Effect of Crocetin on Basal Lipolysis in 3T3-L1 Adipocytes

Crocetin (CCT) is a natural saffron-derived apocarotenoid that possesses healthy properties such as anti-adipogenic, anti-inflammatory, and antioxidant activities. Lipolysis is enhanced in obesity and correlates with a pro-inflammatory, pro-oxidant state. In this context, we aimed to investigate whe...

Descripción completa

Detalles Bibliográficos
Autores: Cimas Felipe, Francisco José, Cruz Morcillo, Miguel Ángel de la, Cifuentes Verdú, María Carmen, Moratalla López, Natalia, Alonso Díaz-Marta, Gonzalo Luis, Nava Hernández, Eduardo, Llorens Folgado, Silvia
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad de Castilla-La Mancha
Repositorio:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/32006
Acceso en línea:https://doi.org/10.3390/antiox12061254
https://hdl.handle.net/10578/32006
Access Level:acceso abierto
Palabra clave:crocetin
lipolysis
ATGL
HSL
perilipin-1
catalase
superoxide dismutase
adiponectin
resistin
NOS2
adipocytes
3T3-L1
id ES_8f8a7220f3900237f2bf53119d9bd4e4
oai_identifier_str oai:ruidera.uclm.es:10578/32006
network_acronym_str ES
network_name_str España
repository_id_str
spelling Effect of Crocetin on Basal Lipolysis in 3T3-L1 AdipocytesCimas Felipe, Francisco JoséCruz Morcillo, Miguel Ángel de laCifuentes Verdú, María CarmenMoratalla López, NataliaAlonso Díaz-Marta, Gonzalo LuisNava Hernández, EduardoLlorens Folgado, SilviacrocetinlipolysisATGLHSLperilipin-1catalasesuperoxide dismutaseadiponectinresistinNOS2adipocytes3T3-L1Crocetin (CCT) is a natural saffron-derived apocarotenoid that possesses healthy properties such as anti-adipogenic, anti-inflammatory, and antioxidant activities. Lipolysis is enhanced in obesity and correlates with a pro-inflammatory, pro-oxidant state. In this context, we aimed to investigate whether CCT affects lipolysis. To evaluate CCT’s possible lipolytic effect, 3T3-L1 adipocytes were treated with CCT10microM at day 5 post-differentiation. Glycerol content and antioxidant activity were assessed using colorimetric assays. Gene expression was measured using qRT-PCR to evaluate the effect of CCT on key lipolytic enzymes and on nitric oxide synthase (NOS) expression. Total lipid accumulation was assessed using Oil Red O staining. CCT10microM decreased glycerol release from 3T3-L1 adipocytes and downregulated adipose tissue triglyceride lipase (ATGL) and perilipin-1, but not hormone-sensitive lipase (HSL), suggesting an anti-lipolytic effect. CCT increased catalase (CAT) and superoxide dismutase (SOD) activity, thus showing an antioxidant effect. In addition, CCT exhibited an anti-inflammatory profile, i.e., diminished inducible NOS (NOS2) and resistin expression, while enhancing the expression of adiponectin. CCT10microM also decreased intracellular fat and C/EBPalpha expression (a transcription factor involved in adipogenesis), thus revealing an anti-adipogenic effect. These findings point to CCT as a promising biocompound for improving lipid mobilisation in obesity.MDPI202320232023info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://doi.org/10.3390/antiox12061254https://hdl.handle.net/10578/32006reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglés2019-GRIN-27019 co-funded by EU FEDER-ERDF2020-GRIN-29186 co-funded by EU FEDER-ERDF2023-GRIN-34301 co-funded by EU FEDER-ERDFinfo:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/320062026-05-27T07:36:41Z
dc.title.none.fl_str_mv Effect of Crocetin on Basal Lipolysis in 3T3-L1 Adipocytes
title Effect of Crocetin on Basal Lipolysis in 3T3-L1 Adipocytes
spellingShingle Effect of Crocetin on Basal Lipolysis in 3T3-L1 Adipocytes
Cimas Felipe, Francisco José
crocetin
lipolysis
ATGL
HSL
