spanish Ministry of Universities), the University of Salamanca (“Ayudas para la recualificación del sistema universitario español 2021-2022”), and the EU Horizon Europe program (HORIZON-TMA-MSCA-PF-EF, Grant No. 101090267). This work has been funded by the Programa de Excelencia de la Junta de Andalucía (ProyExcel_00358) granted to IC and AF, and the V Plan Propio de investigación of the University Pablo de Olavide.Peer reviewedOxford University PressUniversidad de SevillaMinisterio de Universidades (España)Junta de Castilla y LeónEuropean CommissionUniversidad de SalamancaEuropean CommissionJunta de AndalucíaUniversidad Pablo de OlavideConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/394411reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/HE/101090267The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1093/ismejo/wraf014https://doi.org/10.1093/ismejo/wraf014Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3944112026-05-22T06:33:51Z
dc.title.none.fl_str_mv Genetic and species rearrangements in microbial consortia impact biodegradation potential
title Genetic and species rearrangements in microbial consortia impact biodegradation potential
spellingShingle Genetic and species rearrangements in microbial consortia impact biodegradation potential
Saati-Santamaría, Zaki
Ibuprofen
Microbial ecology
Consortia evolution
Biodegradation
Emerging pollutants
title_short Genetic and species rearrangements in microbial consortia impact biodegradation potential
title_full Genetic and species rearrangements in microbial consortia impact biodegradation potential
title_fullStr Genetic and species rearrangements in microbial consortia impact biodegradation potential
title_full_unstemmed Genetic and species rearrangements in microbial consortia impact biodegradation potential
title_sort Genetic and species rearrangements in microbial consortia impact biodegradation potential
dc.creator.none.fl_str_mv Saati-Santamaría, Zaki
Navarro-Gómez, Pilar
Martínez-Mancebo, Juan A.
Juárez-Mugarza, Maitane
Flores, Amando
Canosa, Inés
author Saati-Santamaría, Zaki
author_facet Saati-Santamaría, Zaki
Navarro-Gómez, Pilar
Martínez-Mancebo, Juan A.
Juárez-Mugarza, Maitane
Flores, Amando
Canosa, Inés
author_role author
author2 Navarro-Gómez, Pilar
Martínez-Mancebo, Juan A.
Juárez-Mugarza, Maitane
Flores, Amando
Canosa, Inés
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad de Sevilla
Ministerio de Universidades (España)
Junta de Castilla y León
European Commission
Universidad de Salamanca
European Commission
Junta de Andalucía
Universidad Pablo de Olavide
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Ibuprofen
Microbial ecology
Consortia evolution
Biodegradation
Emerging pollutants
topic Ibuprofen
Microbial ecology
Consortia evolution
Biodegradation
Emerging pollutants
description Genomic reorganisation between species and horizontal gene transfer have been considered the most important mechanism of biological adaptation under selective pressure. Still, the impact of mobile genes in microbial ecology is far from being completely understood. Here we present the collection and characterisation of microbial consortia enriched from environments contaminated with emerging pollutants, such as non-steroidal anti-inflammatory drugs. We have obtained and further enriched two ibuprofen-degrading microbial consortia from two unrelated wastewater treatment plants. We have also studied their ability to degrade the drug and the dynamics of the re-organisations of the genetic information responsible for its biodegradation among the species within the consortium. Our results show that genomic reorganisation within microorganisms and species rearrangements occur rapidly and efficiently during the selection process, which may be facilitated by plasmids and/or transposable elements located within the sequences. We show the evolution of at least two different plasmid backbones on samples from different locations, showing rearrangements of genomic information, including genes encoding activities for IBU degradation. As a result, we found variations in the expression pattern of the consortia after evolution under selective pressure, as an adaptation process to the new conditions. This work provides evidence for changes in the metagenomes of microbial communities that allow adaptation under a selective constraint –ibuprofen as a sole carbon source– and represents a step forward in knowledge that can inspire future biotechnological developments for drug bioremediation.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/394411
url http://hdl.handle.net/10261/394411
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/HE/101090267
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1093/ismejo/wraf014
https://doi.org/10.1093/ismejo/wraf014
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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