Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.

Human pluripotent stem cells (hPSCs) are widely used to study cardiovascular cell differentiation and function. Here, we induced differentiation of hPSCs (both embryonic and induced) to proepicardial/epicardial progenitor cells that cover the heart during development. Addition of retinoic acid (RA)...

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Autores: Guadix, Juan Antonio, Orlova, Valeria V, Giacomelli, Elisa, Bellin, Milena, Ribeiro, Marcelo C, Mummery, Christine L, Pérez-Pomares, José M, Passier, Robert
Formato: artículo
Fecha de publicación:2017
País:España
Recursos:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/17347
Acesso em linha:http://hdl.handle.net/20.500.12105/17347
Access Level:acceso abierto
Palavra-chave:cardiovascular
differentiation
human pluripotent stem cells
proepicardium
progenitor cells
Animals
Bone Morphogenetic Protein 4
Cardiovascular System
Cell Adhesion
Cell Culture Techniques
Cell Differentiation
Chick Embryo
Embryonic Development
Gene Expression Regulation, Developmental
Heart
Humans
Induced Pluripotent Stem Cells
Myocardium
Myocytes, Cardiac
Pericardium
Receptor, Platelet-Derived Growth Factor alpha
Stem Cells
Tretinoin
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spelling Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.Guadix, Juan AntonioOrlova, Valeria VGiacomelli, ElisaBellin, MilenaRibeiro, Marcelo CMummery, Christine LPérez-Pomares, José MPassier, Robertcardiovasculardifferentiationhuman pluripotent stem cellsproepicardiumprogenitor cellsAnimalsBone Morphogenetic Protein 4Cardiovascular SystemCell AdhesionCell Culture TechniquesCell DifferentiationChick EmbryoEmbryonic DevelopmentGene Expression Regulation, DevelopmentalHeartHumansInduced Pluripotent Stem CellsMyocardiumMyocytes, CardiacPericardiumReceptor, Platelet-Derived Growth Factor alphaStem CellsTretinoinHuman pluripotent stem cells (hPSCs) are widely used to study cardiovascular cell differentiation and function. Here, we induced differentiation of hPSCs (both embryonic and induced) to proepicardial/epicardial progenitor cells that cover the heart during development. Addition of retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) promoted expression of the mesodermal marker PDGFRα, upregulated characteristic (pro)epicardial progenitor cell genes, and downregulated transcription of myocardial genes. We confirmed the (pro)epicardial-like properties of these cells using in vitro co-culture assays and in ovo grafting of hPSC-epicardial cells into chick embryos. Our data show that RA + BMP4-treated hPSCs differentiate into (pro)epicardial-like cells displaying functional properties (adhesion and spreading over the myocardium) of their in vivo counterpart. The results extend evidence that hPSCs are an excellent model to study (pro)epicardial differentiation into cardiovascular cells in human development and evaluate their potential for cardiac regeneration.20242024-01-2320172017-11-2220172017-11-22research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://hdl.handle.net/20.500.12105/17347reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/173472026-06-12T12:43:37Z
dc.title.none.fl_str_mv Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.
title Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.
spellingShingle Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.
Guadix, Juan Antonio
cardiovascular
differentiation
human pluripotent stem cells
proepicardium
progenitor cells
Animals
Bone Morphogenetic Protein 4
Cardiovascular System
Cell Adhesion
Cell Culture Techniques
Cell Differentiation
Chick Embryo
Embryonic Development
Gene Expression Regulation, Developmental
Heart
Humans
Induced Pluripotent Stem Cells
Myocardium
Myocytes, Cardiac
Pericardium
Receptor, Platelet-Derived Growth Factor alpha
Stem Cells
Tretinoin
title_short Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.
title_full Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.
title_fullStr Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.
title_full_unstemmed Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.
title_sort Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.
dc.creator.none.fl_str_mv Guadix, Juan Antonio
Orlova, Valeria V
Giacomelli, Elisa
Bellin, Milena
Ribeiro, Marcelo C
Mummery, Christine L
Pérez-Pomares, José M
Passier, Robert
author Guadix, Juan Antonio
author_facet Guadix, Juan Antonio
Orlova, Valeria V
Giacomelli, Elisa
Bellin, Milena
Ribeiro, Marcelo C
Mummery, Christine L
Pérez-Pomares, José M
Passier, Robert
author_role author
author2 Orlova, Valeria V
Giacomelli, Elisa
Bellin, Milena
Ribeiro, Marcelo C
Mummery, Christine L
Pérez-Pomares, José M
Passier, Robert
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv cardiovascular
differentiation
human pluripotent stem cells
proepicardium
progenitor cells
Animals
Bone Morphogenetic Protein 4
Cardiovascular System
Cell Adhesion
Cell Culture Techniques
Cell Differentiation
Chick Embryo
Embryonic Development
Gene Expression Regulation, Developmental
Heart
Humans
Induced Pluripotent Stem Cells
Myocardium
Myocytes, Cardiac
Pericardium
Receptor, Platelet-Derived Growth Factor alpha
Stem Cells
Tretinoin
topic cardiovascular
differentiation
human pluripotent stem cells
proepicardium
progenitor cells
Animals
Bone Morphogenetic Protein 4
Cardiovascular System
Cell Adhesion
Cell Culture Techniques
Cell Differentiation
Chick Embryo
Embryonic Development
Gene Expression Regulation, Developmental
Heart
Humans
Induced Pluripotent Stem Cells
Myocardium
Myocytes, Cardiac
Pericardium
Receptor, Platelet-Derived Growth Factor alpha
Stem Cells
Tretinoin
description Human pluripotent stem cells (hPSCs) are widely used to study cardiovascular cell differentiation and function. Here, we induced differentiation of hPSCs (both embryonic and induced) to proepicardial/epicardial progenitor cells that cover the heart during development. Addition of retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) promoted expression of the mesodermal marker PDGFRα, upregulated characteristic (pro)epicardial progenitor cell genes, and downregulated transcription of myocardial genes. We confirmed the (pro)epicardial-like properties of these cells using in vitro co-culture assays and in ovo grafting of hPSC-epicardial cells into chick embryos. Our data show that RA + BMP4-treated hPSCs differentiate into (pro)epicardial-like cells displaying functional properties (adhesion and spreading over the myocardium) of their in vivo counterpart. The results extend evidence that hPSCs are an excellent model to study (pro)epicardial differentiation into cardiovascular cells in human development and evaluate their potential for cardiac regeneration.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-11-22
2017
2017-11-22
2024
2024-01-23
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/17347
url http://hdl.handle.net/20.500.12105/17347
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.198674

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