Multicenter Clinical Evaluation of a Novel Multiplex Real-Time PCR (qPCR) Assay for Detection of Fluoroquinolone Resistance in
causes a common sexually transmitted infection with a marked propensity to develop antimicrobial resistance. As few treatment options exist, this poses significant challenges to clinicians. Recent diagnostic advances have resulted in tests that report the simultaneous detection of and any resistance...
| Autores: | , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:226588 |
| Acceso en línea: | https://ddd.uab.cat/record/226588 https://dx.doi.org/urn:doi:10.1128/JCM.00886-19 |
| Access Level: | acceso abierto |
| Palabra clave: | Mycoplasma genitalium Fluoroquinolone resistance Multiplex qPCR assay Multicenter evaluation Antibiotic resistance |
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Multicenter Clinical Evaluation of a Novel Multiplex Real-Time PCR (qPCR) Assay for Detection of Fluoroquinolone Resistance inFernández-Huerta, Miguel|||0000-0002-1733-0925Bodiyabadu, KaveeshaEsperalba, Juliana|||0000-0003-1326-1341Bradshaw, Catriona S.Serra-Pladevall, Judit|||0000-0002-4798-2385Garland, Suzanne M.Fernandez-Naval, Candela|||0000-0002-5584-3537Jensen, Jorgen S.|||0000-0002-7464-7435Pumarola Suñé, Tomàs|||0000-0002-5171-7461Ebeyan, SamanthaLundgren, MarieTan, Lit YeenEspasa, Mateu|||0000-0003-4822-1024Murray, Gerald L.Mycoplasma genitaliumFluoroquinolone resistanceMultiplex qPCR assayMulticenter evaluationAntibiotic resistancecauses a common sexually transmitted infection with a marked propensity to develop antimicrobial resistance. As few treatment options exist, this poses significant challenges to clinicians. Recent diagnostic advances have resulted in tests that report the simultaneous detection of and any resistance to macrolides, the first-line treatment. This allows for therapy to be tailored to the individual, thereby optimizing treatment outcomes. causes a common sexually transmitted infection with a marked propensity to develop antimicrobial resistance. As few treatment options exist, this poses significant challenges to clinicians. Recent diagnostic advances have resulted in tests that report the simultaneous detection of and any resistance to macrolides, the first-line treatment. This allows for therapy to be tailored to the individual, thereby optimizing treatment outcomes. However, resistance to fluoroquinolones, the second-line treatment, is increasing in . In this study, we describe a new assay, MG+parC (beta), which simultaneously reports the detection of and five parC mutations that have been associated with resistance to fluoroquinolones. These mutations affect the amino acid sequence of ParC at residues S83R (A247C), S83I (G248T), D87N (G259A), D87Y (G259T), and D87H (G259C). The study tested the MG+parC (beta) assay with 202 -positive clinical samples from Australia (n = 141) and Spain (n = 61). Compared to Sanger sequencing, the assay performed with a kappa value of 0.985 (95% confidence interval [CI], 0.955 to 1.000), with a mutation detection sensitivity of 97.6% (95% CI, 87.4 to 99.9), and specificity of 100.0% (95% CI, 97.7 to 100.0). Fluoroquinolone resistance-associated mutations in parC targeted by the assay were more prevalent among the Australian cohort (23.4% [95% CI,16.3 to 31.8]) compared to the Spanish population (8.8% [95% CI, 2.9% to 19.3%]) (P = 0.019). The MG+parC (beta) kit is a simple and reliable method for simultaneous detection of and fluoroquinolone resistance-associated mutations in clinical settings. This novel diagnostic tool may extend the utility of the second line of antimicrobial therapies in infection.Universitat Autònoma de Barcelona 22019-01-0120192019-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/226588https://dx.doi.org/urn:doi:10.1128/JCM.00886-19reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2265882026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Multicenter Clinical Evaluation of a Novel Multiplex Real-Time PCR (qPCR) Assay for Detection of Fluoroquinolone Resistance in |
| title |
Multicenter Clinical Evaluation of a Novel Multiplex Real-Time PCR (qPCR) Assay for Detection of Fluoroquinolone Resistance in |
| spellingShingle |
Multicenter Clinical Evaluation of a Novel Multiplex Real-Time PCR (qPCR) Assay for Detection of Fluoroquinolone Resistance in Fernández-Huerta, Miguel|||0000-0002-1733-0925 Mycoplasma genitalium Fluoroquinolone resistance Multiplex qPCR assay Multicenter evaluation Antibiotic resistance |
| title_short |
Multicenter Clinical Evaluation of a Novel Multiplex Real-Time PCR (qPCR) Assay for Detection of Fluoroquinolone Resistance in |
| title_full |
Multicenter Clinical Evaluation of a Novel Multiplex Real-Time PCR (qPCR) Assay for Detection of Fluoroquinolone Resistance in |
| title_fullStr |
Multicenter Clinical Evaluation of a Novel Multiplex Real-Time PCR (qPCR) Assay for Detection of Fluoroquinolone Resistance in |
