Salinity influence on Mytilus galloprovincialis exposed to antineoplastic agents: a transcriptomic, biochemical, and histopathological approach

Nowadays, aquatic species face a variety of environmental risks associated with pharmaceutical consumption. More specifically, the increased number of cancer patients has been accompanied by an increased consumption of antineoplastic drugs, such as ifosfamide (IF) and cyclophosphamide (CP). These dr...

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Autores: Queirós, Vanessa, Leite, Carla, Azeiteiro, Ulisses M, Belloso, Marta Casado, Soares, Amadeu M. V. M., Santos, Juan Luis, Alonso, Esteban, Barata Martí, Carlos, Freitas, Rosa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/371930
Acceso en línea:http://hdl.handle.net/10261/371930
https://api.elsevier.com/content/abstract/scopus_id/85207636994
Access Level:acceso abierto
Palabra clave:Salinity shifts
Anticancer drugs
Biomarkers
Climate change
Gene expression
http://metadata.un.org/sdg/13
http://metadata.un.org/sdg/11
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Make cities and human settlements inclusive, safe, resilient and sustainable
Take urgent action to combat climate change and its impacts
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spelling Salinity influence on Mytilus galloprovincialis exposed to antineoplastic agents: a transcriptomic, biochemical, and histopathological approachQueirós, VanessaLeite, CarlaAzeiteiro, Ulisses MBelloso, Marta CasadoSoares, Amadeu M. V. M.Santos, Juan LuisAlonso, EstebanBarata Martí, CarlosFreitas, RosaSalinity shiftsAnticancer drugsBiomarkersClimate changeGene expressionhttp://metadata.un.org/sdg/13http://metadata.un.org/sdg/11http://metadata.un.org/sdg/3Ensure healthy lives and promote well-being for all at all agesMake cities and human settlements inclusive, safe, resilient and sustainableTake urgent action to combat climate change and its impactsNowadays, aquatic species face a variety of environmental risks associated with pharmaceutical consumption. More specifically, the increased number of cancer patients has been accompanied by an increased consumption of antineoplastic drugs, such as ifosfamide (IF) and cyclophosphamide (CP). These drugs have been found in aquatic ecosystems, raising concerns about their impact, especially on estuarine species, as marine waters are the final recipients of continental effluents. Simultaneously, predicted climatic changes, such as salinity shifts, may threaten organisms. Considering this, the present research aims to investigate the combined effects of IF and CP, and salinity shifts. For this, a transcriptomic, biochemical, and histopathological assessment was made using the bivalve species Mytilus galloprovincialis exposed for 28 days to IF and CP (500 ng/L), individually, at different salinity levels (20, 30, and 40). IF and CP up-regulated metabolism-related gene cyp3a1, with CP also affecting abcc gene, showing minimal salinity impact and highlighting the importance of these metabolic routes in mussels. Salinity shifts affected the transcription of genes related to apoptosis and cell cycle growth, such as p53, as well as the aerobic metabolism, the antioxidant and biotransformation mechanisms. These findings indicate mussels' high metabolic adaptability to osmotic stress. Under CP exposure and low salinity, mussels exhibited increased cellular damage and histopathological effects in digestive gland tubules, revealing detrimental effects towards M. galloprovincialis, and suggesting that a metabolic slowdown and activation of antioxidant mechanisms helped prevent oxidative damage at the control and high salinities. Overall, results reinforce the need for antineoplastics ecotoxicological risk assessment, especially under foreseen climate change scenarios.Vanessa Queirós (https://doi.org/10.54499/PD/BD/150590/2020) and Carla Leite (https://doi.org/10.54499/2020.05296.BD) benefited from a PhD grant given by the National Funds through the Portuguese Science Foundation (Fundação para a Ciência e a Tecnologia, FCT). This study was financed by CESAM (UIDP/50017/2020+UIDB/50017/2020+LA/P/0094/2020) and by the Spanish grant PID2020-113371RB-C21, funded by MCIN/AEI/10.13039/501100011033.Peer reviewedElsevierMinisterio de Ciencia y Tecnología (España)0000-0001-6426-05120000-0001-8579-293X0000-0003-0879-94700000-0001-9704-13160000-0002-1647-92260000-0002-3360-07290000-0003-4900-3897Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/371930https://api.elsevier.com/content/abstract/scopus_id/85207636994reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MCIN/AEI/10.13039Environmental pollution (Barking, Essex : 1987)https://doi.org/10.1016/j.envpol.2024.125135Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3719302026-05-22T06:33:51Z
dc.title.none.fl_str_mv Salinity influence on Mytilus galloprovincialis exposed to antineoplastic agents: a transcriptomic, biochemical, and histopathological approach
title Salinity influence on Mytilus galloprovincialis exposed to antineoplastic agents: a transcriptomic, biochemical, and histopathological approach
spellingShingle Salinity influence on Mytilus galloprovincialis exposed to antineoplastic agents: a transcriptomic, biochemical, and histopathological approach
Queirós, Vanessa
Salinity shifts
Anticancer drugs
Biomarkers
Climate change
Gene expression
http://metadata.un.org/sdg/13
http://metadata.un.org/sdg/11
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Make cities and human settlements inclusive, safe, resilient and sustainable
Take urgent action to combat climate change and its impacts
title_short Salinity influence on Mytilus galloprovincialis exposed to antineoplastic agents: a transcriptomic, biochemical, and histopathological approach
title_full Salinity influence on Mytilus galloprovincialis exposed to antineoplastic agents: a transcriptomic, biochemical, and histopathological approach
title_fullStr Salinity influence on Mytilus galloprovincialis exposed to antineoplastic agents: a transcriptomic, biochemical, and histopathological approach
title_full_unstemmed Salinity influence on Mytilus galloprovincialis exposed to antineoplastic agents: a transcriptomic, biochemical, and histopathological approach
title_sort Salinity influence on Mytilus galloprovincialis exposed to antineoplastic agents: a transcriptomic, biochemical, and histopathological approach
dc.creator.none.fl_str_mv Queirós, Vanessa
Leite, Carla
Azeiteiro, Ulisses M
Belloso, Marta Casado
Soares, Amadeu M. V. M.
