Embryo development of prepubertal goat oocytes fertilised by intracytoplasmic sperm injection (ICSI) according to oocyte diameter

The aim of this study was to evaluate embryo development of prepubertal goat oocytes fertilised by ICSI according to their diameter. Three experiments were carried out to achieve this objective. In all experiments, oocytes were matured in TCM199 supplemented with hormones, cysteamine and serum for 2...

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Authors: Jiménez y de Macedo, Ana Raquel, Anguita Bustamante, Begoña, Izquierdo Tugas, Ma. Dolors|||0000-0002-1954-2868, Mogas Amorós, Teresa|||0000-0002-6733-1328, Paramio, María Teresa|||0000-0002-3653-654X
Format: article
Publication Date:2006
Country:España
Institution:Universitat Autònoma de Barcelona
Repository:Dipòsit Digital de Documents de la UAB
Language:English
OAI Identifier:oai:ddd.uab.cat:129362
Online Access:https://ddd.uab.cat/record/129362
https://dx.doi.org/urn:doi:10.1016/j.theriogenology.2006.03.002
Access Level:Open access
Keyword:Cabres
Embriologia veterinària
ICSI
Oocyte diameter
Goat
Prepubertal
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spelling Embryo development of prepubertal goat oocytes fertilised by intracytoplasmic sperm injection (ICSI) according to oocyte diameterJiménez y de Macedo, Ana RaquelAnguita Bustamante, BegoñaIzquierdo Tugas, Ma. Dolors|||0000-0002-1954-2868Mogas Amorós, Teresa|||0000-0002-6733-1328Paramio, María Teresa|||0000-0002-3653-654XCabresEmbriologia veterinàriaICSIOocyte diameterGoatPrepubertalThe aim of this study was to evaluate embryo development of prepubertal goat oocytes fertilised by ICSI according to their diameter. Three experiments were carried out to achieve this objective. In all experiments, oocytes were matured in TCM199 supplemented with hormones, cysteamine and serum for 27 h at 38.58 C. In Experiment 1, we studied the nuclear stage of goat zygotes produced by conventional ICSI and IVF using 20 nM ionomycin plus 10 m M heparin as sperm treatment. A group of Shaminjected oocytes was used as control. Results showed differences in the percentage of 2 PN (zygotes with male and female pronuclei) between ICSI, IVF and Sham (40.9, 26.6 and 3.0%, respectively; P < 0.05). In Experiment 2, we evaluated the embryo development of prepubertal goat oocytes produced by ICSI and IVF after 192 h of culture in SOF medium. The percentage of morulae plus blastocysts obtained was higher in the ICSI than in the IVF group (13.4 and 5.1%, respectively; P < 0.05). In Experiment 3, IVM-oocytes were classified in four groups depending on their diameter (Group A: < 110 m m; Group B: 110-125 mm; Group C: 125-135 mm; Group D: > 135 mm), fertilised by ICSI and cultured for 192 h. Results showed a positive correlation between oocyte diameter and embryo development (morulae + blastocysts: Group A: 0%; Group B: 6.2%; Group C: 46.4% and Group D: 33.3%). In conclusion, sperm treatment with ionomycin plus heparin using the conventional ICSI protocol improved fertilisation rates in comparison to IVF. Oocytes smaller than 125 mm were unable to develop up to blastocyst stage. 22006-01-0120062006-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/129362https://dx.doi.org/urn:doi:10.1016/j.theriogenology.2006.03.002reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1293622026-06-06T12:50:31Z
dc.title.none.fl_str_mv Embryo development of prepubertal goat oocytes fertilised by intracytoplasmic sperm injection (ICSI) according to oocyte diameter
title Embryo development of prepubertal goat oocytes fertilised by intracytoplasmic sperm injection (ICSI) according to oocyte diameter
spellingShingle Embryo development of prepubertal goat oocytes fertilised by intracytoplasmic sperm injection (ICSI) according to oocyte diameter
