A lupin protein hydrolysate protects the central nervous system from oxidative stress in WD-fed ApoE

Oxidative stress plays a crucial role in neurodegenerative diseases like Parkinson's and Alzheimer's. Studies indicate the relationship between oxidative stress and the brain damage caused by a high-fat diet. It is previously found that a lupin protein hydrolysate (LPH) has antioxidant eff...

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Autores: Santos Sánchez, Guillermo, Ponce España, Eduardo, Álvarez López, Ana Isabel, Pedroche, Justo, Millán Linares, María del Carmen, Fernández Pachón, María Soledad, Lardone, Patricia Judith, Cruz Chamorro, Iván, Carrillo Vico, Antonio
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2024
País:España
Recursos:Universidad de Sevilla (US)
Repositório:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/158745
Acesso em linha:https://hdl.handle.net/11441/158745
https://doi.org/10.1002/mnfr.202300503
Access Level:Acceso aberto
Palavra-chave:Brain
Glutathione
INOS
Nitric oxide
Peptides
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spelling A lupin protein hydrolysate protects the central nervous system from oxidative stress in WD-fed ApoESantos Sánchez, GuillermoPonce España, EduardoÁlvarez López, Ana IsabelPedroche, JustoMillán Linares, María del CarmenFernández Pachón, María SoledadLardone, Patricia JudithCruz Chamorro, IvánCarrillo Vico, AntonioBrainGlutathioneINOSNitric oxidePeptidesOxidative stress plays a crucial role in neurodegenerative diseases like Parkinson's and Alzheimer's. Studies indicate the relationship between oxidative stress and the brain damage caused by a high-fat diet. It is previously found that a lupin protein hydrolysate (LPH) has antioxidant effects on human leukocytes, as well as on the plasma and liver of Western diet (WD)-fed ApoE-/- mice. Additionally, LPH shows anxiolytic effects in these mice. Given the connection between oxidative stress and anxiety, this study aimed to investigate the antioxidant effects of LPH on the brain of WD-fed ApoE-/- mice. LPH (100 mg kg-1) or a vehicle is administered daily for 12 weeks. Peptide analysis of LPH identified 101 amino acid sequences (36.33%) with antioxidant motifs. Treatment with LPH palliated the decrease in total antioxidant activity caused by WD ingestion and regulated the nitric oxide synthesis pathway in the brain of the animals. Furthermore, LPH increased cerebral glutathione levels and the activity of catalase and glutathione reductase antioxidant enzymes and reduced the 8-hydroxy-2'-deoxyguanosine levels, a DNA damage marker. These findings, for the first time, highlight the antioxidant activity of LPH in the brain. This hydrolysate could potentially be used in future nutraceutical therapies for neurodegenerative diseases.Premio Mensual Publicación Científica Destacada de la US. Facultad de MedicinaBioquímica Médica y Biología Molecular e InmunologíaAndalusian GovernmentConsejeria de Economia, Conocimiento, Empresas y Universidad, Junta de AndaluciaConsejeria de Salud y Familias, Junta de AndaluciaMinisterio de Educacion, Cultura y DeporteUniversidad de Sevilla2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/158745https://doi.org/10.1002/mnfr.202300503reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMolecular Nutrition & Food Research, 68 (5), e2300503.CTS160DOC_00587/2020PC-0111-2016-0111PEMP-0085-2020PI-0136-2019FPU16/02339VI PPIT-UShttps://onlinelibrary.wiley.com/doi/10.1002/mnfr.202300503info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1587452026-06-17T12:51:07Z
dc.title.none.fl_str_mv A lupin protein hydrolysate protects the central nervous system from oxidative stress in WD-fed ApoE
title A lupin protein hydrolysate protects the central nervous system from oxidative stress in WD-fed ApoE
