Long noncoding RNAs with enhancer-like function in human cells

While the long noncoding RNAs (ncRNAs) constitute a large portion of the mammalian transcriptome, their biological functions has remained elusive. A few long ncRNAs that have been studied in any detail silence gene expression in processes such as X-inactivation and imprinting. We used a GENCODE anno...

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Detalles Bibliográficos
Autores: Orom, Ulf Andersson, Derrien, Thomas, Beringer, Malte, Gumireddy, Kiranmai, Gardini, Alessandro, Bussotti, Giovanni, 1983-, Lai, Fan, Zytnicki, Matthias, Notredame, Cedric, Huang, Qihong, Guigó Serra, Roderic, Shiekhattar, Ramin
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/25539
Acceso en línea:http://hdl.handle.net/10230/25539
http://dx.doi.org/10.1016/j.cell.2010.09.001
Access Level:acceso abierto
Palabra clave:RNA missatger
Factors de transcripció
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spelling Long noncoding RNAs with enhancer-like function in human cellsOrom, Ulf AnderssonDerrien, ThomasBeringer, MalteGumireddy, KiranmaiGardini, AlessandroBussotti, Giovanni, 1983-Lai, FanZytnicki, MatthiasNotredame, CedricHuang, QihongGuigó Serra, RodericShiekhattar, RaminRNA missatgerFactors de transcripcióWhile the long noncoding RNAs (ncRNAs) constitute a large portion of the mammalian transcriptome, their biological functions has remained elusive. A few long ncRNAs that have been studied in any detail silence gene expression in processes such as X-inactivation and imprinting. We used a GENCODE annotation of the human genome to characterize over a thousand long ncRNAs that are expressed in multiple cell lines. Unexpectedly, we found an enhancer-like function for a set of these long ncRNAs in human cell lines. Depletion of a number of ncRNAs led to decreased expression of their neighboring protein-coding genes, including the master regulator of hematopoiesis, SCL (also called TAL1), Snai1 and Snai2. Using heterologous transcription assays we demonstrated a requirement for the ncRNAs in activation of gene expression. These results reveal an unanticipated role for a class of long ncRNAs in activation of critical regulators of development and differentiation.Elsevier201620162010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/25539http://dx.doi.org/10.1016/j.cell.2010.09.001reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésCell. 2010; 143(1): 46-58© Elsevier. This is the published version of an article http://dx.doi.org/10.1016/j.cell.2010.09.001 that appeared in the journal Cell. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: http://www.elsevier.com/about/open-access/open-access-policies/oa-license-policy/elsevier-user-licenseinfo:eu-repo/semantics/openAccessoai:recercat.cat:10230/255392026-05-29T05:05:01Z
dc.title.none.fl_str_mv Long noncoding RNAs with enhancer-like function in human cells
title Long noncoding RNAs with enhancer-like function in human cells
spellingShingle Long noncoding RNAs with enhancer-like function in human cells
Orom, Ulf Andersson
RNA missatger
Factors de transcripció
title_short Long noncoding RNAs with enhancer-like function in human cells
title_full Long noncoding RNAs with enhancer-like function in human cells
title_fullStr Long noncoding RNAs with enhancer-like function in human cells
title_full_unstemmed Long noncoding RNAs with enhancer-like function in human cells
title_sort Long noncoding RNAs with enhancer-like function in human cells
dc.creator.none.fl_str_mv Orom, Ulf Andersson
Derrien, Thomas
Beringer, Malte
Gumireddy, Kiranmai
Gardini, Alessandro
Bussotti, Giovanni, 1983-
Lai, Fan
Zytnicki, Matthias
Notredame, Cedric
Huang, Qihong
Guigó Serra, Roderic
Shiekhattar, Ramin
author Orom, Ulf Andersson
author_facet Orom, Ulf Andersson
Derrien, Thomas
Beringer, Malte
Gumireddy, Kiranmai
Gardini, Alessandro
Bussotti, Giovanni, 1983-
Lai, Fan
Zytnicki, Matthias
Notredame, Cedric
Huang, Qihong
Guigó Serra, Roderic
Shiekhattar, Ramin
author_role author
author2 Derrien, Thomas
Beringer, Malte
Gumireddy, Kiranmai
Gardini, Alessandro
Bussotti, Giovanni, 1983-
Lai, Fan
Zytnicki, Matthias
Notredame, Cedric
Huang, Qihong
Guigó Serra, Roderic
Shiekhattar, Ramin
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv RNA missatger
Factors de transcripció
topic RNA missatger
Factors de transcripció
description While the long noncoding RNAs (ncRNAs) constitute a large portion of the mammalian transcriptome, their biological functions has remained elusive. A few long ncRNAs that have been studied in any detail silence gene expression in processes such as X-inactivation and imprinting. We used a GENCODE annotation of the human genome to characterize over a thousand long ncRNAs that are expressed in multiple cell lines. Unexpectedly, we found an enhancer-like function for a set of these long ncRNAs in human cell lines. Depletion of a number of ncRNAs led to decreased expression of their neighboring protein-coding genes, including the master regulator of hematopoiesis, SCL (also called TAL1), Snai1 and Snai2. Using heterologous transcription assays we demonstrated a requirement for the ncRNAs in activation of gene expression. These results reveal an unanticipated role for a class of long ncRNAs in activation of critical regulators of development and differentiation.
publishDate 2010
dc.date.none.fl_str_mv 2010
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/25539
http://dx.doi.org/10.1016/j.cell.2010.09.001
url http://hdl.handle.net/10230/25539
http://dx.doi.org/10.1016/j.cell.2010.09.001
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Cell. 2010; 143(1): 46-58
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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