Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation

Lysyl oxidase-like 2 (LOXL2) is a copper-dependent monoamine oxidase that contributes to the remodelling of the extracellular matrix (ECM) by cross linkage of collagen and elastin fibres and has emerged as a potential therapeutic target in cancer and fibrosis. In the skin, LOXL2 is essential for epi...

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Autores: Kober, Katharina Isabelle, Cano, Amparo, Géraud, Cyrill, Sipilaä, Kalle, Mobasseri, Seyedeh Atefeh, Philippeos, Christina, Pisco, Angela Oliveira, Stannard, Andrew, Martín, Alberto, Salvador, Fernando, Santos, Vanesa, Boutros, Michael, Rognoni, Emanuel, Watt, Fiona M.
Formato: artículo
Fecha de publicación:2018
País:España
Recursos:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/685147
Acesso em linha:http://hdl.handle.net/10486/685147
https://dx.doi.org/10.1371/journal. pone.0199679
Access Level:acceso abierto
Palavra-chave:LOXL2
Dermal development
Homeostasis
Stroma of DMBA-TPA
Tumours
Medicina
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spelling Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formationKober, Katharina IsabelleCano, AmparoGéraud, CyrillSipilaä, KalleMobasseri, Seyedeh AtefehPhilippeos, ChristinaPisco, Angela OliveiraStannard, AndrewMartín, AlbertoSalvador, FernandoSantos, VanesaBoutros, MichaelRognoni, EmanuelWatt, Fiona M.LOXL2Dermal developmentHomeostasisStroma of DMBA-TPATumoursMedicinaLysyl oxidase-like 2 (LOXL2) is a copper-dependent monoamine oxidase that contributes to the remodelling of the extracellular matrix (ECM) by cross linkage of collagen and elastin fibres and has emerged as a potential therapeutic target in cancer and fibrosis. In the skin, LOXL2 is essential for epidermal cell polarity and differentiation. However, its role in the dermis has not been evaluated. We found that Loxl2 is dispensable for mouse dermal development, maturation and homeostasis, yet affects dermal stiffness. Neither loss of Loxl2 nor increased Loxl2 expression affected dermal architecture following treatment with the phor-bol ester TPA. Furthermore, Loxl2 expression did not alter the stroma of DMBA-TPA-induced tumours. We conclude that, although Loxl2 is expressed in both dermis and epidermis, its function appears largely confined to the epidermis.F.M.W. gratefully acknowledges financial support from the Medical Research Council (G1100073), Cancer Research UK (C219/A23522) and the Wellcome Trust (096540/Z/11/Z). Research in A.C.'s lab is supported by the Spanish Ministry of Economy and Innovation (SAF2016-76504-R), Instituto de Salud Carlos III (RTICC: RD12/0036/0007 and CIBERONC: CB16/12/00295) and Worldwide Cancer Research (formerly AICR) (12-1057). E.R. is the recipient of an EMBO long-term fellowship (aALTF594-2014) and an EMBO Advanced Fellowship (aALTF 523-2017). K.S. is the recipient of a Finnish Cultural Foundation Fellowship. K.I.K. was supported by the Research Training Group RTG2099 "Hallmarks of Skin Cancer" of the German Research Foundation (Deutsche Forschungsgemeinschaft /DFG) GTK2099/RT2099, project 3 (M.B.). This work was supported in part by grants from the German Research Foundation (Deutsche Forschungsgemeinschaft / DFG) GRK2099/ RTG2099, project 7 (C.G.). A.S. is supported by the Engineering and Physical Sciences Research Council (EP/K00641X/1 and EP/M022536/1)Public Library of ScienceDepartamento de BioquímicaFacultad de MedicinaInstituto de Investigaciones Biomédicas "Alberto Sols" (IIBM)20182018-06-28research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/685147https://dx.doi.org/10.1371/journal. pone.0199679reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6851472026-06-23T12:46:27Z
dc.title.none.fl_str_mv Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation
title Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation
spellingShingle Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation
Kober, Katharina Isabelle
LOXL2
Dermal development
Homeostasis
Stroma of DMBA-TPA
Tumours
Medicina
title_short Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation
title_full Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation
title_fullStr Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation
title_full_unstemmed Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation
title_sort Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation
dc.creator.none.fl_str_mv Kober, Katharina Isabelle
Cano, Amparo
Géraud, Cyrill
Sipilaä, Kalle
Mobasseri, Seyedeh Atefeh
Philippeos, Christina
Pisco, Angela Oliveira
Stannard, Andrew
Martín, Alberto
Salvador, Fernando
Santos, Vanesa
Boutros, Michael
Rognoni, Emanuel
Watt, Fiona M.
author Kober, Katharina Isabelle
author_facet Kober, Katharina Isabelle
Cano, Amparo
Géraud, Cyrill
Sipilaä, Kalle
Mobasseri, Seyedeh Atefeh
Philippeos, Christina
Pisco, Angela Oliveira
Stannard, Andrew
Martín, Alberto
Salvador, Fernando
Santos, Vanesa
Boutros, Michael
Rognoni, Emanuel
Watt, Fiona M.
author_role author
author2 Cano, Amparo
Géraud, Cyrill
Sipilaä, Kalle
Mobasseri, Seyedeh Atefeh
Philippeos, Christina
Pisco, Angela Oliveira
Stannard, Andrew
Martín, Alberto
Salvador, Fernando
Santos, Vanesa
Boutros, Michael
Rognoni, Emanuel
Watt, Fiona M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Bioquímica
Facultad de Medicina
Instituto de Investigaciones Biomédicas "Alberto Sols" (IIBM)
dc.subject.none.fl_str_mv LOXL2
Dermal development
Homeostasis
Stroma of DMBA-TPA
Tumours
Medicina
topic LOXL2
Dermal development
Homeostasis
Stroma of DMBA-TPA
Tumours
Medicina
description Lysyl oxidase-like 2 (LOXL2) is a copper-dependent monoamine oxidase that contributes to the remodelling of the extracellular matrix (ECM) by cross linkage of collagen and elastin fibres and has emerged as a potential therapeutic target in cancer and fibrosis. In the skin, LOXL2 is essential for epidermal cell polarity and differentiation. However, its role in the dermis has not been evaluated. We found that Loxl2 is dispensable for mouse dermal development, maturation and homeostasis, yet affects dermal stiffness. Neither loss of Loxl2 nor increased Loxl2 expression affected dermal architecture following treatment with the phor-bol ester TPA. Furthermore, Loxl2 expression did not alter the stroma of DMBA-TPA-induced tumours. We conclude that, although Loxl2 is expressed in both dermis and epidermis, its function appears largely confined to the epidermis.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-06-28
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/685147
https://dx.doi.org/10.1371/journal. pone.0199679
url http://hdl.handle.net/10486/685147
https://dx.doi.org/10.1371/journal. pone.0199679
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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