Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata)

The inclusion of a medicinal plant leaf extract (MPLE) from sage (Salvia officinalis) and lemon verbena (Lippia citriodora), rich in verbascoside and triterpenic compounds like ursolic acid, was evaluated in gilthead seabream (Sparus aurata) fed a low fishmeal-based diet (48% crude protein, 17% crud...

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Autores: Salomón, Ricardo|||0000-0003-1433-2789, Reyes-López, Felipe E.|||0000-0002-5001-457X, Tort Bardolet, Lluís|||0000-0002-3907-722X, Firmino, Joana P.|||0000-0002-7694-0416, Sarasquete, Carmen, Ortiz-Delgado, Juan B., Quintela, José C., Pinilla-Rosas, José M., Vallejos Vidal, Eva Carolina|||0000-0002-3091-2700, Gisbert, Enric|||0000-0002-7457-8468
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:255612
Acceso en línea:https://ddd.uab.cat/record/255612
https://dx.doi.org/urn:doi:10.3389/fimmu.2021.670279
Access Level:acceso abierto
Palabra clave:Cell proliferation
Feed additive
Gut health
Innate immunity
Lectin histochemistry
Ursolic acid
Verbascoside acid
GALT
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spelling Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata)Salomón, Ricardo|||0000-0003-1433-2789Reyes-López, Felipe E.|||0000-0002-5001-457XTort Bardolet, Lluís|||0000-0002-3907-722XFirmino, Joana P.|||0000-0002-7694-0416Sarasquete, CarmenOrtiz-Delgado, Juan B.Quintela, José C.Pinilla-Rosas, José M.Vallejos Vidal, Eva Carolina|||0000-0002-3091-2700Gisbert, Enric|||0000-0002-7457-8468Cell proliferationFeed additiveGut healthInnate immunityLectin histochemistryUrsolic acidVerbascoside acidGALTThe inclusion of a medicinal plant leaf extract (MPLE) from sage (Salvia officinalis) and lemon verbena (Lippia citriodora), rich in verbascoside and triterpenic compounds like ursolic acid, was evaluated in gilthead seabream (Sparus aurata) fed a low fishmeal-based diet (48% crude protein, 17% crude fat, 21.7 MJ kg -1, 7% fishmeal, 15% fish oil) for 92 days. In particular, the study focused on the effect of these phytogenic compounds on the gut condition by analyzing the transcriptomic profiling (microarray analysis) and histological structure of the intestinal mucosa, as well as the histochemical properties of mucins stored in goblet cells. A total number of 506 differentially expressed genes (285 up- and 221 down-regulated) were found when comparing the transcriptomic profiling of the intestine from fish fed the control and MPLE diets. The gut transcripteractome revealed an expression profile that favored biological mechanisms associated to the 1) immune system, particularly involving T cell activation and differentiation, 2) gut integrity (i.e., adherens and tight junctions) and cellular proliferation, and 3) cellular proteolytic pathways. The histological analysis showed that the MPLE dietary supplementation promoted an increase in the number of intestinal goblet cells and modified the composition of mucins' glycoproteins stored in goblet cells, with an increase in the staining intensity of neutral mucins, as well as in mucins rich in carboxylated and weakly sulfated glycoconjugates, particularly those rich in sialic acid residues. The integration of transcriptomic and histological results showed that the evaluated MPLE from sage and lemon verbena is responsible for the maintenance of intestinal health, supporting gut homeostasis and increasing the integrity of the intestinal epithelium, which suggests that this phytogenic may be considered as a promising sustainable functional additive for aquafeeds. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/255612https://dx.doi.org/urn:doi:10.3389/fimmu.2021.670279reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengEuropean Commission https://doi.org/10.13039/501100000780 727315Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/DI-017open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2556122026-06-06T12:50:31Z
dc.title.none.fl_str_mv Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata)
title Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata)
spellingShingle Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata)
Salomón, Ricardo|||0000-0003-1433-2789
Cell proliferation
Feed additive
Gut health
Innate immunity
Lectin histochemistry
Ursolic acid
Verbascoside acid
GALT
title_short Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata)
title_full Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata)
title_fullStr Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata)
title_full_unstemmed Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata)
title_sort Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata)
dc.creator.none.fl_str_mv Salomón, Ricardo|||0000-0003-1433-2789
Reyes-López, Felipe E.|||0000-0002-5001-457X
Tort Bardolet, Lluís|||0000-0002-3907-722X
Firmino, Joana P.|||0000-0002-7694-0416
Sarasquete, Carmen
Ortiz-Delgado, Juan B.
