| Sumario: | [Background] Typical hydrolysis times of saponins generally do not take into consideration the effect of time on the degradation of the target compounds, namely sapogenins. When producing natural extracts, it should be borne in mind that conducting hydrolysis to yield a target compound might also affect the final composition of the extracts in terms of other bioactive compounds. In our study, saponin‐rich extracts from fenugreek, quinoa, lentil, and soybean were produced and their acid hydrolysis to give sapogenin‐rich extracts was conducted over different periods (0–6 h). The disappearance of saponins and appearance of sapogenins was analyzed using high‐performance liquid chromatography–diode array detection–mass spectrometry (HPLC‐DAD‐MS) and gas chromatography–mass spectrometry (GC‐MS), respectively. The impact of hydrolysis on the phytosterols and tocopherol in the extracts was also evaluated.
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