ZnO Nanoporous Spheres with Broad-Spectrum Antimicrobial Activity by Physicochemical Interactions

The extensive range of applications where synthetic nanomaterials are nowadays used is causing a huge commercial market. An incipient use of these nanomaterials arises from the need to generate alternative antimicrobial agents, anticipating the development of resistant micro- organisms. Here, we sho...

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Detalles Bibliográficos
Autores: Lucas-Gil, Eva de, Leret, Pilar, Monte-Serrano, Mercedes, Jiménez Reinosa, Julián, Enríquez Pérez, Esther, Campo, Ángel Adolfo del, Cañete, Magdalena, Menéndez, Javier, Fernández Lozano, José Francisco, Rubio Marcos, Fernando
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/191990
Acceso en línea:http://hdl.handle.net/10261/191990
Access Level:acceso abierto
Palabra clave:Physicochemical action
Antimicrobial properties
Nanoporous spheres
ZnO
Cluster
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network_acronym_str ES
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repository_id_str
spelling ZnO Nanoporous Spheres with Broad-Spectrum Antimicrobial Activity by Physicochemical InteractionsLucas-Gil, Eva deLeret, PilarMonte-Serrano, MercedesJiménez Reinosa, JuliánEnríquez Pérez, EstherCampo, Ángel Adolfo delCañete, MagdalenaMenéndez, JavierFernández Lozano, José FranciscoRubio Marcos, FernandoPhysicochemical actionAntimicrobial propertiesNanoporous spheresZnOClusterThe extensive range of applications where synthetic nanomaterials are nowadays used is causing a huge commercial market. An incipient use of these nanomaterials arises from the need to generate alternative antimicrobial agents, anticipating the development of resistant micro- organisms. Here, we show a nanostructured ZnO with antimicrobial properties and low cytotoxicity based on a nanoparticle¿s arrangement by controlling the formation of sintering neck into nanoporous spheres. The antimicrobial effectiveness of ZnO spheres is tested in a broad spectrum of microorganisms such as fungi as well as Gram-negative and Gram-positive bacteria. The hierarchical structures show highly effective antimicrobial activity at low concentrations and in relatively short action times (24¿72 h). We demonstrate that the enhanced antimicrobial properties against microorganisms are ascribed to a combining of both physical and chemical interactions between microorganism and ZnO. The approximation mechanism between microorganism and ZnO is provided through electrostatic forces (physical interaction) which, thanks to the ZnO¿microorganism proximity, promote a rapid release of zinc cations and the reactive oxygen species penetration into microorganisms (chemical interaction). We believe that this work provides insights into the mechanisms underlying the interactions ZnO¿microorganism and possesses a combined action mechanism for which nanostructured ZnO is so effective to combat microorganisms.This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) under the projects MAT2017-86450-C4-1-R, and the Spanish National Research Council (CSIC) under the project NANOMIND CSIC 201560E068. Dr. P. Leret is also indebted to MINECO for a “Torres Quevedo” contract (ref: PTQ-12-05470), which is cofinanced with European Social Fund. Dr. E. Enriquez is also indebted to MINECO for a ‘‘Torres Quevedo’’ contract (ref: PTQ-14-07289), which is co-financed with European Social Fund. Dr. F. R-M is also indebted to MINECO for a ‘Ramon y Cajal’ contract (ref: RyC-2015-18626), which is co-financed by the European Social. The authors acknowledge the ESRF The European Synchrotron and the SpLine CRG BM25 beamline staff for provision of synchrotron radiation and for assistance during X-ray diffraction measurements (ref: 25-01-966). Finally, authors are grateful to Aida Serrano and Rocío Estefanía Rojas-Hernandez for scientific and technical advice in XANES measurements, Mª José Velasco and Pilar Ortega for ICP-OES measurements, Alexandra Muñoz-Bonilla for scientific advice and Laura Pascual for scientific and technical advicePeer ReviewedAmerican Chemical SocietyAgencia Estatal de Investigación (España)Ministerio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas (España)Lucas-Gil, Eva de [0000-0001-5894-9866]Fernández Lozano, José Francisco [0000-0001-5894-9866]Rubio Marcos, Fernando [0000-0002-2479-3792]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920182019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/191990reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#MAT2017-86450-C4-1-R/AEI/10.13039/501100011033info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/MAT2017-86450-C4-1-Rhttps://doi.org/10.1021/acsanm.8b00402Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1919902026-05-22T06:33:51Z
