Ultrastructural localisation of carbohydrates in four Myxosporean parasites

[EN] The existence and localisation of carbohydrates in four myxosporean parasites was investigated at transmission electron microscope, using lectin histochemistry techniques. The Myxosporea studied showed different lectin binding patterns. N-acetyl-glucosamine or its polymers were predominant in t...

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Autores: Muñoz Ruíz, María Pilar, Sitjà-Bobadilla, Ariadna, Álvarez-Pellitero, Mª del Pilar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/254040
Acceso en línea:http://hdl.handle.net/10261/254040
Access Level:acceso abierto
Palabra clave:Histochemistry
Lectins
Myxozoa
Teleostei
Ultrastructure
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spelling Ultrastructural localisation of carbohydrates in four Myxosporean parasitesMuñoz Ruíz, María PilarSitjà-Bobadilla, AriadnaÁlvarez-Pellitero, Mª del PilarHistochemistryLectinsMyxozoaTeleosteiUltrastructure[EN] The existence and localisation of carbohydrates in four myxosporean parasites was investigated at transmission electron microscope, using lectin histochemistry techniques. The Myxosporea studied showed different lectin binding patterns. N-acetyl-glucosamine or its polymers were predominant in the valves of Leptotheca sp. and were also present in Sphaerospora dicentrarchi and Polysporoplasma spans ones. Mannose and/or glucose terminals were mainly detected in S. dicentrarchi and P. sparis valves. Polar capsule walls were intensively recognised by Con-A in S. dicentrarchi and with medium intensity in P. sparis and Leptotheca sp. The polar filament was scarcely labelled except in Leptotheca sp. with BS-I. The sporoplasms of the studied parasites were stained with all the lectins tested with each myxosporea, except those of Leptotheca sp. with BS-I. Some structures of the developmental stages of Zschokkella mugilis and Leptotheca sp. were stained with BS-I. The possible role of these carbohydrate terminals found in the studied Myxosporea is discussed.[FR] Une étude ultrastructurale précise la présence et la localisation de fractions glucidiques chez quatre myxosporidies par histochimie avec lectines. Les myxosporidies étudiées montrent différents patrons de reconnaissance avec les lectines. On observe une prédominance de N-acétylglucosamine ou de ses polymères dans les valves de Leptotheca sp. et sa simple présence dans les valves de Sphaerospora dicentrarchi et Polysporoplasma sparis. Les terminaisons mannose et/ou glucose sont surtout trouvées dans les valves de S. dicentrarchi et P. sparis. Les parois des capsules polaires sont intensivement reconnues par la Con-A dans S. dicentrarchi et avec une moindre intensité dans P. sparis et Leptotheca sp. Les filaments polaires montrent un léger signal, exception faite de ceux de Leptotheca sp. intensivement marqués avec BS-I. Les sporoplasmes des parasites étudiés ont été marqués par toutes les lectines trouvées dans chaque parasite, excepté ceux de Leptotheca sp. avec BS-I. Cette lectine marque quelques structures des stades de développement de Z. schokkella mugilis et Leptotheca sp. On discute le rôle des fractions glucidiques trouvées dans les myxosporidies étudiées, et on suggère la nécessité de nouvelles études pour éclaircir leur implication dans les relations hôte-parasite.This work was supported by research grants from the Spanish Ministerio de Educación y Cultura No. AGF95-0058 and MAR98/1000. Pilar Munoz received a grant from the Spanish Ministerio de Educación y Cultura. We are thankful to technicians from the Electron Microscopy Service of the University of Barcelona and to the Instituto Español Oceanográfico at Mazarrón (Murcia) for providing some of the fishEDP SciencesMinisterio de Educación y Cultura (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120002021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/254040reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1051/parasite/2000073185Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2540402026-05-22T06:33:51Z
dc.title.none.fl_str_mv Ultrastructural localisation of carbohydrates in four Myxosporean parasites
title Ultrastructural localisation of carbohydrates in four Myxosporean parasites
spellingShingle Ultrastructural localisation of carbohydrates in four Myxosporean parasites
Muñoz Ruíz, María Pilar
Histochemistry
Lectins
Myxozoa
Teleostei
Ultrastructure
title_short Ultrastructural localisation of carbohydrates in four Myxosporean parasites
title_full Ultrastructural localisation of carbohydrates in four Myxosporean parasites
title_fullStr Ultrastructural localisation of carbohydrates in four Myxosporean parasites
title_full_unstemmed Ultrastructural localisation of carbohydrates in four Myxosporean parasites
title_sort Ultrastructural localisation of carbohydrates in four Myxosporean parasites
dc.creator.none.fl_str_mv Muñoz Ruíz, María Pilar
Sitjà-Bobadilla, Ariadna
Álvarez-Pellitero, Mª del Pilar
author Muñoz Ruíz, María Pilar
author_facet Muñoz Ruíz, María Pilar
Sitjà-Bobadilla, Ariadna
Álvarez-Pellitero, Mª del Pilar
author_role author
author2 Sitjà-Bobadilla, Ariadna
Álvarez-Pellitero, Mª del Pilar
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Educación y Cultura (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Histochemistry
Lectins
Myxozoa
Teleostei
Ultrastructure
topic Histochemistry
Lectins
Myxozoa
Teleostei
Ultrastructure
description [EN] The existence and localisation of carbohydrates in four myxosporean parasites was investigated at transmission electron microscope, using lectin histochemistry techniques. The Myxosporea studied showed different lectin binding patterns. N-acetyl-glucosamine or its polymers were predominant in the valves of Leptotheca sp. and were also present in Sphaerospora dicentrarchi and Polysporoplasma spans ones. Mannose and/or glucose terminals were mainly detected in S. dicentrarchi and P. sparis valves. Polar capsule walls were intensively recognised by Con-A in S. dicentrarchi and with medium intensity in P. sparis and Leptotheca sp. The polar filament was scarcely labelled except in Leptotheca sp. with BS-I. The sporoplasms of the studied parasites were stained with all the lectins tested with each myxosporea, except those of Leptotheca sp. with BS-I. Some structures of the developmental stages of Zschokkella mugilis and Leptotheca sp. were stained with BS-I. The possible role of these carbohydrate terminals found in the studied Myxosporea is discussed.
publishDate 2000
dc.date.none.fl_str_mv 2000
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/254040
url http://hdl.handle.net/10261/254040
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1051/parasite/2000073185
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv EDP Sciences
publisher.none.fl_str_mv EDP Sciences
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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