| title_full_unstemmed |
Multicenter Clinical Evaluation of a Novel Multiplex Real-Time PCR (qPCR) Assay for Detection of Fluoroquinolone Resistance in |
| title_sort |
Multicenter Clinical Evaluation of a Novel Multiplex Real-Time PCR (qPCR) Assay for Detection of Fluoroquinolone Resistance in |
| dc.creator.none.fl_str_mv |
Fernández-Huerta, Miguel|||0000-0002-1733-0925 Bodiyabadu, Kaveesha Esperalba, Juliana|||0000-0003-1326-1341 Bradshaw, Catriona S. Serra-Pladevall, Judit|||0000-0002-4798-2385 Garland, Suzanne M. Fernandez-Naval, Candela|||0000-0002-5584-3537 Jensen, Jorgen S.|||0000-0002-7464-7435 Pumarola Suñé, Tomàs|||0000-0002-5171-7461 Ebeyan, Samantha Lundgren, Marie Tan, Lit Yeen Espasa, Mateu|||0000-0003-4822-1024 Murray, Gerald L. |
| author |
Fernández-Huerta, Miguel|||0000-0002-1733-0925 |
| author_facet |
Fernández-Huerta, Miguel|||0000-0002-1733-0925 Bodiyabadu, Kaveesha Esperalba, Juliana|||0000-0003-1326-1341 Bradshaw, Catriona S. Serra-Pladevall, Judit|||0000-0002-4798-2385 Garland, Suzanne M. Fernandez-Naval, Candela|||0000-0002-5584-3537 Jensen, Jorgen S.|||0000-0002-7464-7435 Pumarola Suñé, Tomàs|||0000-0002-5171-7461 Ebeyan, Samantha Lundgren, Marie Tan, Lit Yeen Espasa, Mateu|||0000-0003-4822-1024 Murray, Gerald L. |
| author_role |
author |
| author2 |
Bodiyabadu, Kaveesha Esperalba, Juliana|||0000-0003-1326-1341 Bradshaw, Catriona S. Serra-Pladevall, Judit|||0000-0002-4798-2385 Garland, Suzanne M. Fernandez-Naval, Candela|||0000-0002-5584-3537 Jensen, Jorgen S.|||0000-0002-7464-7435 Pumarola Suñé, Tomàs|||0000-0002-5171-7461 Ebeyan, Samantha Lundgren, Marie Tan, Lit Yeen Espasa, Mateu|||0000-0003-4822-1024 Murray, Gerald L. |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universitat Autònoma de Barcelona |
| dc.subject.none.fl_str_mv |
Mycoplasma genitalium Fluoroquinolone resistance Multiplex qPCR assay Multicenter evaluation Antibiotic resistance |
| topic |
Mycoplasma genitalium Fluoroquinolone resistance Multiplex qPCR assay Multicenter evaluation Antibiotic resistance |
| description |
causes a common sexually transmitted infection with a marked propensity to develop antimicrobial resistance. As few treatment options exist, this poses significant challenges to clinicians. Recent diagnostic advances have resulted in tests that report the simultaneous detection of and any resistance to macrolides, the first-line treatment. This allows for therapy to be tailored to the individual, thereby optimizing treatment outcomes. causes a common sexually transmitted infection with a marked propensity to develop antimicrobial resistance. As few treatment options exist, this poses significant challenges to clinicians. Recent diagnostic advances have resulted in tests that report the simultaneous detection of and any resistance to macrolides, the first-line treatment. This allows for therapy to be tailored to the individual, thereby optimizing treatment outcomes. However, resistance to fluoroquinolones, the second-line treatment, is increasing in . In this study, we describe a new assay, MG+parC (beta), which simultaneously reports the detection of and five parC mutations that have been associated with resistance to fluoroquinolones. These mutations affect the amino acid sequence of ParC at residues S83R (A247C), S83I (G248T), D87N (G259A), D87Y (G259T), and D87H (G259C). The study tested the MG+parC (beta) assay with 202 -positive clinical samples from Australia (n = 141) and Spain (n = 61). Compared to Sanger sequencing, the assay performed with a kappa value of 0.985 (95% confidence interval [CI], 0.955 to 1.000), with a mutation detection sensitivity of 97.6% (95% CI, 87.4 to 99.9), and specificity of 100.0% (95% CI, 97.7 to 100.0). Fluoroquinolone resistance-associated mutations in parC targeted by the assay were more prevalent among the Australian cohort (23.4% [95% CI,16.3 to 31.8]) compared to the Spanish population (8.8% [95% CI, 2.9% to 19.3%]) (P = 0.019). The MG+parC (beta) kit is a simple and reliable method for simultaneous detection of and fluoroquinolone resistance-associated mutations in clinical settings. This novel diagnostic tool may extend the utility of the second line of antimicrobial therapies in infection. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2 2019-01-01 2019 2019-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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https://ddd.uab.cat/record/226588 https://dx.doi.org/urn:doi:10.1128/JCM.00886-19 |
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https://ddd.uab.cat/record/226588 https://dx.doi.org/urn:doi:10.1128/JCM.00886-19 |
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Inglés eng |
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Inglés |
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eng |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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