Expression of serotonin 5-HT2C receptors in GABAergic cells of the anterior raphe nuclei

We have used double in situ hybridization to examine the cellular localization of 5-HT2C receptor mRNA in relation to serotonergic and GABAergic neurons in the anterior raphe nuclei of the rat. In the dorsal and median raphe nuclei 5-HT2C receptor mRNA was not detected in serotonergic cells identifi...

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Detalles Bibliográficos
Autores: Serrats, Jordi, Mengod Los Arcos, Guadalupe, Cortés, Roser
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2005
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/112670
Acceso en línea:http://hdl.handle.net/10261/112670
Access Level:acceso abierto
Palabra clave:GAD
5-HT transporter
5,7-DHT
In situ hybridization
Serotonergic lesions
Median raphe
Dorsal raphe
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spelling Expression of serotonin 5-HT2C receptors in GABAergic cells of the anterior raphe nucleiSerrats, JordiMengod Los Arcos, GuadalupeCortés, RoserGAD5-HT transporter5,7-DHTIn situ hybridizationSerotonergic lesionsMedian rapheDorsal rapheWe have used double in situ hybridization to examine the cellular localization of 5-HT2C receptor mRNA in relation to serotonergic and GABAergic neurons in the anterior raphe nuclei of the rat. In the dorsal and median raphe nuclei 5-HT2C receptor mRNA was not detected in serotonergic cells identified as those expressing serotonin (5-HT) transporter mRNA. In contrast, 5-HT2C receptor mRNA was found in most GABAergic cells, recognized by the presence of glutamic acid decarboxylase mRNA. Such 5-HT2C receptor-positive GABAergic neurons were mainly located in the intermediolateral and lateral portions of the dorsal raphe and lateral part of the median raphe. The present data give anatomical support to a previous hypothesis that proposed a negative-feedback loop involving reciprocal connections between GABAergic interneurons bearing 5-HT2A/2C receptors and 5-HT neurons in the dorsal raphe and surrounding areas. According to this model, the excitation of GABAergic interneurons through these 5-HT 2C (and also 5-HT2A) receptors would result in the suppression of 5-HT cell firing. © 2004 Elsevier B.V. All rights reserved.This work was supported by grants SAF97-0117 (CICYT) and SAF2000-0212 (‘‘Ministerio de Ciencia y Tecnología’’ (MCYT)). Support from DURSI ‘‘Generalitat de Catalunya’’ to the Department of Neurochemistry of IIBB–CSIC (IDIBAPS) as a ‘‘Grup de Recerca Consolidat’’ (5092-CSIC-01) is also acknowledged (Grant 2001-SGR-00355). Jordi Serrats has been recipient of a fellowship ‘‘Beca de Formació de Personal Investigador’’ from ‘‘Institut d’Investigacions Biomèdiques August Pi i Sunyer’’ (IDIBAPS)Peer ReviewedElsevier2015201520052015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/112670reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.jchemneu.2004.03.010info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1126702026-05-22T06:33:51Z
dc.title.none.fl_str_mv Expression of serotonin 5-HT2C receptors in GABAergic cells of the anterior raphe nuclei
title Expression of serotonin 5-HT2C receptors in GABAergic cells of the anterior raphe nuclei
spellingShingle Expression of serotonin 5-HT2C receptors in GABAergic cells of the anterior raphe nuclei
Serrats, Jordi
GAD
5-HT transporter
5,7-DHT
In situ hybridization
Serotonergic lesions
Median raphe
Dorsal raphe
title_short Expression of serotonin 5-HT2C receptors in GABAergic cells of the anterior raphe nuclei
title_full Expression of serotonin 5-HT2C receptors in GABAergic cells of the anterior raphe nuclei
title_fullStr Expression of serotonin 5-HT2C receptors in GABAergic cells of the anterior raphe nuclei
title_full_unstemmed Expression of serotonin 5-HT2C receptors in GABAergic cells of the anterior raphe nuclei
title_sort Expression of serotonin 5-HT2C receptors in GABAergic cells of the anterior raphe nuclei
dc.creator.none.fl_str_mv Serrats, Jordi
Mengod Los Arcos, Guadalupe
Cortés, Roser
author Serrats, Jordi
author_facet Serrats, Jordi
Mengod Los Arcos, Guadalupe
Cortés, Roser
author_role author
author2 Mengod Los Arcos, Guadalupe
Cortés, Roser
author2_role author
author
dc.subject.none.fl_str_mv GAD
5-HT transporter
5,7-DHT
In situ hybridization
Serotonergic lesions
Median raphe
Dorsal raphe
topic GAD
5-HT transporter
5,7-DHT
In situ hybridization
Serotonergic lesions
Median raphe
Dorsal raphe
description We have used double in situ hybridization to examine the cellular localization of 5-HT2C receptor mRNA in relation to serotonergic and GABAergic neurons in the anterior raphe nuclei of the rat. In the dorsal and median raphe nuclei 5-HT2C receptor mRNA was not detected in serotonergic cells identified as those expressing serotonin (5-HT) transporter mRNA. In contrast, 5-HT2C receptor mRNA was found in most GABAergic cells, recognized by the presence of glutamic acid decarboxylase mRNA. Such 5-HT2C receptor-positive GABAergic neurons were mainly located in the intermediolateral and lateral portions of the dorsal raphe and lateral part of the median raphe. The present data give anatomical support to a previous hypothesis that proposed a negative-feedback loop involving reciprocal connections between GABAergic interneurons bearing 5-HT2A/2C receptors and 5-HT neurons in the dorsal raphe and surrounding areas. According to this model, the excitation of GABAergic interneurons through these 5-HT 2C (and also 5-HT2A) receptors would result in the suppression of 5-HT cell firing. © 2004 Elsevier B.V. All rights reserved.
publishDate 2005
dc.date.none.fl_str_mv 2005
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/112670
url http://hdl.handle.net/10261/112670
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.jchemneu.2004.03.010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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