Quintela, José C.
Pinilla-Rosas, José M.
Vallejos Vidal, Eva Carolina|||0000-0002-3091-2700
Gisbert, Enric|||0000-0002-7457-8468
author Salomón, Ricardo|||0000-0003-1433-2789
author_facet Salomón, Ricardo|||0000-0003-1433-2789
Reyes-López, Felipe E.|||0000-0002-5001-457X
Tort Bardolet, Lluís|||0000-0002-3907-722X
Firmino, Joana P.|||0000-0002-7694-0416
Sarasquete, Carmen
Ortiz-Delgado, Juan B.
Quintela, José C.
Pinilla-Rosas, José M.
Vallejos Vidal, Eva Carolina|||0000-0002-3091-2700
Gisbert, Enric|||0000-0002-7457-8468
author_role author
author2 Reyes-López, Felipe E.|||0000-0002-5001-457X
Tort Bardolet, Lluís|||0000-0002-3907-722X
Firmino, Joana P.|||0000-0002-7694-0416
Sarasquete, Carmen
Ortiz-Delgado, Juan B.
Quintela, José C.
Pinilla-Rosas, José M.
Vallejos Vidal, Eva Carolina|||0000-0002-3091-2700
Gisbert, Enric|||0000-0002-7457-8468
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cell proliferation
Feed additive
Gut health
Innate immunity
Lectin histochemistry
Ursolic acid
Verbascoside acid
GALT
topic Cell proliferation
Feed additive
Gut health
Innate immunity
Lectin histochemistry
Ursolic acid
Verbascoside acid
GALT
description The inclusion of a medicinal plant leaf extract (MPLE) from sage (Salvia officinalis) and lemon verbena (Lippia citriodora), rich in verbascoside and triterpenic compounds like ursolic acid, was evaluated in gilthead seabream (Sparus aurata) fed a low fishmeal-based diet (48% crude protein, 17% crude fat, 21.7 MJ kg -1, 7% fishmeal, 15% fish oil) for 92 days. In particular, the study focused on the effect of these phytogenic compounds on the gut condition by analyzing the transcriptomic profiling (microarray analysis) and histological structure of the intestinal mucosa, as well as the histochemical properties of mucins stored in goblet cells. A total number of 506 differentially expressed genes (285 up- and 221 down-regulated) were found when comparing the transcriptomic profiling of the intestine from fish fed the control and MPLE diets. The gut transcripteractome revealed an expression profile that favored biological mechanisms associated to the 1) immune system, particularly involving T cell activation and differentiation, 2) gut integrity (i.e., adherens and tight junctions) and cellular proliferation, and 3) cellular proteolytic pathways. The histological analysis showed that the MPLE dietary supplementation promoted an increase in the number of intestinal goblet cells and modified the composition of mucins' glycoproteins stored in goblet cells, with an increase in the staining intensity of neutral mucins, as well as in mucins rich in carboxylated and weakly sulfated glycoconjugates, particularly those rich in sialic acid residues. The integration of transcriptomic and histological results showed that the evaluated MPLE from sage and lemon verbena is responsible for the maintenance of intestinal health, supporting gut homeostasis and increasing the integrity of the intestinal epithelium, which suggests that this phytogenic may be considered as a promising sustainable functional additive for aquafeeds.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/255612
https://dx.doi.org/urn:doi:10.3389/fimmu.2021.670279
url https://ddd.uab.cat/record/255612
https://dx.doi.org/urn:doi:10.3389/fimmu.2021.670279
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 727315
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/DI-017
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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