Single mutations change the Phage resistance profile in the soil bacteria Sinorhizobium meliloti
Students have learned basic microbiology techniques to understand the importance of viruses and their antiviral counterparts present in bacterial genomes. As in previous years, our system of study is a defense-associated reverse transcriptase (DRT) antiviral system (UG5 group) found in the bacteria...
| Autores: | , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/418205 |
| Acceso en línea: | http://hdl.handle.net/10261/418205 |
| Access Level: | acceso abierto |
| Palabra clave: | Sinorhizobium meliloti Bacterial conjugation Defense-associated Reverse Transcriptases (DRTs) Nitrilase domain Phage predation |
| Sumario: | Students have learned basic microbiology techniques to understand the importance of viruses and their antiviral counterparts present in bacterial genomes. As in previous years, our system of study is a defense-associated reverse transcriptase (DRT) antiviral system (UG5 group) found in the bacteria Sinorhizobium meliloti. Students demonstrate that single but critical mutations in this DRT system completely change the resistance phenotype against the phage 3.2 of S. meliloti. The presence of the defense system generates a drop of 104 fold of phage title. Mutants either in the RT domain (yvaa 296-7) or Nitrilase domain (c876a) disrupt this function and the phage can replicate. Results obtained exemplified how single mutations can have a dramatic effect on the biology of a bacteria. |
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