Jiménez y de Macedo, Ana Raquel
Cabres
Embriologia veterinària
ICSI
Oocyte diameter
Goat
Prepubertal
title_short Embryo development of prepubertal goat oocytes fertilised by intracytoplasmic sperm injection (ICSI) according to oocyte diameter
title_full Embryo development of prepubertal goat oocytes fertilised by intracytoplasmic sperm injection (ICSI) according to oocyte diameter
title_fullStr Embryo development of prepubertal goat oocytes fertilised by intracytoplasmic sperm injection (ICSI) according to oocyte diameter
title_full_unstemmed Embryo development of prepubertal goat oocytes fertilised by intracytoplasmic sperm injection (ICSI) according to oocyte diameter
title_sort Embryo development of prepubertal goat oocytes fertilised by intracytoplasmic sperm injection (ICSI) according to oocyte diameter
dc.creator.none.fl_str_mv Jiménez y de Macedo, Ana Raquel
Anguita Bustamante, Begoña
Izquierdo Tugas, Ma. Dolors|||0000-0002-1954-2868
Mogas Amorós, Teresa|||0000-0002-6733-1328
Paramio, María Teresa|||0000-0002-3653-654X
author Jiménez y de Macedo, Ana Raquel
author_facet Jiménez y de Macedo, Ana Raquel
Anguita Bustamante, Begoña
Izquierdo Tugas, Ma. Dolors|||0000-0002-1954-2868
Mogas Amorós, Teresa|||0000-0002-6733-1328
Paramio, María Teresa|||0000-0002-3653-654X
author_role author
author2 Anguita Bustamante, Begoña
Izquierdo Tugas, Ma. Dolors|||0000-0002-1954-2868
Mogas Amorós, Teresa|||0000-0002-6733-1328
Paramio, María Teresa|||0000-0002-3653-654X
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cabres
Embriologia veterinària
ICSI
Oocyte diameter
Goat
Prepubertal
topic Cabres
Embriologia veterinària
ICSI
Oocyte diameter
Goat
Prepubertal
description The aim of this study was to evaluate embryo development of prepubertal goat oocytes fertilised by ICSI according to their diameter. Three experiments were carried out to achieve this objective. In all experiments, oocytes were matured in TCM199 supplemented with hormones, cysteamine and serum for 27 h at 38.58 C. In Experiment 1, we studied the nuclear stage of goat zygotes produced by conventional ICSI and IVF using 20 nM ionomycin plus 10 m M heparin as sperm treatment. A group of Shaminjected oocytes was used as control. Results showed differences in the percentage of 2 PN (zygotes with male and female pronuclei) between ICSI, IVF and Sham (40.9, 26.6 and 3.0%, respectively; P < 0.05). In Experiment 2, we evaluated the embryo development of prepubertal goat oocytes produced by ICSI and IVF after 192 h of culture in SOF medium. The percentage of morulae plus blastocysts obtained was higher in the ICSI than in the IVF group (13.4 and 5.1%, respectively; P < 0.05). In Experiment 3, IVM-oocytes were classified in four groups depending on their diameter (Group A: < 110 m m; Group B: 110-125 mm; Group C: 125-135 mm; Group D: > 135 mm), fertilised by ICSI and cultured for 192 h. Results showed a positive correlation between oocyte diameter and embryo development (morulae + blastocysts: Group A: 0%; Group B: 6.2%; Group C: 46.4% and Group D: 33.3%). In conclusion, sperm treatment with ionomycin plus heparin using the conventional ICSI protocol improved fertilisation rates in comparison to IVF. Oocytes smaller than 125 mm were unable to develop up to blastocyst stage.
publishDate 2006
dc.date.none.fl_str_mv 2
2006-01-01
2006
2006-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/129362
https://dx.doi.org/urn:doi:10.1016/j.theriogenology.2006.03.002
url https://ddd.uab.cat/record/129362
https://dx.doi.org/urn:doi:10.1016/j.theriogenology.2006.03.002
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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