perilipin-1
catalase
superoxide dismutase
adiponectin
resistin
NOS2
adipocytes
3T3-L1
title_short Effect of Crocetin on Basal Lipolysis in 3T3-L1 Adipocytes
title_full Effect of Crocetin on Basal Lipolysis in 3T3-L1 Adipocytes
title_fullStr Effect of Crocetin on Basal Lipolysis in 3T3-L1 Adipocytes
title_full_unstemmed Effect of Crocetin on Basal Lipolysis in 3T3-L1 Adipocytes
title_sort Effect of Crocetin on Basal Lipolysis in 3T3-L1 Adipocytes
dc.creator.none.fl_str_mv Cimas Felipe, Francisco José
Cruz Morcillo, Miguel Ángel de la
Cifuentes Verdú, María Carmen
Moratalla López, Natalia
Alonso Díaz-Marta, Gonzalo Luis
Nava Hernández, Eduardo
Llorens Folgado, Silvia
author Cimas Felipe, Francisco José
author_facet Cimas Felipe, Francisco José
Cruz Morcillo, Miguel Ángel de la
Cifuentes Verdú, María Carmen
Moratalla López, Natalia
Alonso Díaz-Marta, Gonzalo Luis
Nava Hernández, Eduardo
Llorens Folgado, Silvia
author_role author
author2 Cruz Morcillo, Miguel Ángel de la
Cifuentes Verdú, María Carmen
Moratalla López, Natalia
Alonso Díaz-Marta, Gonzalo Luis
Nava Hernández, Eduardo
Llorens Folgado, Silvia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv crocetin
lipolysis
ATGL
HSL
perilipin-1
catalase
superoxide dismutase
adiponectin
resistin
NOS2
adipocytes
3T3-L1
topic crocetin
lipolysis
ATGL
HSL
perilipin-1
catalase
superoxide dismutase
adiponectin
resistin
NOS2
adipocytes
3T3-L1
description Crocetin (CCT) is a natural saffron-derived apocarotenoid that possesses healthy properties such as anti-adipogenic, anti-inflammatory, and antioxidant activities. Lipolysis is enhanced in obesity and correlates with a pro-inflammatory, pro-oxidant state. In this context, we aimed to investigate whether CCT affects lipolysis. To evaluate CCT’s possible lipolytic effect, 3T3-L1 adipocytes were treated with CCT10microM at day 5 post-differentiation. Glycerol content and antioxidant activity were assessed using colorimetric assays. Gene expression was measured using qRT-PCR to evaluate the effect of CCT on key lipolytic enzymes and on nitric oxide synthase (NOS) expression. Total lipid accumulation was assessed using Oil Red O staining. CCT10microM decreased glycerol release from 3T3-L1 adipocytes and downregulated adipose tissue triglyceride lipase (ATGL) and perilipin-1, but not hormone-sensitive lipase (HSL), suggesting an anti-lipolytic effect. CCT increased catalase (CAT) and superoxide dismutase (SOD) activity, thus showing an antioxidant effect. In addition, CCT exhibited an anti-inflammatory profile, i.e., diminished inducible NOS (NOS2) and resistin expression, while enhancing the expression of adiponectin. CCT10microM also decreased intracellular fat and C/EBPalpha expression (a transcription factor involved in adipogenesis), thus revealing an anti-adipogenic effect. These findings point to CCT as a promising biocompound for improving lipid mobilisation in obesity.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://doi.org/10.3390/antiox12061254
https://hdl.handle.net/10578/32006
url https://doi.org/10.3390/antiox12061254
https://hdl.handle.net/10578/32006
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv 2019-GRIN-27019 co-funded by EU FEDER-ERDF
2020-GRIN-29186 co-funded by EU FEDER-ERDF
2023-GRIN-34301 co-funded by EU FEDER-ERDF
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:RUIdeRA. Repositorio Institucional de la UCLM
instname:Universidad de Castilla-La Mancha
instname_str Universidad de Castilla-La Mancha
reponame_str RUIdeRA. Repositorio Institucional de la UCLM
collection RUIdeRA. Repositorio Institucional de la UCLM
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869413220837490688
score 15.301603

Similares en LA Referencia