Santos, Juan Luis
Alonso, Esteban
Barata Martí, Carlos
Freitas, Rosa
author Queirós, Vanessa
author_facet Queirós, Vanessa
Leite, Carla
Azeiteiro, Ulisses M
Belloso, Marta Casado
Soares, Amadeu M. V. M.
Santos, Juan Luis
Alonso, Esteban
Barata Martí, Carlos
Freitas, Rosa
author_role author
author2 Leite, Carla
Azeiteiro, Ulisses M
Belloso, Marta Casado
Soares, Amadeu M. V. M.
Santos, Juan Luis
Alonso, Esteban
Barata Martí, Carlos
Freitas, Rosa
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia y Tecnología (España)
0000-0001-6426-0512
0000-0001-8579-293X
0000-0003-0879-9470
0000-0001-9704-1316
0000-0002-1647-9226
0000-0002-3360-0729
0000-0003-4900-3897
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Salinity shifts
Anticancer drugs
Biomarkers
Climate change
Gene expression
http://metadata.un.org/sdg/13
http://metadata.un.org/sdg/11
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Make cities and human settlements inclusive, safe, resilient and sustainable
Take urgent action to combat climate change and its impacts
topic Salinity shifts
Anticancer drugs
Biomarkers
Climate change
Gene expression
http://metadata.un.org/sdg/13
http://metadata.un.org/sdg/11
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Make cities and human settlements inclusive, safe, resilient and sustainable
Take urgent action to combat climate change and its impacts
description Nowadays, aquatic species face a variety of environmental risks associated with pharmaceutical consumption. More specifically, the increased number of cancer patients has been accompanied by an increased consumption of antineoplastic drugs, such as ifosfamide (IF) and cyclophosphamide (CP). These drugs have been found in aquatic ecosystems, raising concerns about their impact, especially on estuarine species, as marine waters are the final recipients of continental effluents. Simultaneously, predicted climatic changes, such as salinity shifts, may threaten organisms. Considering this, the present research aims to investigate the combined effects of IF and CP, and salinity shifts. For this, a transcriptomic, biochemical, and histopathological assessment was made using the bivalve species Mytilus galloprovincialis exposed for 28 days to IF and CP (500 ng/L), individually, at different salinity levels (20, 30, and 40). IF and CP up-regulated metabolism-related gene cyp3a1, with CP also affecting abcc gene, showing minimal salinity impact and highlighting the importance of these metabolic routes in mussels. Salinity shifts affected the transcription of genes related to apoptosis and cell cycle growth, such as p53, as well as the aerobic metabolism, the antioxidant and biotransformation mechanisms. These findings indicate mussels' high metabolic adaptability to osmotic stress. Under CP exposure and low salinity, mussels exhibited increased cellular damage and histopathological effects in digestive gland tubules, revealing detrimental effects towards M. galloprovincialis, and suggesting that a metabolic slowdown and activation of antioxidant mechanisms helped prevent oxidative damage at the control and high salinities. Overall, results reinforce the need for antineoplastics ecotoxicological risk assessment, especially under foreseen climate change scenarios.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/371930
https://api.elsevier.com/content/abstract/scopus_id/85207636994
url http://hdl.handle.net/10261/371930
https://api.elsevier.com/content/abstract/scopus_id/85207636994
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MCIN/AEI/10.13039
Environmental pollution (Barking, Essex : 1987)
https://doi.org/10.1016/j.envpol.2024.125135
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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