Biodegradable particles for protein delivery: Estimation of the release kinetics inside cells

A methodology to quantify the efficiency of the protein loading and in-vitro delivery for biodegradable capsules with different architectures based on polyelectrolytes (dextran sulfate, poly-L-arginine and polyethylenimine) and SiO was developed. The capsules were loaded with model proteins such as...

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Detalhes bibliográficos
Autores: Zyuzin, M.V., Hartmann, R, Timin, A.S, Carregal-Romero, S., Parak, W.J., Escudero, A.
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/364393
Acesso em linha:http://hdl.handle.net/10261/364393
Access Level:Acceso aberto
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spelling Biodegradable particles for protein delivery: Estimation of the release kinetics inside cellsZyuzin, M.V.Hartmann, RTimin, A.SCarregal-Romero, S.Parak, W.J.Escudero, A.A methodology to quantify the efficiency of the protein loading and in-vitro delivery for biodegradable capsules with different architectures based on polyelectrolytes (dextran sulfate, poly-L-arginine and polyethylenimine) and SiO was developed. The capsules were loaded with model proteins such as ovalbumin and green fluorescent protein (GFP), and the protein release profile inside cells (either macrophages or HeLa cells) after endocytosis was analysed. Both, protein loading and release kinetics were evaluated by analysing confocal laser scanning microscopy images using MatLab and CellProfiler software. Our results indicate that silica capsules showed the most efficient release of proteins as cargo molecules within 48 h, as compared to their polymeric counterparts. This developed method for the analysis of the intracellular cargo release kinetics from carrier structures could be used in the future for a better control of drug release profiles.ElsevierMinisterio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420222024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/364393reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.bioadv.2022.212966Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3643932026-05-22T06:33:51Z
dc.title.none.fl_str_mv Biodegradable particles for protein delivery: Estimation of the release kinetics inside cells
title Biodegradable particles for protein delivery: Estimation of the release kinetics inside cells
spellingShingle Biodegradable particles for protein delivery: Estimation of the release kinetics inside cells
Zyuzin, M.V.
title_short Biodegradable particles for protein delivery: Estimation of the release kinetics inside cells
title_full Biodegradable particles for protein delivery: Estimation of the release kinetics inside cells
title_fullStr Biodegradable particles for protein delivery: Estimation of the release kinetics inside cells
title_full_unstemmed Biodegradable particles for protein delivery: Estimation of the release kinetics inside cells
title_sort Biodegradable particles for protein delivery: Estimation of the release kinetics inside cells
dc.creator.none.fl_str_mv Zyuzin, M.V.
Hartmann, R
Timin, A.S
Carregal-Romero, S.
Parak, W.J.
Escudero, A.
author Zyuzin, M.V.
author_facet Zyuzin, M.V.
Hartmann, R
Timin, A.S
Carregal-Romero, S.
Parak, W.J.
Escudero, A.
author_role author
author2 Hartmann, R
Timin, A.S
Carregal-Romero, S.
Parak, W.J.
Escudero, A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description A methodology to quantify the efficiency of the protein loading and in-vitro delivery for biodegradable capsules with different architectures based on polyelectrolytes (dextran sulfate, poly-L-arginine and polyethylenimine) and SiO was developed. The capsules were loaded with model proteins such as ovalbumin and green fluorescent protein (GFP), and the protein release profile inside cells (either macrophages or HeLa cells) after endocytosis was analysed. Both, protein loading and release kinetics were evaluated by analysing confocal laser scanning microscopy images using MatLab and CellProfiler software. Our results indicate that silica capsules showed the most efficient release of proteins as cargo molecules within 48 h, as compared to their polymeric counterparts. This developed method for the analysis of the intracellular cargo release kinetics from carrier structures could be used in the future for a better control of drug release profiles.
publishDate 2022
dc.date.none.fl_str_mv 2022
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/364393
url http://hdl.handle.net/10261/364393
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.bioadv.2022.212966
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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