dc.title.none.fl_str_mv ZnO Nanoporous Spheres with Broad-Spectrum Antimicrobial Activity by Physicochemical Interactions
title ZnO Nanoporous Spheres with Broad-Spectrum Antimicrobial Activity by Physicochemical Interactions
spellingShingle ZnO Nanoporous Spheres with Broad-Spectrum Antimicrobial Activity by Physicochemical Interactions
Lucas-Gil, Eva de
Physicochemical action
Antimicrobial properties
Nanoporous spheres
ZnO
Cluster
title_short ZnO Nanoporous Spheres with Broad-Spectrum Antimicrobial Activity by Physicochemical Interactions
title_full ZnO Nanoporous Spheres with Broad-Spectrum Antimicrobial Activity by Physicochemical Interactions
title_fullStr ZnO Nanoporous Spheres with Broad-Spectrum Antimicrobial Activity by Physicochemical Interactions
title_full_unstemmed ZnO Nanoporous Spheres with Broad-Spectrum Antimicrobial Activity by Physicochemical Interactions
title_sort ZnO Nanoporous Spheres with Broad-Spectrum Antimicrobial Activity by Physicochemical Interactions
dc.creator.none.fl_str_mv Lucas-Gil, Eva de
Leret, Pilar
Monte-Serrano, Mercedes
Jiménez Reinosa, Julián
Enríquez Pérez, Esther
Campo, Ángel Adolfo del
Cañete, Magdalena
Menéndez, Javier
Fernández Lozano, José Francisco
Rubio Marcos, Fernando
author Lucas-Gil, Eva de
author_facet Lucas-Gil, Eva de
Leret, Pilar
Monte-Serrano, Mercedes
Jiménez Reinosa, Julián
Enríquez Pérez, Esther
Campo, Ángel Adolfo del
Cañete, Magdalena
Menéndez, Javier
Fernández Lozano, José Francisco
Rubio Marcos, Fernando
author_role author
author2 Leret, Pilar
Monte-Serrano, Mercedes
Jiménez Reinosa, Julián
Enríquez Pérez, Esther
Campo, Ángel Adolfo del
Cañete, Magdalena
Menéndez, Javier
Fernández Lozano, José Francisco
Rubio Marcos, Fernando
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas (España)
Lucas-Gil, Eva de [0000-0001-5894-9866]
Fernández Lozano, José Francisco [0000-0001-5894-9866]
Rubio Marcos, Fernando [0000-0002-2479-3792]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Physicochemical action
Antimicrobial properties
Nanoporous spheres
ZnO
Cluster
topic Physicochemical action
Antimicrobial properties
Nanoporous spheres
ZnO
Cluster
description The extensive range of applications where synthetic nanomaterials are nowadays used is causing a huge commercial market. An incipient use of these nanomaterials arises from the need to generate alternative antimicrobial agents, anticipating the development of resistant micro- organisms. Here, we show a nanostructured ZnO with antimicrobial properties and low cytotoxicity based on a nanoparticle¿s arrangement by controlling the formation of sintering neck into nanoporous spheres. The antimicrobial effectiveness of ZnO spheres is tested in a broad spectrum of microorganisms such as fungi as well as Gram-negative and Gram-positive bacteria. The hierarchical structures show highly effective antimicrobial activity at low concentrations and in relatively short action times (24¿72 h). We demonstrate that the enhanced antimicrobial properties against microorganisms are ascribed to a combining of both physical and chemical interactions between microorganism and ZnO. The approximation mechanism between microorganism and ZnO is provided through electrostatic forces (physical interaction) which, thanks to the ZnO¿microorganism proximity, promote a rapid release of zinc cations and the reactive oxygen species penetration into microorganisms (chemical interaction). We believe that this work provides insights into the mechanisms underlying the interactions ZnO¿microorganism and possesses a combined action mechanism for which nanostructured ZnO is so effective to combat microorganisms.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/191990
url http://hdl.handle.net/10261/191990
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
MAT2017-86450-C4-1-R/AEI/10.13039/501100011033
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/MAT2017-86450-C4-1-R
https://doi.org/10.1021/acsanm.8b00402
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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