MicroRNAs control the apoptotic threshold in primed pluripotent stem cells through regulation of BIM

Mammalian primed pluripotent stem cells have been shown to be highly susceptible to cell death stimuli due to their low apoptotic threshold, but how this threshold is regulated remains largely unknown. Here we identify microRNA (miRNA)-mediated regulation as a key mechanism controlling apoptosis in...

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Detalles Bibliográficos
Autores: Pernaute, Barbara, Spruce, Thomas, Smith, Kimberley M, Sánchez-Nieto, Juan Miguel, Manzanares, Miguel, Cobb, Bradley, Rodríguez, Tristan A
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/7353
Acceso en línea:http://hdl.handle.net/20.500.12105/7353
Access Level:acceso abierto
Palabra clave:Animals
Apoptosis
Apoptosis Regulatory Proteins
Bcl-2-Like Protein 11
Cell Survival
Cells, Cultured
Gene Expression Profiling
Membrane Proteins
Mice
MicroRNAs
Mitochondria
Pluripotent Stem Cells
Proto-Oncogene Proteins
Gene Expression Regulation, Developmental
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spelling MicroRNAs control the apoptotic threshold in primed pluripotent stem cells through regulation of BIMPernaute, BarbaraSpruce, ThomasSmith, Kimberley MSánchez-Nieto, Juan MiguelManzanares, MiguelCobb, BradleyRodríguez, Tristan AAnimalsApoptosisApoptosis Regulatory ProteinsBcl-2-Like Protein 11Cell SurvivalCells, CulturedGene Expression ProfilingMembrane ProteinsMiceMicroRNAsMitochondriaPluripotent Stem CellsProto-Oncogene ProteinsGene Expression Regulation, DevelopmentalMammalian primed pluripotent stem cells have been shown to be highly susceptible to cell death stimuli due to their low apoptotic threshold, but how this threshold is regulated remains largely unknown. Here we identify microRNA (miRNA)-mediated regulation as a key mechanism controlling apoptosis in the post-implantation epiblast. Moreover, we found that three miRNA families, miR-20, miR-92, and miR-302, control the mitochondrial apoptotic machinery by fine-tuning the levels of expression of the proapoptotic protein BIM. These families therefore represent an essential buffer needed to maintain cell survival in stem cells that are primed for not only differentiation but also cell death.Cold Spring Harbor Laboratory PressFundación ProCNICMedical Research Council (Reino Unido)European Molecular Biology OrganizationUnión Europea. Comisión Europea20192019-03-1820142014-09-0120142014-09-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/7353reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengEuropean Commission http://dx.doi.org/10.13039/501100000780 Seventh Framework Programme 273884open accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/73532026-06-12T12:43:37Z
dc.title.none.fl_str_mv MicroRNAs control the apoptotic threshold in primed pluripotent stem cells through regulation of BIM
title MicroRNAs control the apoptotic threshold in primed pluripotent stem cells through regulation of BIM
spellingShingle MicroRNAs control the apoptotic threshold in primed pluripotent stem cells through regulation of BIM
Pernaute, Barbara
Animals
Apoptosis
Apoptosis Regulatory Proteins
Bcl-2-Like Protein 11
Cell Survival
Cells, Cultured
Gene Expression Profiling
Membrane Proteins
Mice
MicroRNAs
Mitochondria
Pluripotent Stem Cells
Proto-Oncogene Proteins
Gene Expression Regulation, Developmental
title_short MicroRNAs control the apoptotic threshold in primed pluripotent stem cells through regulation of BIM
title_full MicroRNAs control the apoptotic threshold in primed pluripotent stem cells through regulation of BIM
title_fullStr MicroRNAs control the apoptotic threshold in primed pluripotent stem cells through regulation of BIM
title_full_unstemmed MicroRNAs control the apoptotic threshold in primed pluripotent stem cells through regulation of BIM
title_sort MicroRNAs control the apoptotic threshold in primed pluripotent stem cells through regulation of BIM
dc.creator.none.fl_str_mv Pernaute, Barbara
Spruce, Thomas
Smith, Kimberley M
Sánchez-Nieto, Juan Miguel
Manzanares, Miguel
Cobb, Bradley
Rodríguez, Tristan A
author Pernaute, Barbara
author_facet Pernaute, Barbara
Spruce, Thomas
Smith, Kimberley M
Sánchez-Nieto, Juan Miguel
Manzanares, Miguel
Cobb, Bradley
Rodríguez, Tristan A
author_role author
author2 Spruce, Thomas
Smith, Kimberley M
Sánchez-Nieto, Juan Miguel
Manzanares, Miguel
Cobb, Bradley
Rodríguez, Tristan A
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fundación ProCNIC
Medical Research Council (Reino Unido)
European Molecular Biology Organization
Unión Europea. Comisión Europea
dc.subject.none.fl_str_mv Animals
Apoptosis
Apoptosis Regulatory Proteins
Bcl-2-Like Protein 11
Cell Survival
Cells, Cultured
Gene Expression Profiling
Membrane Proteins
Mice
MicroRNAs
Mitochondria
Pluripotent Stem Cells
Proto-Oncogene Proteins
Gene Expression Regulation, Developmental
topic Animals
Apoptosis
Apoptosis Regulatory Proteins
Bcl-2-Like Protein 11
Cell Survival
Cells, Cultured
Gene Expression Profiling
Membrane Proteins
Mice
MicroRNAs
Mitochondria
Pluripotent Stem Cells
Proto-Oncogene Proteins
Gene Expression Regulation, Developmental
description Mammalian primed pluripotent stem cells have been shown to be highly susceptible to cell death stimuli due to their low apoptotic threshold, but how this threshold is regulated remains largely unknown. Here we identify microRNA (miRNA)-mediated regulation as a key mechanism controlling apoptosis in the post-implantation epiblast. Moreover, we found that three miRNA families, miR-20, miR-92, and miR-302, control the mitochondrial apoptotic machinery by fine-tuning the levels of expression of the proapoptotic protein BIM. These families therefore represent an essential buffer needed to maintain cell survival in stem cells that are primed for not only differentiation but also cell death.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-09-01
2014
2014-09-01
2019
2019-03-18
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/7353
url http://hdl.handle.net/20.500.12105/7353
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission http://dx.doi.org/10.13039/501100000780 Seventh Framework Programme 273884
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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