spellingShingle A lupin protein hydrolysate protects the central nervous system from oxidative stress in WD-fed ApoE
Santos Sánchez, Guillermo
Brain
Glutathione
INOS
Nitric oxide
Peptides
title_short A lupin protein hydrolysate protects the central nervous system from oxidative stress in WD-fed ApoE
title_full A lupin protein hydrolysate protects the central nervous system from oxidative stress in WD-fed ApoE
title_fullStr A lupin protein hydrolysate protects the central nervous system from oxidative stress in WD-fed ApoE
title_full_unstemmed A lupin protein hydrolysate protects the central nervous system from oxidative stress in WD-fed ApoE
title_sort A lupin protein hydrolysate protects the central nervous system from oxidative stress in WD-fed ApoE
dc.creator.none.fl_str_mv Santos Sánchez, Guillermo
Ponce España, Eduardo
Álvarez López, Ana Isabel
Pedroche, Justo
Millán Linares, María del Carmen
Fernández Pachón, María Soledad
Lardone, Patricia Judith
Cruz Chamorro, Iván
Carrillo Vico, Antonio
author Santos Sánchez, Guillermo
author_facet Santos Sánchez, Guillermo
Ponce España, Eduardo
Álvarez López, Ana Isabel
Pedroche, Justo
Millán Linares, María del Carmen
Fernández Pachón, María Soledad
Lardone, Patricia Judith
Cruz Chamorro, Iván
Carrillo Vico, Antonio
author_role author
author2 Ponce España, Eduardo
Álvarez López, Ana Isabel
Pedroche, Justo
Millán Linares, María del Carmen
Fernández Pachón, María Soledad
Lardone, Patricia Judith
Cruz Chamorro, Iván
Carrillo Vico, Antonio
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Bioquímica Médica y Biología Molecular e Inmunología
Andalusian Government
Consejeria de Economia, Conocimiento, Empresas y Universidad, Junta de Andalucia
Consejeria de Salud y Familias, Junta de Andalucia
Ministerio de Educacion, Cultura y Deporte
Universidad de Sevilla
dc.subject.none.fl_str_mv Brain
Glutathione
INOS
Nitric oxide
Peptides
topic Brain
Glutathione
INOS
Nitric oxide
Peptides
description Oxidative stress plays a crucial role in neurodegenerative diseases like Parkinson's and Alzheimer's. Studies indicate the relationship between oxidative stress and the brain damage caused by a high-fat diet. It is previously found that a lupin protein hydrolysate (LPH) has antioxidant effects on human leukocytes, as well as on the plasma and liver of Western diet (WD)-fed ApoE-/- mice. Additionally, LPH shows anxiolytic effects in these mice. Given the connection between oxidative stress and anxiety, this study aimed to investigate the antioxidant effects of LPH on the brain of WD-fed ApoE-/- mice. LPH (100 mg kg-1) or a vehicle is administered daily for 12 weeks. Peptide analysis of LPH identified 101 amino acid sequences (36.33%) with antioxidant motifs. Treatment with LPH palliated the decrease in total antioxidant activity caused by WD ingestion and regulated the nitric oxide synthesis pathway in the brain of the animals. Furthermore, LPH increased cerebral glutathione levels and the activity of catalase and glutathione reductase antioxidant enzymes and reduced the 8-hydroxy-2'-deoxyguanosine levels, a DNA damage marker. These findings, for the first time, highlight the antioxidant activity of LPH in the brain. This hydrolysate could potentially be used in future nutraceutical therapies for neurodegenerative diseases.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/158745
https://doi.org/10.1002/mnfr.202300503
url https://hdl.handle.net/11441/158745
https://doi.org/10.1002/mnfr.202300503
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Molecular Nutrition & Food Research, 68 (5), e2300503.
CTS160
DOC_00587/2020
PC-0111-2016-0111
PEMP-0085-2020
PI-0136-2019
FPU16/02339
VI PPIT-US
https://onlinelibrary.wiley.com/doi/10.1002/mnfr.202300503
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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