Promoter-driven splicing regulation in fission yeast

The meiotic cell cycle is modified from the mitotic cell cycle by having a premeiotic S phase which leads to high levels of recombination, two rounds of nuclear division with no intervening DNA synthesis, and a reductional pattern of chromosome segregation. Rem1 is a cyclin that is expressed only du...

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Detalhes bibliográficos
Autor: Moldón Vara, Alberto
Formato: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2008
País:España
Recursos:CBUC, CESCA
Repositorio:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/7125
Acesso em linha:http://www.tdx.cat/TDX-0518109-102004
http://hdl.handle.net/10803/7125
Access Level:acceso abierto
Palavra-chave:Retención intrónica
Forkhead
Fkh2
Mei4
Rem1
Inmunoprecipitación de cromatina
Recombinación
Levadura de fisión
Promotor
Ciclina
Meiosis
Schizosaccharomyces pombe
Procesamiento
Intron retention
Chromatin Immunoprecipitation
Promoter
Fission yeast
Recombination
Cyclin
Splicing
577
id ES_95df95bad4ccf1f2aebd8d647dcce0a5
oai_identifier_str oai:www.tdx.cat:10803/7125
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Promoter-driven splicing regulation in fission yeast
title Promoter-driven splicing regulation in fission yeast
spellingShingle Promoter-driven splicing regulation in fission yeast
Moldón Vara, Alberto
Retención intrónica
Forkhead
Fkh2
Mei4
Rem1
Inmunoprecipitación de cromatina
Recombinación
Levadura de fisión
Promotor
Ciclina
Meiosis
Schizosaccharomyces pombe
Procesamiento
Intron retention
Forkhead
Fkh2
Mei4
Rem1
Chromatin Immunoprecipitation
Promoter
Fission yeast
Recombination
Cyclin
Meiosis
Schizosaccharomyces pombe
Splicing
577
title_short Promoter-driven splicing regulation in fission yeast
title_full Promoter-driven splicing regulation in fission yeast
title_fullStr Promoter-driven splicing regulation in fission yeast
title_full_unstemmed Promoter-driven splicing regulation in fission yeast
title_sort Promoter-driven splicing regulation in fission yeast
dc.creator.none.fl_str_mv Moldón Vara, Alberto
author Moldón Vara, Alberto
author_facet Moldón Vara, Alberto
author_role author
dc.contributor.none.fl_str_mv Ayté del Olmo, José
Universitat Pompeu Fabra. Departament de Ciències Experimentals i de la Salut
dc.subject.none.fl_str_mv Retención intrónica
Forkhead
Fkh2
Mei4
Rem1
Inmunoprecipitación de cromatina
Recombinación
Levadura de fisión
Promotor
Ciclina
Meiosis
Schizosaccharomyces pombe
Procesamiento
Intron retention
Forkhead
Fkh2
Mei4
Rem1
Chromatin Immunoprecipitation
Promoter
Fission yeast
Recombination
Cyclin
Meiosis
Schizosaccharomyces pombe
Splicing
577
topic Retención intrónica
Forkhead
Fkh2
Mei4
Rem1
Inmunoprecipitación de cromatina
Recombinación
Levadura de fisión
Promotor
Ciclina
Meiosis
Schizosaccharomyces pombe
Procesamiento
Intron retention
Forkhead
Fkh2
Mei4
Rem1
Chromatin Immunoprecipitation
Promoter
Fission yeast
Recombination
Cyclin
Meiosis
Schizosaccharomyces pombe
Splicing
577
description The meiotic cell cycle is modified from the mitotic cell cycle by having a premeiotic S phase which leads to high levels of recombination, two rounds of nuclear division with no intervening DNA synthesis, and a reductional pattern of chromosome segregation. Rem1 is a cyclin that is expressed only during meiosis in the fission yeast Schizosaccharomyces pombe. Cells in which rem1 has been deleted show a decreased intragenic meiotic recombination and a delay at the onset of meiosis I. When ectopically expressed in mitotically growing cells, Rem1 induces a G1 arrest followed by severe mitotic catastrophes. Here we show that rem1 expression is regulated at the level of both transcription and splicing, encoding for two proteins with different function depending on the intron retention. We have determined that the regulation of rem1 splicing is not dependent on any transcribed region of the gene. Furthermore, when the rem1 promoter is fused to other intron-containing genes, the chimeras show a meiotic-specific regulation of splicing, exactly as endogenous rem1. This regulation is dependent on two transcription factors of the forkhead family, Mei4 and Fkh2. While Mei4 induces both transcription and splicing of rem1, Fkh2 is responsible for the intron retention of the transcript during vegetative growth and pre-meiotic S phase.
