Assessment of toxicity and endocrine-disrupting activity of bisphenol analogues during ozone and UV treatments in zebrafish eleutheroembryos

In recent decades, increased regulations on the use of bisphenol A (BPA) have prompted a surge in the use of BPA alternatives. Consequently, a widespread occurrence of BPA substitutes in aquatic environments is currently being detected. While some evidence exists about the degradation of these compo...

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Autores: Porcar-Santos, Oriol, Sanz, Claudia, Cruz-Alcalde, Alberto, Lima, Tugstenio, Gual, Marta, Navarro-Martín, Laia, Sans, Carmen
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/373413
Acceso en línea:http://hdl.handle.net/10261/373413
https://api.elsevier.com/content/abstract/scopus_id/85210133786
Access Level:acceso abierto
Palabra clave:Zebrafish eleutheroembryos
Bisphenol A substitutes
Endocrine disruption
Estrogenicity
Ozone
UV-C radiation
http://metadata.un.org/sdg/14
http://metadata.un.org/sdg/6
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Ensure availability and sustainable management of water and sanitation for all
Ensure sustainable consumption and production patterns
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
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spelling Assessment of toxicity and endocrine-disrupting activity of bisphenol analogues during ozone and UV treatments in zebrafish eleutheroembryosPorcar-Santos, OriolSanz, ClaudiaCruz-Alcalde, AlbertoLima, TugstenioGual, MartaNavarro-Martín, LaiaSans, CarmenZebrafish eleutheroembryosBisphenol A substitutesEndocrine disruptionEstrogenicityOzoneUV-C radiationhttp://metadata.un.org/sdg/14http://metadata.un.org/sdg/6http://metadata.un.org/sdg/3Ensure healthy lives and promote well-being for all at all agesEnsure availability and sustainable management of water and sanitation for allEnsure sustainable consumption and production patternsConserve and sustainably use the oceans, seas and marine resources for sustainable developmentIn recent decades, increased regulations on the use of bisphenol A (BPA) have prompted a surge in the use of BPA alternatives. Consequently, a widespread occurrence of BPA substitutes in aquatic environments is currently being detected. While some evidence exists about the degradation of these compounds through various water treatment technologies, the evolution of the resulting toxicity and endocrine-disrupting activity during these processes remains scarcely evaluated. In this study, the acute toxicity and transcriptomic responses in zebrafish eleutheroembryos exposed to selected bisphenols (BPA, bisphenol AF (BPAF) and bisphenol CCl (BPC-Cl)) were assessed during their oxidation by ozone. In addition, the response of zebrafish eleutheroembryos exposed to BPC-Cl treated with UV radiation was also investigated. Results showed that both ozonation and UV treatment effectively reduced the intrinsic toxicity of the studied bisphenols. This was observed with the increase of the survival and swim bladder inflation rates of zebrafish eleutheroembryos, reaching control levels. In concordance with these results, the initially altered mRNA levels in genes related to xenobiotic stimulus (cyp2k18); lipid homeostasis and transport (apoa1a); retinoid metabolism (aldh1a2); neutrophil differentiation (alas1); and oxygen transport (hbae3) in zebrafish eleutheroembryos were generally mitigated during the ozonation and UV treatment of bisphenols. Similarly, the high estrogenicity of these bisphenols, observed by elevated mRNA levels of cyp19a1b, decreased significantly during the ozonation treatment, reaching control levels. On the contrary, an increase in mRNA levels of fads2 and cyp19a1b was observed in animals exposed to BPC-Cl treated with UV radiation. These results suggest that the photolysis products of BPC-Cl may induce disruption of the lipid biosynthesis and estrogenicity. This was further confirmed with RNA-sequencing analysis, which revealed that embryos exposed to BPC-Cl treated with UV radiation presented alterations in mRNA levels of genes specifically related to estrogenic stimulus.This work was financially supported by MCIN/AEI/10.13039/501100011033, with the project PID2020-112674RB-I00, the project RYC2019-026426-I and Oriol Porcar's FPI fellowship (PRE2018-084184)-FSE.Peer reviewedElsevierMinisterio de Ciencia y Tecnología (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/373413https://api.elsevier.com/content/abstract/scopus_id/85210133786reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MCIN/AEI/10.13039The Science of the total environmenthttps://doi.org/10.1016/j.scitotenv.2024.177591Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3734132026-05-22T06:33:51Z
dc.title.none.fl_str_mv Assessment of toxicity and endocrine-disrupting activity of bisphenol analogues during ozone and UV treatments in zebrafish eleutheroembryos
title Assessment of toxicity and endocrine-disrupting activity of bisphenol analogues during ozone and UV treatments in zebrafish eleutheroembryos
spellingShingle Assessment of toxicity and endocrine-disrupting activity of bisphenol analogues during ozone and UV treatments in zebrafish eleutheroembryos
