Interlaboratory comparison of microplastic extraction methods from marine biota tissues: A harmonization exercise of the Plastic Busters MPAs project

In the framework of the Plastic Busters MPAs project, a harmonization exercise on two methods of microplastic extraction from biological samples i.e. 15% H2O2 digestion and 10% KOH digestion was carried out. The two methods were tested in four laboratories on fish gastrointestinal tracts and mussel...

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Autores: Tsangaris, Catherine, Panti, Cristina, Compa, Montserrat, Pedà, Cristina, Digka, Nikoletta, Baini, Matteo, D’Alessandro, Michela, Alomar, Carme, Patsiou, Danae, Giani, Dario, Romeo, Teresa, Deudero, Salud
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::f686b37d23820976d4ee45fa03f74b31
Acceso en línea:http://hdl.handle.net/10261/317742
Access Level:acceso abierto
Palabra clave:Microplastic
Medio Marino
Centro Oceanográfico de Baleares
Ingestion
Isolation
Protocol harmonization
Mullus barbatus
Mytilus galloprovincialis
fish
marine pollution
biota
pollution
rights
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spelling Interlaboratory comparison of microplastic extraction methods from marine biota tissues: A harmonization exercise of the Plastic Busters MPAs projectTsangaris, CatherinePanti, CristinaCompa, MontserratPedà, CristinaDigka, NikolettaBaini, MatteoD’Alessandro, MichelaAlomar, CarmePatsiou, DanaeGiani, DarioRomeo, TeresaDeudero, SaludMicroplasticMedio MarinoCentro Oceanográfico de BalearesIngestionIsolationProtocol harmonizationMullus barbatusMytilus galloprovincialisfishmarine pollutionbiotapollutionrightsIn the framework of the Plastic Busters MPAs project, a harmonization exercise on two methods of microplastic extraction from biological samples i.e. 15% H2O2 digestion and 10% KOH digestion was carried out. The two methods were tested in four laboratories on fish gastrointestinal tracts and mussel tissues spiked with polyethylene, polypropylene and polyethylene terephthalate. The recovery percentage of microplastics for each method, species and polymer tested were overall similar among laboratories, and interlaboratory coefficient of variation was less than 11% for the majority of samples. Microplastic recovery rates for the two methods were similar for each sample tested, but overall mean interlaboratory recovery rate using KOH (96.67%) was higher than H2O2 (88.75%). Results validate the use of both methods for extracting microplastics from biota tissues. However, when comparing the two methods in terms of microplastic recovery rate, time consumed, technical difficulties and cost, digestion with 10% KOH is considered optimal.SI202320232021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/317742reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCentro Oceanográfico de Balearesinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::f686b37d23820976d4ee45fa03f74b312026-05-22T06:33:51Z
dc.title.none.fl_str_mv Interlaboratory comparison of microplastic extraction methods from marine biota tissues: A harmonization exercise of the Plastic Busters MPAs project
title Interlaboratory comparison of microplastic extraction methods from marine biota tissues: A harmonization exercise of the Plastic Busters MPAs project
spellingShingle Interlaboratory comparison of microplastic extraction methods from marine biota tissues: A harmonization exercise of the Plastic Busters MPAs project
Tsangaris, Catherine
Microplastic
Medio Marino
Centro Oceanográfico de Baleares
Ingestion
Isolation
Protocol harmonization
Mullus barbatus
Mytilus galloprovincialis
fish
marine pollution
biota
pollution
rights
title_short Interlaboratory comparison of microplastic extraction methods from marine biota tissues: A harmonization exercise of the Plastic Busters MPAs project
title_full Interlaboratory comparison of microplastic extraction methods from marine biota tissues: A harmonization exercise of the Plastic Busters MPAs project
title_fullStr Interlaboratory comparison of microplastic extraction methods from marine biota tissues: A harmonization exercise of the Plastic Busters MPAs project
title_full_unstemmed Interlaboratory comparison of microplastic extraction methods from marine biota tissues: A harmonization exercise of the Plastic Busters MPAs project
title_sort Interlaboratory comparison of microplastic extraction methods from marine biota tissues: A harmonization exercise of the Plastic Busters MPAs project
dc.creator.none.fl_str_mv Tsangaris, Catherine
Panti, Cristina
Compa, Montserrat
Pedà, Cristina
Digka, Nikoletta
Baini, Matteo
D’Alessandro, Michela
Alomar, Carme
Patsiou, Danae
Giani, Dario
Romeo, Teresa
Deudero, Salud
author Tsangaris, Catherine
author_facet Tsangaris, Catherine
Panti, Cristina
Compa, Montserrat
Pedà, Cristina
Digka, Nikoletta
Baini, Matteo
D’Alessandro, Michela
Alomar, Carme
Patsiou, Danae
Giani, Dario
Romeo, Teresa
Deudero, Salud
author_role author
author2 Panti, Cristina
Compa, Montserrat
Pedà, Cristina
Digka, Nikoletta
Baini, Matteo
D’Alessandro, Michela
Alomar, Carme
Patsiou, Danae
Giani, Dario
Romeo, Teresa
Deudero, Salud
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Microplastic
Medio Marino
Centro Oceanográfico de Baleares
Ingestion
Isolation
Protocol harmonization
Mullus barbatus
Mytilus galloprovincialis
fish
marine pollution
biota
pollution
rights
topic Microplastic
Medio Marino
Centro Oceanográfico de Baleares
Ingestion
Isolation
Protocol harmonization
Mullus barbatus
Mytilus galloprovincialis
fish
marine pollution
biota
pollution
rights
description In the framework of the Plastic Busters MPAs project, a harmonization exercise on two methods of microplastic extraction from biological samples i.e. 15% H2O2 digestion and 10% KOH digestion was carried out. The two methods were tested in four laboratories on fish gastrointestinal tracts and mussel tissues spiked with polyethylene, polypropylene and polyethylene terephthalate. The recovery percentage of microplastics for each method, species and polymer tested were overall similar among laboratories, and interlaboratory coefficient of variation was less than 11% for the majority of samples. Microplastic recovery rates for the two methods were similar for each sample tested, but overall mean interlaboratory recovery rate using KOH (96.67%) was higher than H2O2 (88.75%). Results validate the use of both methods for extracting microplastics from biota tissues. However, when comparing the two methods in terms of microplastic recovery rate, time consumed, technical difficulties and cost, digestion with 10% KOH is considered optimal.
publishDate 2021
dc.date.none.fl_str_mv 2021
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/317742
url http://hdl.handle.net/10261/317742
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Centro Oceanográfico de Baleares
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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