MicroRNAs control the apoptotic threshold in primed pluripotent stem cells through regulation of BIM
Mammalian primed pluripotent stem cells have been shown to be highly susceptible to cell death stimuli due to their low apoptotic threshold, but how this threshold is regulated remains largely unknown. Here we identify microRNA (miRNA)-mediated regulation as a key mechanism controlling apoptosis in...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2014 |
| País: | España |
| Institución: | Instituto de Salud Carlos III (ISCIII) |
| Repositorio: | Repisalud |
| Idioma: | inglés |
| OAI Identifier: | oai:repisalud.isciii.es:20.500.12105/7353 |
| Acceso en línea: | http://hdl.handle.net/20.500.12105/7353 |
| Access Level: | acceso abierto |
| Palabra clave: | Animals Apoptosis Apoptosis Regulatory Proteins Bcl-2-Like Protein 11 Cell Survival Cells, Cultured Gene Expression Profiling Membrane Proteins Mice MicroRNAs Mitochondria Pluripotent Stem Cells Proto-Oncogene Proteins Gene Expression Regulation, Developmental |
| Sumario: | Mammalian primed pluripotent stem cells have been shown to be highly susceptible to cell death stimuli due to their low apoptotic threshold, but how this threshold is regulated remains largely unknown. Here we identify microRNA (miRNA)-mediated regulation as a key mechanism controlling apoptosis in the post-implantation epiblast. Moreover, we found that three miRNA families, miR-20, miR-92, and miR-302, control the mitochondrial apoptotic machinery by fine-tuning the levels of expression of the proapoptotic protein BIM. These families therefore represent an essential buffer needed to maintain cell survival in stem cells that are primed for not only differentiation but also cell death. |
|---|