Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration

We present a method for using long-term organotypic slice co-cultures of the entorhino-hippocampal formation to analyze the axon-regenerative properties of a determined compound. The culture method is based on the membrane interphase method, which is easy to perform and is generally reproducible. Th...

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Detalhes bibliográficos
Autores: Río Fernández, José Antonio del, Soriano García, Eduardo
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2009
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/47224
Acesso em linha:https://hdl.handle.net/2445/47224
Access Level:acceso abierto
Palavra-chave:Neurobiologia
Regeneració del sistema nerviós
Hipocamp (Cervell)
Neurones
Neurobiology
Nervous system regeneration
Hippocampus (Brain)
Neurons
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spelling Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regenerationRío Fernández, José Antonio delSoriano García, EduardoNeurobiologiaRegeneració del sistema nerviósHipocamp (Cervell)NeuronesNeurobiologyNervous system regenerationHippocampus (Brain)NeuronsWe present a method for using long-term organotypic slice co-cultures of the entorhino-hippocampal formation to analyze the axon-regenerative properties of a determined compound. The culture method is based on the membrane interphase method, which is easy to perform and is generally reproducible. The degree of axonal regeneration after treatment in lesioned cultures can be seen directly using green fluorescent protein (GFP) transgenic mice or by axon tracing and histological methods. Possible changes in cell morphology after pharmacological treatment can be determined easily by focal in vitro electroporation. The well-preserved cytoarchitectonics in the co-culture facilitate the analysis of identified cells or regenerating axons. The protocol takes up to a month.Nature Publishing Group2013201320092013info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion30 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/47224Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: http://dx.doi.org/10.1038/nprot.2009.202Nature Protocols, 2009, vol. 5, num. 2, p. 217-226http://dx.doi.org/10.1038/nprot.2009.202(c) Río Fernández, José Antonio del et al., 2009info:eu-repo/semantics/openAccessoai:recercat.cat:2445/472242026-05-29T05:05:01Z
dc.title.none.fl_str_mv Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration
title Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration
spellingShingle Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration
Río Fernández, José Antonio del
Neurobiologia
Regeneració del sistema nerviós
Hipocamp (Cervell)
Neurones
Neurobiology
Nervous system regeneration
Hippocampus (Brain)
Neurons
title_short Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration
title_full Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration
title_fullStr Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration
title_full_unstemmed Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration
title_sort Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration
dc.creator.none.fl_str_mv Río Fernández, José Antonio del
Soriano García, Eduardo
author Río Fernández, José Antonio del
author_facet Río Fernández, José Antonio del
Soriano García, Eduardo
author_role author
author2 Soriano García, Eduardo
author2_role author
dc.subject.none.fl_str_mv Neurobiologia
Regeneració del sistema nerviós
Hipocamp (Cervell)
Neurones
Neurobiology
Nervous system regeneration
Hippocampus (Brain)
Neurons
topic Neurobiologia
Regeneració del sistema nerviós
Hipocamp (Cervell)
Neurones
Neurobiology
Nervous system regeneration
Hippocampus (Brain)
Neurons
description We present a method for using long-term organotypic slice co-cultures of the entorhino-hippocampal formation to analyze the axon-regenerative properties of a determined compound. The culture method is based on the membrane interphase method, which is easy to perform and is generally reproducible. The degree of axonal regeneration after treatment in lesioned cultures can be seen directly using green fluorescent protein (GFP) transgenic mice or by axon tracing and histological methods. Possible changes in cell morphology after pharmacological treatment can be determined easily by focal in vitro electroporation. The well-preserved cytoarchitectonics in the co-culture facilitate the analysis of identified cells or regenerating axons. The protocol takes up to a month.
publishDate 2009
dc.date.none.fl_str_mv 2009
2013
2013
2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/47224
url https://hdl.handle.net/2445/47224
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: http://dx.doi.org/10.1038/nprot.2009.202
Nature Protocols, 2009, vol. 5, num. 2, p. 217-226
http://dx.doi.org/10.1038/nprot.2009.202
dc.rights.none.fl_str_mv (c) Río Fernández, José Antonio del et al., 2009
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Río Fernández, José Antonio del et al., 2009
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 30 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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