Transcriptomic and proteomic analysis of Oenococcus oeni adaptation to wine stress conditions

Oenococcus oeni, the main lactic acid bacteria responsible for malolactic fermentation in wine, has to adapt to stressful conditions, such as low pH and high ethanol content. In this study, the changes in the transcriptome and the proteome of O. oeni PSU-1 during the adaptation period before MLF sta...

Descripción completa

Detalles Bibliográficos
Autores: Margalef-Català, Mar, Araque, Isabel, Bordons, Albert, Reguant, Cristina, Bautista-Gallego, J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/149703
Acceso en línea:http://hdl.handle.net/10261/149703
Access Level:acceso abierto
Palabra clave:Oenococcus oeni
Transcriptomic
Proteomics
Stress
Wine
Malolactic fermentation
id ES_9db65becef30875b7c299e682fa9aa4b
oai_identifier_str oai:digital.csic.es:10261/149703
network_acronym_str ES
network_name_str España
repository_id_str
spelling Transcriptomic and proteomic analysis of Oenococcus oeni adaptation to wine stress conditionsMargalef-Català, MarAraque, IsabelBordons, AlbertReguant, CristinaBautista-Gallego, J.Oenococcus oeniTranscriptomicProteomicsStressWineMalolactic fermentationOenococcus oeni, the main lactic acid bacteria responsible for malolactic fermentation in wine, has to adapt to stressful conditions, such as low pH and high ethanol content. In this study, the changes in the transcriptome and the proteome of O. oeni PSU-1 during the adaptation period before MLF start have been studied. DNA microarrays were used for the transcriptomic analysis and two complementary proteomic techniques, 2-D DIGE and iTRAQ labeling were used to analyze the proteomic response. One of the most influenced functions in PSU-1 due to inoculation into wine-like medium (WLM) was translation, showing the over-expression of certain ribosomal genes and the corresponding proteins. Amino acid metabolism and transport was also altered and several peptidases were up regulated both at gene and protein level. Certain proteins involved in glutamine and glutamate metabolism showed an increased abundance revealing the key role of nitrogen uptake under stressful conditions. A strong transcriptional inhibition of carbohydrate metabolism related genes was observed. On the other hand, the transcriptional up-regulation of malate transport and citrate consumption was indicative of the use of L-malate and citrate associated to stress response and as an alternative energy source to sugar metabolism. Regarding the stress mechanisms, our results support the relevance of the thioredoxin and glutathione systems in the adaptation of O. oeni to wine related stress. Genes and proteins related to cell wall showed also significant changes indicating the relevance of the cell envelop as protective barrier to environmental stress. The differences found between transcriptomic and proteomic data suggested the relevance of post-transcriptional mechanisms and the complexity of the stress response in O. oeni adaptation. Further research should deepen into the metabolisms mostly altered due to wine conditions to elucidate the role of each mechanism in the O. oeni ability to develop MLF.This work was supported by grants AGL2009-07369ALI and AGL2012-34866ALI from the Spanish Ministry of Economy and Competitiveness. MM is grateful to the pre-doctoral fellowship from the Universitat Rovira i Virgili. JB wish to thank to the Spanish Government for his “Ayuda para la Formación Posdoctoral” research contract.Peer ReviewedFrontiers MediaMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720162017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/149703reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1497032026-05-22T06:33:51Z
dc.title.none.fl_str_mv Transcriptomic and proteomic analysis of Oenococcus oeni adaptation to wine stress conditions
title Transcriptomic and proteomic analysis of Oenococcus oeni adaptation to wine stress conditions
spellingShingle Transcriptomic and proteomic analysis of Oenococcus oeni adaptation to wine stress conditions
Margalef-Català, Mar
Oenococcus oeni
Transcriptomic
Proteomics
Stress
Wine
Malolactic fermentation
title_short Transcriptomic and proteomic analysis of Oenococcus oeni adaptation to wine stress conditions
title_full Transcriptomic and proteomic analysis of Oenococcus oeni adaptation to wine stress conditions
title_fullStr Transcriptomic and proteomic analysis of Oenococcus oeni adaptation to wine stress conditions
title_full_unstemmed Transcriptomic and proteomic analysis of Oenococcus oeni adaptation to wine stress conditions
title_sort Transcriptomic and proteomic analysis of Oenococcus oeni adaptation to wine stress conditions
dc.creator.none.fl_str_mv Margalef-Català, Mar
Araque, Isabel
Bordons, Albert
Reguant, Cristina
Bautista-Gallego, J.
author Margalef-Català, Mar
author_facet Margalef-Català, Mar
Araque, Isabel
Bordons, Albert
Reguant, Cristina
Bautista-Gallego, J.
author_role author
author2 Araque, Isabel
Bordons, Albert
Reguant, Cristina
Bautista-Gallego, J.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Oenococcus oeni
Transcriptomic
Proteomics
Stress
Wine
Malolactic fermentation
topic Oenococcus oeni
Transcriptomic
Proteomics
Stress
Wine
Malolactic fermentation
description Oenococcus oeni, the main lactic acid bacteria responsible for malolactic fermentation in wine, has to adapt to stressful conditions, such as low pH and high ethanol content. In this study, the changes in the transcriptome and the proteome of O. oeni PSU-1 during the adaptation period before MLF start have been studied. DNA microarrays were used for the transcriptomic analysis and two complementary proteomic techniques, 2-D DIGE and iTRAQ labeling were used to analyze the proteomic response. One of the most influenced functions in PSU-1 due to inoculation into wine-like medium (WLM) was translation, showing the over-expression of certain ribosomal genes and the corresponding proteins. Amino acid metabolism and transport was also altered and several peptidases were up regulated both at gene and protein level. Certain proteins involved in glutamine and glutamate metabolism showed an increased abundance revealing the key role of nitrogen uptake under stressful conditions. A strong transcriptional inhibition of carbohydrate metabolism related genes was observed. On the other hand, the transcriptional up-regulation of malate transport and citrate consumption was indicative of the use of L-malate and citrate associated to stress response and as an alternative energy source to sugar metabolism. Regarding the stress mechanisms, our results support the relevance of the thioredoxin and glutathione systems in the adaptation of O. oeni to wine related stress. Genes and proteins related to cell wall showed also significant changes indicating the relevance of the cell envelop as protective barrier to environmental stress. The differences found between transcriptomic and proteomic data suggested the relevance of post-transcriptional mechanisms and the complexity of the stress response in O. oeni adaptation. Further research should deepen into the metabolisms mostly altered due to wine conditions to elucidate the role of each mechanism in the O. oeni ability to develop MLF.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/149703
url http://hdl.handle.net/10261/149703
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869414765897449472
score 15,81155

Similares en LA Referencia