Effects of water temperature increase on gene expression, biochemical, and histopathological responses of the bivalve species Mytilus galloprovincialis to the antineoplastic drug 5-fluorouracil

The present study aimed to assess the potential ecotoxicological effects of 5-fluorouracil (5-FU), an antineoplastic already found in aquatic ecosystems, towards the bivalve Mytilus galloprovincialis, considering actual and warming temperature scenarios. To this end, organisms were exposed for 28 da...

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Detalles Bibliográficos
Autores: Queirós, Vanessa, Leite, Carla, Azeiteiro, Ulisses M., Belloso, Marta Casado, Navarro-Martín, Laia, Soares, Amadeu M. V. M., Santos, Juan Luis, Alonso, Esteban, Freitas, Rosa, Barata Martí, Carlos
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/394302
Acceso en línea:http://hdl.handle.net/10261/394302
https://api.elsevier.com/content/abstract/scopus_id/105008987214
Access Level:acceso abierto
Palabra clave:Oxidative stress
Anticancer drugs
Climate change
Gene expression
Mollusks
http://metadata.un.org/sdg/13
http://metadata.un.org/sdg/6
http://metadata.un.org/sdg/9
http://metadata.un.org/sdg/3
http://metadata.un.org/sdg/11
Ensure healthy lives and promote well-being for all at all ages
Ensure availability and sustainable management of water and sanitation for all
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
Make cities and human settlements inclusive, safe, resilient and sustainable
Take urgent action to combat climate change and its impacts
Descripción
Sumario:The present study aimed to assess the potential ecotoxicological effects of 5-fluorouracil (5-FU), an antineoplastic already found in aquatic ecosystems, towards the bivalve Mytilus galloprovincialis, considering actual and warming temperature scenarios. To this end, organisms were exposed for 28 days to increasing 5-FU concentrations (10, 100, and 500 ng/L) at control (17 ± 1.0 °C) and warming (21 ± 1.0 °C) temperatures. Transcriptomic of selected target genes, biochemical responses, and histopathological alterations were used to assess possible detrimental effects. Results showed an overall down-regulation of mRNA transcripts related to xenobiotic metabolization pathways and cell apoptosis in organisms exposed to 5-FU, especially at 21 °C. While at 17 °C, the antioxidant defenses were not enough to avoid cell damage caused by 5-FU, at 21 °C the metabolic slowdown avoided further cell damage. However, histopathological alterations in the digestive gland at 21 °C at high concentrations of 5-FU were observed. Overall, the present results indicate that the warming temperature may enhance the detrimental effects of 5-FU on this species, with metabolism being particularly affected.