Ocoxin oral solution triggers DNA damage and cell death in ovarian cancer
[EN]Ovarian cancer is the most fatal of all the reproductive cancers within the female population, mainly due to its late diagnosis that limits surgery and medical treatment. Classically, ovarian cancer therapy has included conventional chemotherapy, and other therapeutic approaches are now being us...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad de Salamanca (USAL) |
| Repositorio: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:dnet:gredos______::e419002093c5c620bf64122b82b0289b |
| Acceso en línea: | http://hdl.handle.net/10366/171522 |
| Access Level: | acceso abierto |
| Palabra clave: | DNA damage OOS Antioxidants Cell death Ovarian cancer Zinc Sulfate Ascorbic Acid Xenograft Model Antitumor Assays Histones Plant Extracts Humans DNA Damage Vitamin B 6 Cell Line Antineoplastic Agents Cell Proliferation Folic Acid Cell Death Vitamin B 12 Pantothenic Acid Pyridoxine Ovarian Neoplasms Animals Mice 3201.01 Oncología 24 Ciencias de la Vida ácido pantoténico daño del ADN humanos piridoxina ratones línea celular vitamina B 6 extractos de plantas ácido fólico ensayos antitumorales por modelo de xenoinjerto neoplasias ováricas histonas animales ácido ascórbico antineoplásicos vitamina B 12 sulfato de zinc muerte celular proliferación celular |
| Sumario: | [EN]Ovarian cancer is the most fatal of all the reproductive cancers within the female population, mainly due to its late diagnosis that limits surgery and medical treatment. Classically, ovarian cancer therapy has included conventional chemotherapy, and other therapeutic approaches are now being used to treat these patients, but the outcomes of the disease are still poor. Therefore, new strategies are needed to improve life expectancy and life quality of ovarian cancer patients. Considering that, we investigated the effect of the nutritional supplement Ocoxin Oral Solution (OOS) in ovarian cancer models. OOS contains several nutritional supplements, some of them with demonstrated antitumoral action. In vitro studies showed that OOS inhibited the proliferation of several ovarian cancer cell lines, especially of those representative of the endometrioid subtype, in a time- and dose-dependent manner. A fast cell death induction after OOS treatment was observed, and when the molecular mechanisms leading to this effect were investigated, an activation of the DNA damage checkpoint was detected, as shown by activation (phosphorylation) of CHK1 and CHK2 kinases that was followed by the phosphorylation of the target protein histone H2AX. When tested in animal models of ovarian cancer, OOS reduced tumor growth without any observed secondary effects. Moreover, such reduction in tumor proliferation was caused by the induction of DNA damage as corroborated by the in vivo phosphorylation of CHK2 and Histone H2AX. Finally, OOS potentiated the action of carboplatin or olaparib, the standard of care treatments used in ovarian clinics, opening the possibility of including OOS in combination with those standard of care agents in patients with ovarian cancer. |
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