LAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patients

Background: Microscopy and rapid diagnosis tests have a limited sensitivity in diagnosis of malaria by Plasmodium ovale. The LAMP kit (LoopAMP®) can be used in the field without special equipment and could have an important role in malaria control programmes in endemic areas and for malaria diagnosi...

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Autores: Cuadros González, Juan|||0000-0003-4098-4377, Martin Ramírez, Alexandra, González, Iveth J., Ding, Xavier C., Pérez Tanoira, Ramón|||0000-0002-9816-3208, Rojo Marcos, Gerardo, Gómez Herruz, Peña, Rubio Muñoz, José Miguel
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/60470
Acceso en línea:http://hdl.handle.net/10017/60470
https://dx.doi.org/10.1186/s12936-016-1669-8
Access Level:acceso abierto
Palabra clave:LAMP
Loop-mediated isothermal amplification
Malaria
Plasmodium ovale
Medicina
Medicine
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spelling LAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patientsCuadros González, Juan|||0000-0003-4098-4377Martin Ramírez, AlexandraGonzález, Iveth J.Ding, Xavier C.Pérez Tanoira, Ramón|||0000-0002-9816-3208Rojo Marcos, GerardoGómez Herruz, PeñaRubio Muñoz, José MiguelLAMPLoop-mediated isothermal amplificationMalariaPlasmodium ovaleMedicinaMedicineBackground: Microscopy and rapid diagnosis tests have a limited sensitivity in diagnosis of malaria by Plasmodium ovale. The LAMP kit (LoopAMP®) can be used in the field without special equipment and could have an important role in malaria control programmes in endemic areas and for malaria diagnosis in returned travellers. The performance of the Pan primer of the kit in detecting malaria by P. ovale was compared with the results of standard nPCR in samples of patients returning from P. ovale endemic areas. Methods: Plasmodium ovale positive samples (29, tested by PCR and/or microscopy) and malaria negative specimens (398, tested by microscopy and PCR) were collected in different hospitals of Europe from June 2014 to March 2016 and frozen at -20 °C. Boil and spin method was used to extract DNA from all samples and amplification was performed with LoopAMP® MALARIA kit (Eiken Chemical, Japan) in an automated turbidimeter (Eiken 500). The results of LAMP read by turbidimetry and with the naked eye were compared. Results: The kit showed a sensitivity of 100% and a specificity of 97.24% with positive and negative predictive values of 72.5 and 100%, respectively. Naked eyed readings were in accordance with turbidimetry readings (sensitivity, 92.5%, specificity, 98.96% and positive and negative predictive values, respectively, 90.24 and 99.22%). The limit of detection of LAMP assay for P. ovale was between 0.8 and 2 parasites/µl. Conclusions: The Pan primer of the Malaria kit LoopAMP® can detect P. ovale at very low-levels and showed a predictive negative value of 100%. This tool can be useful in malaria control and elimination programmes and in returned travellers from P. ovale endemic areas. Naked eye readings are equivalent to automated turbidimeter readings in specimens obtained with EDTA.20172017-01-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/60470https://dx.doi.org/10.1186/s12936-016-1669-8reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/604702026-06-18T11:13:07Z
dc.title.none.fl_str_mv LAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patients
title LAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patients
spellingShingle LAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patients
Cuadros González, Juan|||0000-0003-4098-4377
LAMP
Loop-mediated isothermal amplification
Malaria
Plasmodium ovale
Medicina
Medicine
title_short LAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patients
title_full LAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patients
title_fullStr LAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patients
title_full_unstemmed LAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patients
title_sort LAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patients
dc.creator.none.fl_str_mv Cuadros González, Juan|||0000-0003-4098-4377
Martin Ramírez, Alexandra
González, Iveth J.
Ding, Xavier C.
Pérez Tanoira, Ramón|||0000-0002-9816-3208
Rojo Marcos, Gerardo
Gómez Herruz, Peña
Rubio Muñoz, José Miguel
author Cuadros González, Juan|||0000-0003-4098-4377
author_facet Cuadros González, Juan|||0000-0003-4098-4377
Martin Ramírez, Alexandra
González, Iveth J.
Ding, Xavier C.
Pérez Tanoira, Ramón|||0000-0002-9816-3208
Rojo Marcos, Gerardo
Gómez Herruz, Peña
Rubio Muñoz, José Miguel
author_role author
author2 Martin Ramírez, Alexandra
González, Iveth J.
Ding, Xavier C.
Pérez Tanoira, Ramón|||0000-0002-9816-3208
Rojo Marcos, Gerardo
Gómez Herruz, Peña
Rubio Muñoz, José Miguel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LAMP
Loop-mediated isothermal amplification
Malaria
Plasmodium ovale
Medicina
Medicine
topic LAMP
Loop-mediated isothermal amplification
Malaria
Plasmodium ovale
Medicina
Medicine
description Background: Microscopy and rapid diagnosis tests have a limited sensitivity in diagnosis of malaria by Plasmodium ovale. The LAMP kit (LoopAMP®) can be used in the field without special equipment and could have an important role in malaria control programmes in endemic areas and for malaria diagnosis in returned travellers. The performance of the Pan primer of the kit in detecting malaria by P. ovale was compared with the results of standard nPCR in samples of patients returning from P. ovale endemic areas. Methods: Plasmodium ovale positive samples (29, tested by PCR and/or microscopy) and malaria negative specimens (398, tested by microscopy and PCR) were collected in different hospitals of Europe from June 2014 to March 2016 and frozen at -20 °C. Boil and spin method was used to extract DNA from all samples and amplification was performed with LoopAMP® MALARIA kit (Eiken Chemical, Japan) in an automated turbidimeter (Eiken 500). The results of LAMP read by turbidimetry and with the naked eye were compared. Results: The kit showed a sensitivity of 100% and a specificity of 97.24% with positive and negative predictive values of 72.5 and 100%, respectively. Naked eyed readings were in accordance with turbidimetry readings (sensitivity, 92.5%, specificity, 98.96% and positive and negative predictive values, respectively, 90.24 and 99.22%). The limit of detection of LAMP assay for P. ovale was between 0.8 and 2 parasites/µl. Conclusions: The Pan primer of the Malaria kit LoopAMP® can detect P. ovale at very low-levels and showed a predictive negative value of 100%. This tool can be useful in malaria control and elimination programmes and in returned travellers from P. ovale endemic areas. Naked eye readings are equivalent to automated turbidimeter readings in specimens obtained with EDTA.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/60470
https://dx.doi.org/10.1186/s12936-016-1669-8
url http://hdl.handle.net/10017/60470
https://dx.doi.org/10.1186/s12936-016-1669-8
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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