Ultrastructural localisation of carbohydrates in four Myxosporean parasites
[EN] The existence and localisation of carbohydrates in four myxosporean parasites was investigated at transmission electron microscope, using lectin histochemistry techniques. The Myxosporea studied showed different lectin binding patterns. N-acetyl-glucosamine or its polymers were predominant in t...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2000 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/254040 |
| Acceso en línea: | http://hdl.handle.net/10261/254040 |
| Access Level: | acceso abierto |
| Palabra clave: | Histochemistry Lectins Myxozoa Teleostei Ultrastructure |
| Sumario: | [EN] The existence and localisation of carbohydrates in four myxosporean parasites was investigated at transmission electron microscope, using lectin histochemistry techniques. The Myxosporea studied showed different lectin binding patterns. N-acetyl-glucosamine or its polymers were predominant in the valves of Leptotheca sp. and were also present in Sphaerospora dicentrarchi and Polysporoplasma spans ones. Mannose and/or glucose terminals were mainly detected in S. dicentrarchi and P. sparis valves. Polar capsule walls were intensively recognised by Con-A in S. dicentrarchi and with medium intensity in P. sparis and Leptotheca sp. The polar filament was scarcely labelled except in Leptotheca sp. with BS-I. The sporoplasms of the studied parasites were stained with all the lectins tested with each myxosporea, except those of Leptotheca sp. with BS-I. Some structures of the developmental stages of Zschokkella mugilis and Leptotheca sp. were stained with BS-I. The possible role of these carbohydrate terminals found in the studied Myxosporea is discussed. |
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