publishDate 2008
dc.date.none.fl_str_mv 2008
2009
2009
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://www.tdx.cat/TDX-0518109-102004
http://hdl.handle.net/10803/7125
url http://www.tdx.cat/TDX-0518109-102004
http://hdl.handle.net/10803/7125
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universitat Pompeu Fabra
publisher.none.fl_str_mv Universitat Pompeu Fabra
dc.source.none.fl_str_mv TDX (Tesis Doctorals en Xarxa)
reponame:TDR. Tesis Doctorales en Red
instname:CBUC, CESCA
instname_str CBUC, CESCA
reponame_str TDR. Tesis Doctorales en Red
collection TDR. Tesis Doctorales en Red
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869413889120141312
spelling Promoter-driven splicing regulation in fission yeastMoldón Vara, AlbertoRetención intrónicaForkheadFkh2Mei4Rem1Inmunoprecipitación de cromatinaRecombinaciónLevadura de fisiónPromotorCiclinaMeiosisSchizosaccharomyces pombeProcesamientoIntron retentionForkheadFkh2Mei4Rem1Chromatin ImmunoprecipitationPromoterFission yeastRecombinationCyclinMeiosisSchizosaccharomyces pombeSplicing577The meiotic cell cycle is modified from the mitotic cell cycle by having a premeiotic S phase which leads to high levels of recombination, two rounds of nuclear division with no intervening DNA synthesis, and a reductional pattern of chromosome segregation. Rem1 is a cyclin that is expressed only during meiosis in the fission yeast Schizosaccharomyces pombe. Cells in which rem1 has been deleted show a decreased intragenic meiotic recombination and a delay at the onset of meiosis I. When ectopically expressed in mitotically growing cells, Rem1 induces a G1 arrest followed by severe mitotic catastrophes. Here we show that rem1 expression is regulated at the level of both transcription and splicing, encoding for two proteins with different function depending on the intron retention. We have determined that the regulation of rem1 splicing is not dependent on any transcribed region of the gene. Furthermore, when the rem1 promoter is fused to other intron-containing genes, the chimeras show a meiotic-specific regulation of splicing, exactly as endogenous rem1. This regulation is dependent on two transcription factors of the forkhead family, Mei4 and Fkh2. While Mei4 induces both transcription and splicing of rem1, Fkh2 is responsible for the intron retention of the transcript during vegetative growth and pre-meiotic S phase.El ciclo meiótico se diferencia del ciclo mitótico por tener una fase S pre-meiótica caracterizada por altos niveles de recombinación, dos rondas de división nuclear sin síntesis de DNA entre las dos y una segregación cromosómica reduccional. Rem1 es una ciclina que sólo se expresa en meiosis en la levadura de fisión Schizosaccharomyces pombe. Celulas con rem1 deleccionado presentan una tasa de recombinación intragénica disminuida y un retraso en el inicio de meiosis I. Cuando se expresa ectópicamente en células creciendo vegetativamente, Rem1 induce un arresto en G1 seguido de catástrofe mitótica. Este trabajo describe que la expresión de rem1 está regulada a nivel de la trascripción y el procesamiento, codificando para dos proteínas con funciones diferentes dependiendo de la retención intrónica.. Hemos determinado que la regulación del splicing de rem1 no depende de ninguna región transcrita del gen. Además, cuando el promotor se fusiona a otros genes que contienen intrones, las quimeras presentan una regulación específica de meiosis como el rem1 endógeno. Esta regulación depende de dos factores de transcripción de la familia Forkhead, Mei4 y Fkh2. Mientras Mei4 induce la transcripción y el splicing de rem1, Fkh2 es responsable de la retención intrónica del tránscrito durante crecimiento vegetativo y fase S pre-meiótica.Programa de doctorat en BiomedicinaUniversitat Pompeu FabraAyté del Olmo, JoséUniversitat Pompeu Fabra. Departament de Ciències Experimentals i de la Salut2011200920082009info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://www.tdx.cat/TDX-0518109-102004http://hdl.handle.net/10803/7125TDX (Tesis Doctorals en Xarxa)reponame:TDR. Tesis Doctorales en Redinstname:CBUC, CESCAInglésADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.info:eu-repo/semantics/openAccessoai:www.tdx.cat:10803/71252026-06-14T12:46:07Z
score 15,300719

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