Comprehensive and systematic characterization of multi-functionalized cisplatin nano-conjugate: from the chemistry and proteomic biocompatibility to the animal model

[EN]Background: Nowadays, nanoparticles (NPs) have evolved as multifunctional systems combining diferent custom anchorages which opens a wide range of applications in biomedical research. Thus, their pharmacological involve‑ ments require more comprehensive analysis and novel nanodrugs should be cha...

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Detalles Bibliográficos
Autores: Hernández García, Ángela Patricia, Micaelo, Ania, Piñol, Rafael, García-Vaquero, Marina L., Aramayona, José J., Criado Talavera, Julio José, Rodriguez, Emilio, Sánchez Gallego, José Ignacio, Landeira Viñuela, Alicia, Juanes-Velasco, Pablo, Díez García, Paula, Góngora Fernández, Rafael, Jara-Acevedo, Ricardo, Orfao de Matos Correia e Vale, José Alberto, Miana-Mena, Javier, Muñoz, María Jesús, Villanueva, Sergio, Millán, Ángel, Fuentes García, Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/161927
Acceso en línea:http://hdl.handle.net/10366/161927
Access Level:acceso abierto
Palabra clave:Inmunocompatibilidad
Muerte celular inmunogenica
Nanotecnologia
Proteomica
2412.10 Vacunas
Descripción
Sumario:[EN]Background: Nowadays, nanoparticles (NPs) have evolved as multifunctional systems combining diferent custom anchorages which opens a wide range of applications in biomedical research. Thus, their pharmacological involve‑ ments require more comprehensive analysis and novel nanodrugs should be characterized by both chemically and biological point of view. Within the wide variety of biocompatible nanosystems, iron oxide nanoparticles (IONPs) present mostly of the required features which make them suitable for multifunctional NPs with many biopharmaceu‑ tical applications. Results: CisplatinIONPs and diferent functionalization stages have been broadly evaluated. The potential applica‑ tion of these nanodrugs in oncotherapies has been assessed by studying in vitro biocompatibility (interactions with environment) by proteomics characterization the determination of protein corona in diferent proximal fuids (human plasma, rabbit plasma and fetal bovine serum),. Moreover, protein labeling and LC–MS/MS analysis provided more than 4000 proteins de novo synthetized as consequence of the nanodrugs presence defending cell signaling in dif‑ ferent tumor cell types (data available via ProteomeXchanges with identifed PXD026615). Further in vivo studies have provided a more integrative view of the biopharmaceutical perspectives of IONPs. Conclusions: Pharmacological proteomic profle diferent behavior between species and diferent afnity of protein coating layers (soft and hard corona). Also, intracellular signaling exposed diferences between tumor cell lines.

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