Effect of nutrition and Enteromyxum leei infection on gilthead sea bream Sparus aurata intestinal carbohydrate distribution
The effect of a practical plant protein-based diet containing vegetable oils (VO) as the major lipid source on the mucosal carbohydrate pattern of the intestine was studied in gilthead sea bream (Sparus aurata) challenged with the myxosporean parasite Enteromyxum leei. Fish fed for 9 months either a...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2012 |
| País: | España |
| Institución: | Universidad Católica de Valencia San Vicente Mártir |
| Repositorio: | RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir |
| Idioma: | inglés |
| OAI Identifier: | oai:riucv.ucv.es:20.500.12466/4708 |
| Acceso en línea: | http://hdl.handle.net/20.500.12466/4708 |
| Access Level: | acceso abierto |
| Palabra clave: | Goblet cell Replacement diet Mucin Histochemistry Lectin Nutrition 2401.05 desarrollo Animal 3104.06 Nutrición 2510.92 Acuicultura Marina |
| Sumario: | The effect of a practical plant protein-based diet containing vegetable oils (VO) as the major lipid source on the mucosal carbohydrate pattern of the intestine was studied in gilthead sea bream (Sparus aurata) challenged with the myxosporean parasite Enteromyxum leei. Fish fed for 9 months either a fish oil (FO) diet or a blend of VO at 66% of replacement (66VO diet) were exposed to parasite-contaminated water effluent. Samples of the intestine (anterior = AI, medium = MI and posterior = PI) were obtained for parasite diagnosis and histochemistry. Fish were categorized as Control (C, not exposed), and early (E), or late (L) infected. Mucin and lectin histochemistry were applied to detect the different types of mucins and sialic acid in goblet cells (GC), the brush border and enterocytes. The number of GC stained with the PAS, AB, AB-AF, for the detection of neutral, acidic, sulphated and carboxylic mucins and with the lectin SNA were counted in digital images. The 66VO diet produced a significant decrease of GC with neutral and acidic mucins at AI and MI, and also of those with carboxylic mucins and sialic acid at MI. Sulphated mucins and sialic acid were less abundant at the AI than at MI and PI in C-66VO. E. leei infection had a strong effect on the number of GC, as E and L infected fish had a significant decrease of GC+ for all the stains vs C fish at PI. A time and a diet effect were also observed, since the lowest values were mostly registered in E-66VO fish at PI |
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