First field and laboratory evaluation of LAMP assay for malaria diagnosis in Cubal, Angola
[EN]Malaria is a globally distributed infectious disease. According to the World Health Organization, Angola is one of the six countries that account for over half the global malaria burden in terms of both malaria cases and deaths. Diagnosis of malaria still depends on microscopic examination of th...
| Autores: | , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universidad de Salamanca (USAL) |
| Repositorio: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/155112 |
| Acceso en línea: | http://hdl.handle.net/10366/155112 |
| Access Level: | acceso abierto |
| Palabra clave: | Angola Field conditions Loop-mediated isothermal amplification LAMP Malaria Plasmodium spp. Point-of-care Plasmodium Nucleic Acid Amplification Techniques Molecular Diagnostic Techniques 24 Ciencias de la vida 2401.12 Parasitología Animal malaria técnicas de diagnóstico molecular técnicas de amplificación de ácidos nucleicos |
| Sumario: | [EN]Malaria is a globally distributed infectious disease. According to the World Health Organization, Angola is one of the six countries that account for over half the global malaria burden in terms of both malaria cases and deaths. Diagnosis of malaria still depends on microscopic examination of thin and thick blood smears and rapid diagnostic tests (RDTs), which often lack analytical and clinical sensitivity. Molecular methods could overcome these disadvantages. The aim of this study was to evaluate, for the first time to our knowledge, the performance of a loop-mediated isothermal amplification (LAMP) for the diagnosis of malaria in an endemic area in Cubal, Angola, and to assess the reproducibility at a reference laboratory. |
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