Production and digestibility studies of β-galactosyl xylitol derivatives using heterogeneous catalysts of LacA β-galactosidase from Lactobacillus plantarum WCFS1

This article belongs to the Special Issue Exclusive Papers of the Editorial Board Members (EBMs) of the Bioorganic Chemistry Section of Molecules (2022).

Detalles Bibliográficos
Autores: Rosado, Eduardo, Delgado, Paloma, De Las Rivas, Blanca, Muñoz, Rosario, Moreno, F. Javier, Corzo, Nieves, Mateo González, César
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/279259
Acceso en línea:http://hdl.handle.net/10261/279259
Access Level:acceso abierto
Palabra clave:Galactosyl xylitol
Digestibility
Galactooligosaccharides
Enzyme immobilization
β-galactosidase
Lactobacillus plantarum
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spelling Production and digestibility studies of β-galactosyl xylitol derivatives using heterogeneous catalysts of LacA β-galactosidase from Lactobacillus plantarum WCFS1Rosado, EduardoDelgado, PalomaDe Las Rivas, BlancaMuñoz, RosarioMoreno, F. JavierCorzo, NievesMateo González, CésarGalactosyl xylitolDigestibilityGalactooligosaccharidesEnzyme immobilizationβ-galactosidaseLactobacillus plantarumThis article belongs to the Special Issue Exclusive Papers of the Editorial Board Members (EBMs) of the Bioorganic Chemistry Section of Molecules (2022).The synthesis of β-galactosyl xylitol derivatives using immobilized LacA β-galactosidase from Lactobacillus plantarum WCFS1 is presented. These compounds have the potential to replace traditional sugars by their properties as sweetener and taking the advantages of a low digestibility. The enzyme was immobilized on different supports, obtaining immobilized preparations with different activity and stability. The immobilization on agarose-IDA-Zn-CHO in the presence of galactose allowed for the conserving of 78% of the offered activity. This preparation was 3.8 times more stable than soluble. Since the enzyme has polyhistidine tags, this support allowed the immobilization, purification and stabilization in one step. The immobilized preparation was used in synthesis obtaining two main products and a total of around 68 g/L of β-galactosyl xylitol derivatives and improving the synthesis/hydrolysis ratio by around 30% compared to that of the soluble enzyme. The catalyst was recycled 10 times, preserving an activity higher than 50%. The in vitro intestinal digestibility of the main β-galactosyl xylitol derivatives was lower than that of lactose, being around 6 and 15% for the galacto-xylitol derivatives compared to 55% of lactose after 120 min of digestion. The optimal amount immobilized constitutes a very useful tool to synthetize β-galactosyl xylitol derivatives since it can be used as a catalyst with high yield and being recycled for at least 10 more cycles.This research was funded by the Spanish Ministry of Science and Innovation (Projects AGL-2017-84614-C2-1-R and AGL-2017-84614-C2-2-R).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/279259reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84614-C2-1-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84614-C2-2-Rhttps://doi.org/10.3390/molecules27041235Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2792592026-05-22T06:33:51Z
dc.title.none.fl_str_mv Production and digestibility studies of β-galactosyl xylitol derivatives using heterogeneous catalysts of LacA β-galactosidase from Lactobacillus plantarum WCFS1
title Production and digestibility studies of β-galactosyl xylitol derivatives using heterogeneous catalysts of LacA β-galactosidase from Lactobacillus plantarum WCFS1
spellingShingle Production and digestibility studies of β-galactosyl xylitol derivatives using heterogeneous catalysts of LacA β-galactosidase from Lactobacillus plantarum WCFS1
Rosado, Eduardo
Galactosyl xylitol
Digestibility
Galactooligosaccharides
Enzyme immobilization
β-galactosidase
Lactobacillus plantarum
title_short Production and digestibility studies of β-galactosyl xylitol derivatives using heterogeneous catalysts of LacA β-galactosidase from Lactobacillus plantarum WCFS1
title_full Production and digestibility studies of β-galactosyl xylitol derivatives using heterogeneous catalysts of LacA β-galactosidase from Lactobacillus plantarum WCFS1
title_fullStr Production and digestibility studies of β-galactosyl xylitol derivatives using heterogeneous catalysts of LacA β-galactosidase from Lactobacillus plantarum WCFS1
title_full_unstemmed Production and digestibility studies of β-galactosyl xylitol derivatives using heterogeneous catalysts of LacA β-galactosidase from Lactobacillus plantarum WCFS1
title_sort Production and digestibility studies of β-galactosyl xylitol derivatives using heterogeneous catalysts of LacA β-galactosidase from Lactobacillus plantarum WCFS1
dc.creator.none.fl_str_mv Rosado, Eduardo
Delgado, Paloma
De Las Rivas, Blanca
Muñoz, Rosario
Moreno, F. Javier
Corzo, Nieves
Mateo González, César
author Rosado, Eduardo
author_facet Rosado, Eduardo
Delgado, Paloma
De Las Rivas, Blanca
Muñoz, Rosario
Moreno, F. Javier
Corzo, Nieves
Mateo González, César
author_role author
author2 Delgado, Paloma
De Las Rivas, Blanca
Muñoz, Rosario
Moreno, F. Javier
Corzo, Nieves
Mateo González, César
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Galactosyl xylitol
Digestibility
Galactooligosaccharides
Enzyme immobilization
β-galactosidase
Lactobacillus plantarum
topic Galactosyl xylitol
Digestibility
Galactooligosaccharides
Enzyme immobilization
β-galactosidase
Lactobacillus plantarum
description This article belongs to the Special Issue Exclusive Papers of the Editorial Board Members (EBMs) of the Bioorganic Chemistry Section of Molecules (2022).
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/279259
url http://hdl.handle.net/10261/279259
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84614-C2-1-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84614-C2-2-R
https://doi.org/10.3390/molecules27041235
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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