Micron-sized DNA-gelatin coacervates generated by ionic complexation in the presence of a nonionic polysaccharide

DNA-protein coacervate microparticles can be formed via ionic complexation between DNA and a protein, facilitated by the presence of a nonionic polymer. Despite recent advances in understanding membraneless organelles (MLOs) in eukaryotic cells, their formation through liquid-liquid phase separation...

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Detalles Bibliográficos
Autores: Vílchez, Susana, Miras, Jonathan, Farfan, S., Tur-Guasch, R., de Oliveira, N., Pérez-Calm, A., Grijalvo, Santiago, Rodríguez-Abreu, Carlos, Esquena, Jordi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/418816
Acceso en línea:http://hdl.handle.net/10261/418816
https://api.elsevier.com/content/abstract/scopus_id/105029603432
Access Level:acceso abierto
Palabra clave:Microgels
Coacervates
DNA
Dextran
Gelatin
Liquid-liquid phase separation
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Descripción
Sumario:DNA-protein coacervate microparticles can be formed via ionic complexation between DNA and a protein, facilitated by the presence of a nonionic polymer. Despite recent advances in understanding membraneless organelles (MLOs) in eukaryotic cells, their formation through liquid-liquid phase separation remains incompletely elucidated. We hypothesized that due to their opposite charges, DNA and gelatin readily form micron-sized coacervates, and particle formation is facilitated by adding a polymer immiscible with gelatin.