Porcar-Santos, Oriol
Zebrafish eleutheroembryos
Bisphenol A substitutes
Endocrine disruption
Estrogenicity
Ozone
UV-C radiation
http://metadata.un.org/sdg/14
http://metadata.un.org/sdg/6
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Ensure availability and sustainable management of water and sanitation for all
Ensure sustainable consumption and production patterns
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
title_short Assessment of toxicity and endocrine-disrupting activity of bisphenol analogues during ozone and UV treatments in zebrafish eleutheroembryos
title_full Assessment of toxicity and endocrine-disrupting activity of bisphenol analogues during ozone and UV treatments in zebrafish eleutheroembryos
title_fullStr Assessment of toxicity and endocrine-disrupting activity of bisphenol analogues during ozone and UV treatments in zebrafish eleutheroembryos
title_full_unstemmed Assessment of toxicity and endocrine-disrupting activity of bisphenol analogues during ozone and UV treatments in zebrafish eleutheroembryos
title_sort Assessment of toxicity and endocrine-disrupting activity of bisphenol analogues during ozone and UV treatments in zebrafish eleutheroembryos
dc.creator.none.fl_str_mv Porcar-Santos, Oriol
Sanz, Claudia
Cruz-Alcalde, Alberto
Lima, Tugstenio
Gual, Marta
Navarro-Martín, Laia
Sans, Carmen
author Porcar-Santos, Oriol
author_facet Porcar-Santos, Oriol
Sanz, Claudia
Cruz-Alcalde, Alberto
Lima, Tugstenio
Gual, Marta
Navarro-Martín, Laia
Sans, Carmen
author_role author
author2 Sanz, Claudia
Cruz-Alcalde, Alberto
Lima, Tugstenio
Gual, Marta
Navarro-Martín, Laia
Sans, Carmen
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia y Tecnología (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Zebrafish eleutheroembryos
Bisphenol A substitutes
Endocrine disruption
Estrogenicity
Ozone
UV-C radiation
http://metadata.un.org/sdg/14
http://metadata.un.org/sdg/6
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Ensure availability and sustainable management of water and sanitation for all
Ensure sustainable consumption and production patterns
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
topic Zebrafish eleutheroembryos
Bisphenol A substitutes
Endocrine disruption
Estrogenicity
Ozone
UV-C radiation
http://metadata.un.org/sdg/14
http://metadata.un.org/sdg/6
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Ensure availability and sustainable management of water and sanitation for all
Ensure sustainable consumption and production patterns
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
description In recent decades, increased regulations on the use of bisphenol A (BPA) have prompted a surge in the use of BPA alternatives. Consequently, a widespread occurrence of BPA substitutes in aquatic environments is currently being detected. While some evidence exists about the degradation of these compounds through various water treatment technologies, the evolution of the resulting toxicity and endocrine-disrupting activity during these processes remains scarcely evaluated. In this study, the acute toxicity and transcriptomic responses in zebrafish eleutheroembryos exposed to selected bisphenols (BPA, bisphenol AF (BPAF) and bisphenol CCl (BPC-Cl)) were assessed during their oxidation by ozone. In addition, the response of zebrafish eleutheroembryos exposed to BPC-Cl treated with UV radiation was also investigated. Results showed that both ozonation and UV treatment effectively reduced the intrinsic toxicity of the studied bisphenols. This was observed with the increase of the survival and swim bladder inflation rates of zebrafish eleutheroembryos, reaching control levels. In concordance with these results, the initially altered mRNA levels in genes related to xenobiotic stimulus (cyp2k18); lipid homeostasis and transport (apoa1a); retinoid metabolism (aldh1a2); neutrophil differentiation (alas1); and oxygen transport (hbae3) in zebrafish eleutheroembryos were generally mitigated during the ozonation and UV treatment of bisphenols. Similarly, the high estrogenicity of these bisphenols, observed by elevated mRNA levels of cyp19a1b, decreased significantly during the ozonation treatment, reaching control levels. On the contrary, an increase in mRNA levels of fads2 and cyp19a1b was observed in animals exposed to BPC-Cl treated with UV radiation. These results suggest that the photolysis products of BPC-Cl may induce disruption of the lipid biosynthesis and estrogenicity. This was further confirmed with RNA-sequencing analysis, which revealed that embryos exposed to BPC-Cl treated with UV radiation presented alterations in mRNA levels of genes specifically related to estrogenic stimulus.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/373413
https://api.elsevier.com/content/abstract/scopus_id/85210133786
url http://hdl.handle.net/10261/373413
https://api.elsevier.com/content/abstract/scopus_id/85210133786
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MCIN/AEI/10.13039
The Science of the total environment
https://doi.org/10.1016/j.scitotenv.2024.177591
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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